In vitro evaluation of antimicrobial efficacy of silver zeolite against common oral pathogens
Objectives. Antimicrobial efficacy and toxicity analysis of 5 different concentrations of silver zeolite (SZ) compound against 5 common oral pathogens Streptococcus mutans, Streptococcus pyogenes, Lactobacillus sp., Staphylococcus aureus, Candida albicans. Material and methods. The antimicrobial ef...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
National Institute of Public Health - National Institute of Hygiene
2021-06-01
|
Series: | Roczniki Panstwowego Zakladu Higieny |
Subjects: | |
Online Access: | http://wydawnictwa.pzh.gov.pl/roczniki_pzh/pobierz-artykul?id=1386 |
id |
doaj-0b39dc4fb9a9410a80d4ca6423ec787f |
---|---|
record_format |
Article |
spelling |
doaj-0b39dc4fb9a9410a80d4ca6423ec787f2021-06-17T10:58:18ZengNational Institute of Public Health - National Institute of HygieneRoczniki Panstwowego Zakladu Higieny0035-77152451-23112021-06-0172220320810.32394/rpzh.2021.0161In vitro evaluation of antimicrobial efficacy of silver zeolite against common oral pathogensSangram Panda0Shakti Rath1Mirna Garhanayak2Sourav Chandra Bidyasagar Bal3Ramesh Nagarajappa4Department of Prosthodontics, Institute of Dental Sciences, Siksha ‘O’ Anusandhan (Deemed to be University), Bhubaneswar 751003, Odisha, IndiaCentral Research Laboratory, Institute of Dental Sciences, Siksha ‘O’ Anusandhan (Deemed to be University), Bhubaneswar 751003, Odisha, IndiaDepartment of Prosthodontics, Institute of Dental Sciences, Siksha ‘O’ Anusandhan (Deemed to be University), Bhubaneswar 751003, Odisha, IndiaDepartment of Public Health Dentistry, Institute of Dental Sciences, Siksha ‘O’ Anusandhan (Deemed to be University), Bhubaneswar 751003, Odisha, IndiaDepartment of Public Health Dentistry, Institute of Dental Sciences, Siksha ‘O’ Anusandhan (Deemed to be University), Bhubaneswar 751003, Odisha, IndiaObjectives. Antimicrobial efficacy and toxicity analysis of 5 different concentrations of silver zeolite (SZ) compound against 5 common oral pathogens Streptococcus mutans, Streptococcus pyogenes, Lactobacillus sp., Staphylococcus aureus, Candida albicans. Material and methods. The antimicrobial efficacy of 5 different concentrations of SZ was tested against 5 common oral pathogens using the agar well diffusion method and the MIC and MBC values were determined using the micro broth dilution method. The toxicity of all 5 different concentrations was evaluated using brine shrimp assay and lethal concentration (Lc50) value was determined. Results. At 10 μg/mL the antimicrobial activity of SZ was almost negligible. The antimicrobial activity was observed in an increasing trend against all the test microorganisms as the concentration increased. At 75 & 100 μg/mL the zone of inhibitions was more than the control. Furthermore, MIC and MBC values of SZ with concentrations 25, 50, 75, and 100 μg/mL were determined and recorded. SZ was equally effective against all the test organisms. The LC25 (lethal concentration25) value was 1.6 μg/mL, whereas the LC50 value was 1.77 μg/mL and the LC75 value was 1.90 μg/mL, calculated from the probit computational method. Conclusion. SZ has the potential to change the ongoing system and bring about a revolution as an antimicrobial drug. However, the dose must be regulated as it can be toxic in higher concentrations. SZ compounds with the correct study of physicochemical properties and toxicity analysis can increase their pharmacological use and market value.http://wydawnictwa.pzh.gov.pl/roczniki_pzh/pobierz-artykul?id=1386silver zeoliteoral pathogensantimicrobial efficacytoxicity analysis; pharmaceutical applications |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sangram Panda Shakti Rath Mirna Garhanayak Sourav Chandra Bidyasagar Bal Ramesh Nagarajappa |
