Effects of Garlic Oil on the Migration of Neutrophil-Like Cell Studied by Using a Chemotactic Gradient Labchip
We have designed and fabricated a novel chemotactic gradient Labchip for studying cell migration quantitatively. Owing to the great potential of garlic and its preparations in developing antiinflammatory drugs, the aim of the present study is to investigate the effect of garlic oil on the locomotion...
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Online Access: | http://dx.doi.org/10.1155/2010/319059 |
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doaj-0ae1840ad2e44127a347825ea2f9c6e72020-11-25T00:53:46ZengHindawi LimitedJournal of Biomedicine and Biotechnology1110-72431110-72512010-01-01201010.1155/2010/319059319059Effects of Garlic Oil on the Migration of Neutrophil-Like Cell Studied by Using a Chemotactic Gradient LabchipPo-Chen Shih0Chia-Hao Kuo1Jenh-Yih Juang2Cheng-Hsien Liu3Long Hsu4Cheng-Tzu Liu5Department of Electrophysics, National Chiao Tung University, Hsinchu 300, TaiwanSchool of Nutrition, Chung Shan Medical University, Taichung 402, TaiwanDepartment of Electrophysics, National Chiao Tung University, Hsinchu 300, TaiwanDepartment of Power Mechanical Engineering, National Tsing Hua University, Hsinchu 300, TaiwanDepartment of Electrophysics, National Chiao Tung University, Hsinchu 300, TaiwanSchool of Nutrition, Chung Shan Medical University, Taichung 402, TaiwanWe have designed and fabricated a novel chemotactic gradient Labchip for studying cell migration quantitatively. Owing to the great potential of garlic and its preparations in developing antiinflammatory drugs, the aim of the present study is to investigate the effect of garlic oil on the locomotion of a neutrophil-like cell by measuring the dynamic features of cell migration including migration direction, average migration speed, chemotactic index (CI), and motility index (MI) with the newly designed Labchip. We found that garlic oil treatment lowered the values of CI and MI and reduced the average speed of cell migration from 13 to 8 μm/min. The results indicate that garlic oil is a potential inhibitor for neutrophil-like cell migration and chemotactic responsiveness. By comparing with the effects of nocodazole and cytochalasin B, we also suggest that the antiinflammatory activity exhibited by garlic oil was mainly through inhibiting the assembly-disassembly processes of the cytoskeleton.http://dx.doi.org/10.1155/2010/319059 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Po-Chen Shih Chia-Hao Kuo Jenh-Yih Juang Cheng-Hsien Liu Long Hsu Cheng-Tzu Liu |
spellingShingle |
Po-Chen Shih Chia-Hao Kuo Jenh-Yih Juang Cheng-Hsien Liu Long Hsu Cheng-Tzu Liu Effects of Garlic Oil on the Migration of Neutrophil-Like Cell Studied by Using a Chemotactic Gradient Labchip Journal of Biomedicine and Biotechnology |
author_facet |
Po-Chen Shih Chia-Hao Kuo Jenh-Yih Juang Cheng-Hsien Liu Long Hsu Cheng-Tzu Liu |
author_sort |
Po-Chen Shih |
title |
Effects of Garlic Oil on the Migration of Neutrophil-Like Cell Studied by Using a Chemotactic Gradient Labchip |
title_short |
Effects of Garlic Oil on the Migration of Neutrophil-Like Cell Studied by Using a Chemotactic Gradient Labchip |
title_full |
Effects of Garlic Oil on the Migration of Neutrophil-Like Cell Studied by Using a Chemotactic Gradient Labchip |
title_fullStr |
Effects of Garlic Oil on the Migration of Neutrophil-Like Cell Studied by Using a Chemotactic Gradient Labchip |
title_full_unstemmed |
Effects of Garlic Oil on the Migration of Neutrophil-Like Cell Studied by Using a Chemotactic Gradient Labchip |
title_sort |
effects of garlic oil on the migration of neutrophil-like cell studied by using a chemotactic gradient labchip |
publisher |
Hindawi Limited |
series |
Journal of Biomedicine and Biotechnology |
issn |
1110-7243 1110-7251 |
publishDate |
2010-01-01 |
description |
We have designed and fabricated a novel chemotactic gradient Labchip for studying cell migration quantitatively. Owing to the great potential of garlic and its preparations in developing antiinflammatory drugs, the aim of the present study is to investigate the effect of garlic oil on the locomotion of a neutrophil-like cell by measuring the dynamic features of cell migration including migration direction, average migration speed, chemotactic index (CI), and motility index (MI) with the newly designed Labchip. We found that garlic oil treatment lowered the values of CI and MI and reduced the average speed of cell migration from 13 to 8 μm/min. The results indicate that garlic oil is a potential inhibitor for neutrophil-like cell migration and chemotactic responsiveness. By comparing with the effects of nocodazole and cytochalasin B, we also suggest that the antiinflammatory activity exhibited by garlic oil was mainly through inhibiting the assembly-disassembly processes of the cytoskeleton. |
url |
http://dx.doi.org/10.1155/2010/319059 |
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