Elaboration of indirect method (test) for determination of enantiomeric purity of chiral PANAM (polyamide nucleic acids mimetics) monomers

Elaboration of indirect method (test) for determination of enantiomeric purity of chiral PANAM (polyamide nucleic acids mimetics) monomers

An indirect approach for the determination of enantiomeric purity of chiral α-(benzyl)-carboxyethyl- and γ-methyl-substituted monomers of polyamide nucleic acids mimics (PANAM) is described. The test consist in the synthesis of C-terminal diastereomers with methyl ester of L- or DL-isoleucine for ea...

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Main Authors: Yu. G. Kirillova, A. V. Ryakin, D. I. Prokhorov, M. A. Lyanov, O. V. Esipova, S. V. Eremin, E. E. Zvonkova
Format: Article
Language:Russian
Published: MIREA - Russian Technological University 2012-04-01
Series:Тонкие химические технологии
Subjects:
peptide nucleic acids (pna)
polyamide nucleic acids mimics (panam)
chiral monomers
enantiomeric purity
reverse phase hplc
<sup><i>1</sup>н-nmr-spectroscopy
Online Access:https://www.finechem-mirea.ru/jour/article/view/758
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spelling doaj-0b5540ee7a454ad397727e04161362392021-07-28T13:23:55ZrusMIREA - Russian Technological UniversityТонкие химические технологии2410-65932686-75752012-04-01727074752Elaboration of indirect method (test) for determination of enantiomeric purity of chiral PANAM (polyamide nucleic acids mimetics) monomersYu. G. Kirillova0A. V. Ryakin1D. I. Prokhorov2M. A. Lyanov3O. V. Esipova4S. V. Eremin5E. E. Zvonkova6M.V. Lomonosov Moscow State University of Fine Chemical Technologies, 86, Vernadskogo pr., Moscow 119571M.V. Lomonosov Moscow State University of Fine Chemical Technologies, 86, Vernadskogo pr., Moscow 119571M.V. Lomonosov Moscow State University of Fine Chemical Technologies, 86, Vernadskogo pr., Moscow 119571M.V. Lomonosov Moscow State University of Fine Chemical Technologies, 86, Vernadskogo pr., Moscow 119571M.V. Lomonosov Moscow State University of Fine Chemical Technologies, 86, Vernadskogo pr., Moscow 119571M.V. Lomonosov Moscow State University of Fine Chemical Technologies, 86, Vernadskogo pr., Moscow 119571M.V. Lomonosov Moscow State University of Fine Chemical Technologies, 86, Vernadskogo pr., Moscow 119571An indirect approach for the determination of enantiomeric purity of chiral α-(benzyl)-carboxyethyl- and γ-methyl-substituted monomers of polyamide nucleic acids mimics (PANAM) is described. The test consist in the synthesis of C-terminal diastereomers with methyl ester of L- or DL-isoleucine for each PANAM monomer and estimation of the relative content (abundance ratio) of main and minor diastereomers by means of reverse phase HPLC and 1H-NMR spectroscopy.https://www.finechem-mirea.ru/jour/article/view/758peptide nucleic acids (pna)polyamide nucleic acids mimics (panam)chiral monomersenantiomeric purityreverse phase hplc<sup><i>1</sup>н-nmr-spectroscopy
collection DOAJ
language Russian
format Article
sources DOAJ
author Yu. G. Kirillova
A. V. Ryakin
D. I. Prokhorov
M. A. Lyanov
O. V. Esipova
S. V. Eremin
E. E. Zvonkova
spellingShingle Yu. G. Kirillova
A. V. Ryakin
D. I. Prokhorov
M. A. Lyanov
O. V. Esipova
S. V. Eremin
E. E. Zvonkova
Elaboration of indirect method (test) for determination of enantiomeric purity of chiral PANAM (polyamide nucleic acids mimetics) monomers
Тонкие химические технологии
peptide nucleic acids (pna)
polyamide nucleic acids mimics (panam)
chiral monomers
enantiomeric purity
reverse phase hplc
<sup><i>1</sup>н-nmr-spectroscopy
author_facet Yu. G. Kirillova
A. V. Ryakin
D. I. Prokhorov
M. A. Lyanov
O. V. Esipova
S. V. Eremin
E. E. Zvonkova
author_sort Yu. G. Kirillova
title Elaboration of indirect method (test) for determination of enantiomeric purity of chiral PANAM (polyamide nucleic acids mimetics) monomers
title_short Elaboration of indirect method (test) for determination of enantiomeric purity of chiral PANAM (polyamide nucleic acids mimetics) monomers
title_full Elaboration of indirect method (test) for determination of enantiomeric purity of chiral PANAM (polyamide nucleic acids mimetics) monomers
title_fullStr Elaboration of indirect method (test) for determination of enantiomeric purity of chiral PANAM (polyamide nucleic acids mimetics) monomers
title_full_unstemmed Elaboration of indirect method (test) for determination of enantiomeric purity of chiral PANAM (polyamide nucleic acids mimetics) monomers
title_sort elaboration of indirect method (test) for determination of enantiomeric purity of chiral panam (polyamide nucleic acids mimetics) monomers
publisher MIREA - Russian Technological University
series Тонкие химические технологии
issn 2410-6593
2686-7575
publishDate 2012-04-01
description An indirect approach for the determination of enantiomeric purity of chiral α-(benzyl)-carboxyethyl- and γ-methyl-substituted monomers of polyamide nucleic acids mimics (PANAM) is described. The test consist in the synthesis of C-terminal diastereomers with methyl ester of L- or DL-isoleucine for each PANAM monomer and estimation of the relative content (abundance ratio) of main and minor diastereomers by means of reverse phase HPLC and 1H-NMR spectroscopy.
topic peptide nucleic acids (pna)
polyamide nucleic acids mimics (panam)
chiral monomers
enantiomeric purity
reverse phase hplc
<sup><i>1</sup>н-nmr-spectroscopy
url https://www.finechem-mirea.ru/jour/article/view/758
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AT avryakin elaborationofindirectmethodtestfordeterminationofenantiomericpurityofchiralpanampolyamidenucleicacidsmimeticsmonomers
AT diprokhorov elaborationofindirectmethodtestfordeterminationofenantiomericpurityofchiralpanampolyamidenucleicacidsmimeticsmonomers
AT malyanov elaborationofindirectmethodtestfordeterminationofenantiomericpurityofchiralpanampolyamidenucleicacidsmimeticsmonomers
AT ovesipova elaborationofindirectmethodtestfordeterminationofenantiomericpurityofchiralpanampolyamidenucleicacidsmimeticsmonomers
AT sveremin elaborationofindirectmethodtestfordeterminationofenantiomericpurityofchiralpanampolyamidenucleicacidsmimeticsmonomers
AT eezvonkova elaborationofindirectmethodtestfordeterminationofenantiomericpurityofchiralpanampolyamidenucleicacidsmimeticsmonomers
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