Evaluation of performance of two SARS-CoV-2 Rapid IgM-IgG combined antibody tests on capillary whole blood samples from the fingertip.
<h4>Background</h4>The SARS-CoV-2 (Severe Acute Respiratory Syndrome CoronaVirus 2) is responsible for the infectious respiratory disease called COVID-19 (COronaVIrus Disease 2019). In response to the growing COVID-19 pandemic, point-of-care (POC) tests have been developed to detect spec...
Main Authors: | , , , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2020-01-01
|
Series: | PLoS ONE |
Online Access: | https://doi.org/10.1371/journal.pone.0237694 |
id |
doaj-0b02c4f3e8d04a008a5fb176b6935727 |
---|---|
record_format |
Article |
spelling |
doaj-0b02c4f3e8d04a008a5fb176b69357272021-03-04T11:53:50ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-01159e023769410.1371/journal.pone.0237694Evaluation of performance of two SARS-CoV-2 Rapid IgM-IgG combined antibody tests on capillary whole blood samples from the fingertip.Thierry PrazuckMathilda ColinSusanna GiachèCamélia GubavuAymeric SeveVincent RzepeckiMarie Chevereau-ChoquetCatherine KianiVictor RodotElsa LionnetLaura CourtellemontJérôme GuinardGilles PialouxLaurent Hocqueloux<h4>Background</h4>The SARS-CoV-2 (Severe Acute Respiratory Syndrome CoronaVirus 2) is responsible for the infectious respiratory disease called COVID-19 (COronaVIrus Disease 2019). In response to the growing COVID-19 pandemic, point-of-care (POC) tests have been developed to detect specific antibodies, IgG and IgM, to SARS-CoV-2 virus in human whole blood. We conducted a prospective observational study to evaluate the performance of two POC tests, COVID-PRESTO® and COVID-DUO®, compared to the gold standard, RT-PCR (real-time reverse transcriptase polymerase chain reaction).<h4>Methods</h4>RT-PCR testing of SARS-Cov-2 was performed from nasopharyngeal swab specimens collected in adult patients visiting the infectious disease department at the hospital (Orléans, France). Capillary whole blood (CWB) samples from the fingertip taken at different time points after onset of the disease were tested with POC tests. The specificity and sensitivity of the rapid test kits compared to test of reference (RT-PCR) were calculated.<h4>Results</h4>Among 381 patients with symptoms of COVID-19 who went to the hospital for a diagnostic, 143 patients were RT-PCR negative. Results of test with POC tests were all negative for these patients, indicating a specificity of 100% for both POC tests. In the RT-PCR positive subgroup (n = 238), 133 patients were tested with COVID-PRESTO® and 129 patients were tested with COVID-DUO® (24 patients tested with both). The further the onset of symptoms was from the date of collection, the greater the sensitivity. The sensitivity of COVID-PRESTO® test ranged from 10.00% for patients having experienced their 1st symptoms from 0 to 5 days ago to 100% in patients where symptoms had occurred more than 15 days before the date of tests. For COVID-DUO® test, the sensitivity ranged from 35.71% [0-5 days] to 100% (> 15 days).<h4>Conclusion</h4>COVID-PRESTO® and DUO® POC tests turned out to be very specific (none false positive) and to be sensitive enough after 15 days from onset of symptom. These easy to use IgG/IgM combined test kits are the first ones allowing a screening with CWB sample, by typing from a finger prick. These rapid tests are particularly interesting for screening in low resource settings.https://doi.org/10.1371/journal.pone.0237694 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Thierry Prazuck Mathilda Colin Susanna Giachè Camélia Gubavu Aymeric Seve Vincent Rzepecki Marie Chevereau-Choquet Catherine Kiani Victor Rodot Elsa Lionnet Laura Courtellemont Jérôme Guinard Gilles Pialoux Laurent Hocqueloux |
spellingShingle |
Thierry Prazuck Mathilda Colin Susanna Giachè Camélia Gubavu Aymeric Seve Vincent Rzepecki Marie Chevereau-Choquet Catherine Kiani Victor Rodot Elsa Lionnet Laura Courtellemont Jérôme Guinard Gilles Pialoux Laurent Hocqueloux Evaluation of performance of two SARS-CoV-2 Rapid IgM-IgG combined antibody tests on capillary whole blood samples from the fingertip. PLoS ONE |
author_facet |
Thierry Prazuck Mathilda Colin Susanna Giachè Camélia Gubavu Aymeric Seve Vincent Rzepecki Marie Chevereau-Choquet Catherine Kiani Victor Rodot Elsa Lionnet Laura Courtellemont Jérôme Guinard Gilles Pialoux Laurent Hocqueloux |
author_sort |
Thierry Prazuck |
title |
Evaluation of performance of two SARS-CoV-2 Rapid IgM-IgG combined antibody tests on capillary whole blood samples from the fingertip. |
title_short |
Evaluation of performance of two SARS-CoV-2 Rapid IgM-IgG combined antibody tests on capillary whole blood samples from the fingertip. |
title_full |
