Comparative Analysis of Lipid Peroxidation System in Humans and Rats with Arterial Hypertension

• Main Authors: Marina Darenskaya, Larisa Kolesnikova, Lyubov Rychkova, Lyudmila Grebenkina, Natalya Semenova, Elena Druzhinina, Albina Labygina, Elena Proskurnina, Sergey Kolesnikov, Lyubov Kolesnikova
• Format: Article
• Language: English
• Published: International Medical Research and Development Corporation 2019-12-01
• Series: International Journal of Biomedicine
• Subjects: arterial hypertension; rats; adolescents; lipid peroxidation; antioxidant defense
• Online Access: http://ijbm.org/articles/i36/ijbm_9(4)_oa3.pdf

The aim of this research was to study the concentration of lipid peroxidation (LPO) products and the activity of antioxidant defense (AOD) parameters in ISIAH rats in comparison with a group of adolescents with arterial hypertension (AH). Material and Methods: We conducted the study on young (2.5-3 months) sexually mature male rats of the normotensive line (WAG) (n=20) (intact animals) and hypertensive line (ISIAH) (n=20), weighing 200-220g. The data of 65 adolescents aged 13-17 years with AH (Group 1) were used for the clinical study (the age of adolescents is comparable to the biological age of the experimental animals used). The comparison group consisted of 65 normotensive adolescents of the same age and sex ("copy-pair" type) (Group 2). The plasma level of antioxidant parameters (total antioxidant activity [TTA], SOD activity, α-tocopherol and retinol) and primary/secondary products of LPO (conjugated dienes [CD], ketodienes and conjugated trienes [KD-CT], and thiobarbituric acid reactants [TBARs]) were determined using spectrophotometric and fluorometric methods. Results: We found that the course of LPO reactions in animals and humans was similar, which was expressed by the activation of prooxidant factors and the insufficiency of antioxidant response. Species differences concerned the intensity and number of parameters involved in the pathological process. Thus, in ISIAH rats there was an increase in toxic TBA-active products and a decrease in TTA, α-tocopherol and retinol in comparison with normotensive animals; in AH adolescents there was an increase in the content of intermediate–KD-CT and final TBA-active products, and a decrease in the α-tocopherol level in relation to the comparison group. Conclusion: Features of response of the LPO nonspecific system in hypertensive rats and humans allow using this line of rats for further study of adaptive mechanisms, and to extrapolate the received experimental data on humans, taking into account certain specific distinctions.