Using next generation sequencing of alpine plants to improve fecal metabarcoding diet analysis for Dall’s sheep
Abstract Objectives Dall’s sheep (Ovis dalli dalli) are important herbivores in the mountainous ecosystems of northwestern North America, and recent declines in some populations have sparked concern. Our aim was to improve capabilities for fecal metabarcoding diet analysis of Dall’s sheep and other...
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2021-05-01
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| Series: | BMC Research Notes |
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| Online Access: | https://doi.org/10.1186/s13104-021-05590-z |
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doaj-0ae5d92ea79845be8b26b2a862c4aaba2021-05-09T11:39:40ZengBMCBMC Research Notes1756-05002021-05-011411410.1186/s13104-021-05590-zUsing next generation sequencing of alpine plants to improve fecal metabarcoding diet analysis for Dall’s sheepKelly E. Williams0Damian M. Menning1Eric J. Wald2Sandra L. Talbot3Kumi L. Rattenbury4Laura R. Prugh5School of Environmental and Forest Sciences, University of WashingtonU.S. Geological Survey, Alaska Science CenterArctic Network Inventory & Monitoring Division, National Park ServiceU.S. Geological Survey, Alaska Science CenterArctic Network Inventory & Monitoring Division, National Park ServiceSchool of Environmental and Forest Sciences, University of WashingtonAbstract Objectives Dall’s sheep (Ovis dalli dalli) are important herbivores in the mountainous ecosystems of northwestern North America, and recent declines in some populations have sparked concern. Our aim was to improve capabilities for fecal metabarcoding diet analysis of Dall’s sheep and other herbivores by contributing new sequence data for arctic and alpine plants. This expanded reference library will provide critical reference sequence data that will facilitate metabarcoding diet analysis of Dall’s sheep and thus improve understanding of plant-animal interactions in a region undergoing rapid climate change. Data description We provide sequences for the chloroplast rbcL gene of 16 arctic-alpine vascular plant species that are known to comprise the diet of Dall’s sheep. These sequences contribute to a growing reference library that can be used in diet studies of arctic herbivores.https://doi.org/10.1186/s13104-021-05590-zAlpineArcticBorealDall’s sheepDietFecal |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Kelly E. Williams Damian M. Menning Eric J. Wald Sandra L. Talbot Kumi L. Rattenbury Laura R. Prugh |
| spellingShingle |
Kelly E. Williams Damian M. Menning Eric J. Wald Sandra L. Talbot Kumi L. Rattenbury Laura R. Prugh Using next generation sequencing of alpine plants to improve fecal metabarcoding diet analysis for Dall’s sheep BMC Research Notes Alpine Arctic Boreal Dall’s sheep Diet Fecal |
| author_facet |
Kelly E. Williams Damian M. Menning Eric J. Wald Sandra L. Talbot Kumi L. Rattenbury Laura R. Prugh |
| author_sort |
Kelly E. Williams |
| title |
Using next generation sequencing of alpine plants to improve fecal metabarcoding diet analysis for Dall’s sheep |
| title_short |
Using next generation sequencing of alpine plants to improve fecal metabarcoding diet analysis for Dall’s sheep |
| title_full |
Using next generation sequencing of alpine plants to improve fecal metabarcoding diet analysis for Dall’s sheep |
| title_fullStr |
Using next generation sequencing of alpine plants to improve fecal metabarcoding diet analysis for Dall’s sheep |
| title_full_unstemmed |
Using next generation sequencing of alpine plants to improve fecal metabarcoding diet analysis for Dall’s sheep |
| title_sort |
using next generation sequencing of alpine plants to improve fecal metabarcoding diet analysis for dall’s sheep |
| publisher |
BMC |
| series |
BMC Research Notes |
| issn |
1756-0500 |
| publishDate |
2021-05-01 |
| description |
Abstract Objectives Dall’s sheep (Ovis dalli dalli) are important herbivores in the mountainous ecosystems of northwestern North America, and recent declines in some populations have sparked concern. Our aim was to improve capabilities for fecal metabarcoding diet analysis of Dall’s sheep and other herbivores by contributing new sequence data for arctic and alpine plants. This expanded reference library will provide critical reference sequence data that will facilitate metabarcoding diet analysis of Dall’s sheep and thus improve understanding of plant-animal interactions in a region undergoing rapid climate change. Data description We provide sequences for the chloroplast rbcL gene of 16 arctic-alpine vascular plant species that are known to comprise the diet of Dall’s sheep. These sequences contribute to a growing reference library that can be used in diet studies of arctic herbivores. |
| topic |
Alpine Arctic Boreal Dall’s sheep Diet Fecal |
| url |
https://doi.org/10.1186/s13104-021-05590-z |
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