Evaluation of performance of two SARS-CoV-2 Rapid IgM-IgG combined antibody tests on capillary whole blood samples from the fingertip. |
title_fullStr |
Evaluation of performance of two SARS-CoV-2 Rapid IgM-IgG combined antibody tests on capillary whole blood samples from the fingertip. |
title_full_unstemmed |
Evaluation of performance of two SARS-CoV-2 Rapid IgM-IgG combined antibody tests on capillary whole blood samples from the fingertip. |
title_sort |
evaluation of performance of two sars-cov-2 rapid igm-igg combined antibody tests on capillary whole blood samples from the fingertip. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2020-01-01 |
description |
<h4>Background</h4>The SARS-CoV-2 (Severe Acute Respiratory Syndrome CoronaVirus 2) is responsible for the infectious respiratory disease called COVID-19 (COronaVIrus Disease 2019). In response to the growing COVID-19 pandemic, point-of-care (POC) tests have been developed to detect specific antibodies, IgG and IgM, to SARS-CoV-2 virus in human whole blood. We conducted a prospective observational study to evaluate the performance of two POC tests, COVID-PRESTO® and COVID-DUO®, compared to the gold standard, RT-PCR (real-time reverse transcriptase polymerase chain reaction).<h4>Methods</h4>RT-PCR testing of SARS-Cov-2 was performed from nasopharyngeal swab specimens collected in adult patients visiting the infectious disease department at the hospital (Orléans, France). Capillary whole blood (CWB) samples from the fingertip taken at different time points after onset of the disease were tested with POC tests. The specificity and sensitivity of the rapid test kits compared to test of reference (RT-PCR) were calculated.<h4>Results</h4>Among 381 patients with symptoms of COVID-19 who went to the hospital for a diagnostic, 143 patients were RT-PCR negative. Results of test with POC tests were all negative for these patients, indicating a specificity of 100% for both POC tests. In the RT-PCR positive subgroup (n = 238), 133 patients were tested with COVID-PRESTO® and 129 patients were tested with COVID-DUO® (24 patients tested with both). The further the onset of symptoms was from the date of collection, the greater the sensitivity. The sensitivity of COVID-PRESTO® test ranged from 10.00% for patients having experienced their 1st symptoms from 0 to 5 days ago to 100% in patients where symptoms had occurred more than 15 days before the date of tests. For COVID-DUO® test, the sensitivity ranged from 35.71% [0-5 days] to 100% (> 15 days).<h4>Conclusion</h4>COVID-PRESTO® and DUO® POC tests turned out to be very specific (none false positive) and to be sensitive enough after 15 days from onset of symptom. These easy to use IgG/IgM combined test kits are the first ones allowing a screening with CWB sample, by typing from a finger prick. These rapid tests are particularly interesting for screening in low resource settings. |
url |
https://doi.org/10.1371/journal.pone.0237694 |
work_keys_str_mv |
AT thierryprazuck evaluationofperformanceoftwosarscov2rapidigmiggcombinedantibodytestsoncapillarywholebloodsamplesfromthefingertip AT mathildacolin evaluationofperformanceoftwosarscov2rapidigmiggcombinedantibodytestsoncapillarywholebloodsamplesfromthefingertip AT susannagiache evaluationofperformanceoftwosarscov2rapidigmiggcombinedantibodytestsoncapillarywholebloodsamplesfromthefingertip AT cameliagubavu evaluationofperformanceoftwosarscov2rapidigmiggcombinedantibodytestsoncapillarywholebloodsamplesfromthefingertip AT aymericseve evaluationofperformanceoftwosarscov2rapidigmiggcombinedantibodytestsoncapillarywholebloodsamplesfromthefingertip AT vincentrzepecki evaluationofperformanceoftwosarscov2rapidigmiggcombinedantibodytestsoncapillarywholebloodsamplesfromthefingertip AT mariechevereauchoquet evaluationofperformanceoftwosarscov2rapidigmiggcombinedantibodytestsoncapillarywholebloodsamplesfromthefingertip AT catherinekiani evaluationofperformanceoftwosarscov2rapidigmiggcombinedantibodytestsoncapillarywholebloodsamplesfromthefingertip AT victorrodot evaluationofperformanceoftwosarscov2rapidigmiggcombinedantibodytestsoncapillarywholebloodsamplesfromthefingertip AT elsalionnet evaluationofperformanceoftwosarscov2rapidigmiggcombinedantibodytestsoncapillarywholebloodsamplesfromthefingertip AT lauracourtellemont evaluationofperformanceoftwosarscov2rapidigmiggcombinedantibodytestsoncapillarywholebloodsamplesfromthefingertip AT jeromeguinard evaluationofperformanceoftwosarscov2rapidigmiggcombinedantibodytestsoncapillarywholebloodsamplesfromthefingertip AT gillespialoux evaluationofperformanceoftwosarscov2rapidigmiggcombinedantibodytestsoncapillarywholebloodsamplesfromthefingertip AT laurenthocqueloux evaluationofperformanceoftwosarscov2rapidigmiggcombinedantibodytestsoncapillarywholebloodsamplesfromthefingertip |
_version_ |
1714803168422920192 |