Stimulation of Intestinal Cl- Secretion Through CFTR by Caffeine Intake in Salt-Sensitive Hypertensive Rats

Background/Aims: High salt consumption is a major risk factor for hypertension, and sodium homeostasis is regulated by both intestinal sodium absorption and urinary sodium excretion. Chronic caffeine intake has been reported to attenuate salt-sensitive hypertension by promoting urinary sodium excret...

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Main Authors: Xiao Wei, Zongshi Lu, Tao Yang, Peng Gao, Sijiao Chen, Daoyan Liu, Zhiming Zhu
Format: Article
Language:English
Published: Karger Publishers 2018-03-01
Series:Kidney & Blood Pressure Research
Subjects:
Online Access:https://www.karger.com/Article/FullText/488256
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spelling doaj-0b05fb294ab243bfa8ae2c7f8b753d332020-11-25T03:45:06ZengKarger PublishersKidney & Blood Pressure Research1420-40961423-01432018-03-0143243944810.1159/000488256488256Stimulation of Intestinal Cl- Secretion Through CFTR by Caffeine Intake in Salt-Sensitive Hypertensive RatsXiao WeiZongshi LuTao YangPeng GaoSijiao ChenDaoyan LiuZhiming ZhuBackground/Aims: High salt consumption is a major risk factor for hypertension, and sodium homeostasis is regulated by both intestinal sodium absorption and urinary sodium excretion. Chronic caffeine intake has been reported to attenuate salt-sensitive hypertension by promoting urinary sodium excretion; however, its exact role in intestinal sodium absorption remains unknown. Here, we investigated whether and how chronic caffeine consumption antagonizes salt-sensitive hypertension by inhibiting intestinal sodium absorption. Methods: Dahl salt-sensitive rats were fed 8% NaCl chow and 0.1% caffeine in their drinking water for 15 days. The blood pressure and fecal sodium content were measured. The effect of caffeine on the movement of Cl- in enterocyte cells was determined with the Ussing chamber assay. Results: Rats that were treated with caffeine displayed significantly lower mean blood pressure and higher fecal sodium content than the controls. Consistent with these findings, caffeine intake decreased fluid absorption by the intestine in the fluid perfusion experiment. Further, the results from the Ussing chamber assay indicated that caffeine promoted Cl- secretion through enterocyte apical cystic fibrosis transmembrane conductance regulator (CFTR), and thus inhibited sodium absorption. Moreover, depletion of cAMP or inhibition of CFTR completely abolished the effect of caffeine on Cl- secretion. Conclusion: The results indicate that chronic caffeine consumption reduces sodium absorption by promoting CFTR-mediated Cl- secretion in the intestine, which contributes to the anti-hypertensive effect of caffeine in salt-sensitive rats.https://www.karger.com/Article/FullText/488256CaffeineSalt-sensitive hypertensionIntestinal sodium absorptionCFTRCAMP
collection DOAJ
language English
format Article
sources DOAJ
author Xiao Wei
Zongshi Lu
Tao Yang
Peng Gao
Sijiao Chen
Daoyan Liu
Zhiming Zhu
spellingShingle Xiao Wei
Zongshi Lu
Tao Yang
Peng Gao
Sijiao Chen
Daoyan Liu
Zhiming Zhu
Stimulation of Intestinal Cl- Secretion Through CFTR by Caffeine Intake in Salt-Sensitive Hypertensive Rats
Kidney & Blood Pressure Research
Caffeine
Salt-sensitive hypertension
Intestinal sodium absorption
CFTR
CAMP
author_facet Xiao Wei
Zongshi Lu
Tao Yang
Peng Gao
Sijiao Chen
Daoyan Liu
Zhiming Zhu
author_sort Xiao Wei
title Stimulation of Intestinal Cl- Secretion Through CFTR by Caffeine Intake in Salt-Sensitive Hypertensive Rats
title_short Stimulation of Intestinal Cl- Secretion Through CFTR by Caffeine Intake in Salt-Sensitive Hypertensive Rats
title_full Stimulation of Intestinal Cl- Secretion Through CFTR by Caffeine Intake in Salt-Sensitive Hypertensive Rats
title_fullStr Stimulation of Intestinal Cl- Secretion Through CFTR by Caffeine Intake in Salt-Sensitive Hypertensive Rats
title_full_unstemmed Stimulation of Intestinal Cl- Secretion Through CFTR by Caffeine Intake in Salt-Sensitive Hypertensive Rats
title_sort stimulation of intestinal cl- secretion through cftr by caffeine intake in salt-sensitive hypertensive rats
publisher Karger Publishers
series Kidney & Blood Pressure Research
issn 1420-4096
1423-0143
publishDate 2018-03-01
description Background/Aims: High salt consumption is a major risk factor for hypertension, and sodium homeostasis is regulated by both intestinal sodium absorption and urinary sodium excretion. Chronic caffeine intake has been reported to attenuate salt-sensitive hypertension by promoting urinary sodium excretion; however, its exact role in intestinal sodium absorption remains unknown. Here, we investigated whether and how chronic caffeine consumption antagonizes salt-sensitive hypertension by inhibiting intestinal sodium absorption. Methods: Dahl salt-sensitive rats were fed 8% NaCl chow and 0.1% caffeine in their drinking water for 15 days. The blood pressure and fecal sodium content were measured. The effect of caffeine on the movement of Cl- in enterocyte cells was determined with the Ussing chamber assay. Results: Rats that were treated with caffeine displayed significantly lower mean blood pressure and higher fecal sodium content than the controls. Consistent with these findings, caffeine intake decreased fluid absorption by the intestine in the fluid perfusion experiment. Further, the results from the Ussing chamber assay indicated that caffeine promoted Cl- secretion through enterocyte apical cystic fibrosis transmembrane conductance regulator (CFTR), and thus inhibited sodium absorption. Moreover, depletion of cAMP or inhibition of CFTR completely abolished the effect of caffeine on Cl- secretion. Conclusion: The results indicate that chronic caffeine consumption reduces sodium absorption by promoting CFTR-mediated Cl- secretion in the intestine, which contributes to the anti-hypertensive effect of caffeine in salt-sensitive rats.
topic Caffeine
Salt-sensitive hypertension
Intestinal sodium absorption
CFTR
CAMP
url https://www.karger.com/Article/FullText/488256
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