spellingShingle |
Sangram Panda Shakti Rath Mirna Garhanayak Sourav Chandra Bidyasagar Bal Ramesh Nagarajappa In vitro evaluation of antimicrobial efficacy of silver zeolite against common oral pathogens Roczniki Panstwowego Zakladu Higieny silver zeolite oral pathogens antimicrobial efficacy toxicity analysis; pharmaceutical applications |
author_facet |
Sangram Panda Shakti Rath Mirna Garhanayak Sourav Chandra Bidyasagar Bal Ramesh Nagarajappa |
author_sort |
Sangram Panda |
title |
In vitro evaluation of antimicrobial efficacy of silver zeolite against common oral pathogens |
title_short |
In vitro evaluation of antimicrobial efficacy of silver zeolite against common oral pathogens |
title_full |
In vitro evaluation of antimicrobial efficacy of silver zeolite against common oral pathogens |
title_fullStr |
In vitro evaluation of antimicrobial efficacy of silver zeolite against common oral pathogens |
title_full_unstemmed |
In vitro evaluation of antimicrobial efficacy of silver zeolite against common oral pathogens |
title_sort |
in vitro evaluation of antimicrobial efficacy of silver zeolite against common oral pathogens |
publisher |
National Institute of Public Health - National Institute of Hygiene |
series |
Roczniki Panstwowego Zakladu Higieny |
issn |
0035-7715 2451-2311 |
publishDate |
2021-06-01 |
description |
Objectives. Antimicrobial efficacy and toxicity analysis of 5 different concentrations of silver zeolite (SZ) compound against 5 common oral pathogens Streptococcus mutans, Streptococcus pyogenes, Lactobacillus sp., Staphylococcus aureus, Candida albicans.
Material and methods. The antimicrobial efficacy of 5 different concentrations of SZ was tested against 5 common oral pathogens using the agar well diffusion method and the MIC and MBC values were determined using the micro broth dilution method. The toxicity of all 5 different concentrations was evaluated using brine shrimp assay and lethal concentration (Lc50) value was determined.
Results. At 10 μg/mL the antimicrobial activity of SZ was almost negligible. The antimicrobial activity was observed in an increasing trend against all the test microorganisms as the concentration increased. At 75 & 100 μg/mL the zone of inhibitions was more than the control. Furthermore, MIC and MBC values of SZ with concentrations 25, 50, 75, and 100 μg/mL were determined and recorded. SZ was equally effective against all the test organisms. The LC25 (lethal concentration25) value was 1.6 μg/mL, whereas the LC50 value was 1.77 μg/mL and the LC75 value was 1.90 μg/mL, calculated from the probit computational method.
Conclusion. SZ has the potential to change the ongoing system and bring about a revolution as an antimicrobial drug.
However, the dose must be regulated as it can be toxic in higher concentrations. SZ compounds with the correct study of physicochemical properties and toxicity analysis can increase their pharmacological use and market value. |
topic |
silver zeolite oral pathogens antimicrobial efficacy toxicity analysis; pharmaceutical applications |
url |
http://wydawnictwa.pzh.gov.pl/roczniki_pzh/pobierz-artykul?id=1386 |
work_keys_str_mv |
AT sangrampanda invitroevaluationofantimicrobialefficacyofsilverzeoliteagainstcommonoralpathogens AT shaktirath invitroevaluationofantimicrobialefficacyofsilverzeoliteagainstcommonoralpathogens AT mirnagarhanayak invitroevaluationofantimicrobialefficacyofsilverzeoliteagainstcommonoralpathogens AT souravchandrabidyasagarbal invitroevaluationofantimicrobialefficacyofsilverzeoliteagainstcommonoralpathogens AT rameshnagarajappa invitroevaluationofantimicrobialefficacyofsilverzeoliteagainstcommonoralpathogens |
_version_ |
1721374024745877504 |