Variations in glutamine deamidation for a Châtelperronian bone assemblage as measured by peptide mass fingerprinting of collagen
Peptide mass fingerprinting of bone collagen (ZooMS) has previously been proposed as a method to calculate the extent of the non-enzymatic degradation of glutamine into glutamic acid (deamidation). Temporal and spatial variation of glutamine deamidation at a single site, however, has not been invest...
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doaj-0b413ee7ea68424a8734b1dfb08462432020-11-24T22:16:36ZengTaylor & Francis GroupScience and Technology of Archaeological Research2054-89232017-01-0131152710.1080/20548923.2016.12588251258825Variations in glutamine deamidation for a Châtelperronian bone assemblage as measured by peptide mass fingerprinting of collagenFrido Welker0Marie A. Soressi1Morgan Roussel2Isolde van Riemsdijk3Jean-Jacques Hublin4Matthew J. Collins5Max Planck Institute for Evolutionary AnthropologyMax Planck Institute for Evolutionary AnthropologyLeiden UniversityWageningen UniversityMax Planck Institute for Evolutionary AnthropologyBioArCh, University of YorkPeptide mass fingerprinting of bone collagen (ZooMS) has previously been proposed as a method to calculate the extent of the non-enzymatic degradation of glutamine into glutamic acid (deamidation). Temporal and spatial variation of glutamine deamidation at a single site, however, has not been investigated. Here we apply ZooMS screening of Châtelperronian and Early Holocene bone specimens from Quinçay, France, to explore temporal and spatial variation in glutamine deamidation. Our results indicate that chronological resolution is low, while spatial variation is high. Nevertheless, our analysis allows the identification of bone specimens that have undergone diagenetic histories remarkably different (either in length or in type) from spatially related bone specimens. Therefore, ZooMS ammonium-bicarbonate screening is capable of testing bone assemblage homogeneity, which could guide subsequent analysis and interpretation.http://dx.doi.org/10.1080/20548923.2016.1258825ZooMSChâtelperronianDiagenesisQuinçayGlutamine DeamidationBone |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Frido Welker Marie A. Soressi Morgan Roussel Isolde van Riemsdijk Jean-Jacques Hublin Matthew J. Collins |
spellingShingle |
Frido Welker Marie A. Soressi Morgan Roussel Isolde van Riemsdijk Jean-Jacques Hublin Matthew J. Collins Variations in glutamine deamidation for a Châtelperronian bone assemblage as measured by peptide mass fingerprinting of collagen Science and Technology of Archaeological Research ZooMS Châtelperronian Diagenesis Quinçay Glutamine Deamidation Bone |
author_facet |
Frido Welker Marie A. Soressi Morgan Roussel Isolde van Riemsdijk Jean-Jacques Hublin Matthew J. Collins |
author_sort |
Frido Welker |
title |
Variations in glutamine deamidation for a Châtelperronian bone assemblage as measured by peptide mass fingerprinting of collagen |
title_short |
Variations in glutamine deamidation for a Châtelperronian bone assemblage as measured by peptide mass fingerprinting of collagen |
title_full |
Variations in glutamine deamidation for a Châtelperronian bone assemblage as measured by peptide mass fingerprinting of collagen |
title_fullStr |
Variations in glutamine deamidation for a Châtelperronian bone assemblage as measured by peptide mass fingerprinting of collagen |
title_full_unstemmed |
Variations in glutamine deamidation for a Châtelperronian bone assemblage as measured by peptide mass fingerprinting of collagen |
title_sort |
variations in glutamine deamidation for a châtelperronian bone assemblage as measured by peptide mass fingerprinting of collagen |
publisher |
Taylor & Francis Group |
series |
Science and Technology of Archaeological Research |
issn |
2054-8923 |
publishDate |
2017-01-01 |
description |
Peptide mass fingerprinting of bone collagen (ZooMS) has previously been proposed as a method to calculate the extent of the non-enzymatic degradation of glutamine into glutamic acid (deamidation). Temporal and spatial variation of glutamine deamidation at a single site, however, has not been investigated. Here we apply ZooMS screening of Châtelperronian and Early Holocene bone specimens from Quinçay, France, to explore temporal and spatial variation in glutamine deamidation. Our results indicate that chronological resolution is low, while spatial variation is high. Nevertheless, our analysis allows the identification of bone specimens that have undergone diagenetic histories remarkably different (either in length or in type) from spatially related bone specimens. Therefore, ZooMS ammonium-bicarbonate screening is capable of testing bone assemblage homogeneity, which could guide subsequent analysis and interpretation. |
topic |
ZooMS Châtelperronian Diagenesis Quinçay Glutamine Deamidation Bone |
url |
http://dx.doi.org/10.1080/20548923.2016.1258825 |
work_keys_str_mv |
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