Acute and Subchronic Toxicity Assessment of the Hydroalcoholic Extract of Stachys lavandulifolia in Mice
Stachys lavandulifolia is used as the herbal tea and its wide and potent medical effects have been reported for the extract in animal studies. This study aimed to find the safety profile of the extract to find the appropriate doses for further human studies. The aerial parts of the plant were air-dr...
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Tehran University of Medical Sciences
2011-12-01
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doaj-0b714c0ea2fb48049e26c9a90fe758a62020-11-25T03:22:52ZengTehran University of Medical SciencesActa Medica Iranica0044-60251735-96942011-12-014912Acute and Subchronic Toxicity Assessment of the Hydroalcoholic Extract of Stachys lavandulifolia in MiceFaezeh Monji0Hiva Hossein Tehrani1Zahra Halvaei2Sepideh Arbabi Bidgoli3Young Researchers Club (YRC), Islamic Azad University, Pharmaceutical Sciences Branch (IAUPS), Tehran, Iran.Young Researchers Club (YRC), Islamic Azad University, Pharmaceutical Sciences Branch (IAUPS), Tehran, Iran.Young Researchers Club (YRC), Islamic Azad University, Pharmaceutical Sciences Branch (IAUPS), Tehran, Iran.Department of Toxicology & Pharmacology, Islamic Azad University, Pharmaceutical Sciences Branch (IAUPS), Tehran, Iran.Stachys lavandulifolia is used as the herbal tea and its wide and potent medical effects have been reported for the extract in animal studies. This study aimed to find the safety profile of the extract to find the appropriate doses for further human studies. The aerial parts of the plant were air-dried and the hydroalcoholic extract was obtained and concentrated by percolation method with 140 mg/ml concentration. To assess the toxicity profile of this extract, 60 female mice (30 cases, 30 controls, 24.8 ±2.1 g, 4-6 weeks) were administered the extract by oral gavages in acute (24 hrs), subacute (14 days) and subchronic (45 days) models. All clinical, hematological, biochemical and histopathological changes were assessed in appropriate midpoints and endpoints and compared with control group. Doses up to 140 mg⁄kg were recognized as maximum tolerated dose in subchronic model. Abnormal changes in kidney and liver weight in treatment groups as well as the significant elevation of biochemical parameters in 45 days study has suggested the possible hepatic and renal toxicity potentials of this extract with doses upper than 140mg⁄kg. Doses up 70 mg⁄kg could be considered as no observable adverse effect level (NOAEL) and could be used in further clinical trials on the possible therapeutic effects of this plant. https://acta.tums.ac.ir/index.php/acta/article/view/3836Stachys lavandulifoliaAcute toxicitySubacute toxicitySubchronic toxicityNOAEL |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Faezeh Monji Hiva Hossein Tehrani Zahra Halvaei Sepideh Arbabi Bidgoli |
spellingShingle |
Faezeh Monji Hiva Hossein Tehrani Zahra Halvaei Sepideh Arbabi Bidgoli Acute and Subchronic Toxicity Assessment of the Hydroalcoholic Extract of Stachys lavandulifolia in Mice Acta Medica Iranica Stachys lavandulifolia Acute toxicity Subacute toxicity Subchronic toxicity NOAEL |
author_facet |
Faezeh Monji Hiva Hossein Tehrani Zahra Halvaei Sepideh Arbabi Bidgoli |
author_sort |
Faezeh Monji |
title |
Acute and Subchronic Toxicity Assessment of the Hydroalcoholic Extract of Stachys lavandulifolia in Mice |
title_short |
Acute and Subchronic Toxicity Assessment of the Hydroalcoholic Extract of Stachys lavandulifolia in Mice |
title_full |
Acute and Subchronic Toxicity Assessment of the Hydroalcoholic Extract of Stachys lavandulifolia in Mice |
title_fullStr |
Acute and Subchronic Toxicity Assessment of the Hydroalcoholic Extract of Stachys lavandulifolia in Mice |
title_full_unstemmed |
Acute and Subchronic Toxicity Assessment of the Hydroalcoholic Extract of Stachys lavandulifolia in Mice |
title_sort |
acute and subchronic toxicity assessment of the hydroalcoholic extract of stachys lavandulifolia in mice |
publisher |
Tehran University of Medical Sciences |
series |
Acta Medica Iranica |
issn |
0044-6025 1735-9694 |
publishDate |
2011-12-01 |
description |
Stachys lavandulifolia is used as the herbal tea and its wide and potent medical effects have been reported for the extract in animal studies. This study aimed to find the safety profile of the extract to find the appropriate doses for further human studies. The aerial parts of the plant were air-dried and the hydroalcoholic extract was obtained and concentrated by percolation method with 140 mg/ml concentration. To assess the toxicity profile of this extract, 60 female mice (30 cases, 30 controls, 24.8 ±2.1 g, 4-6 weeks) were administered the extract by oral gavages in acute (24 hrs), subacute (14 days) and subchronic (45 days) models. All clinical, hematological, biochemical and histopathological changes were assessed in appropriate midpoints and endpoints and compared with control group. Doses up to 140 mg⁄kg were recognized as maximum tolerated dose in subchronic model. Abnormal changes in kidney and liver weight in treatment groups as well as the significant elevation of biochemical parameters in 45 days study has suggested the possible hepatic and renal toxicity potentials of this extract with doses upper than 140mg⁄kg. Doses up 70 mg⁄kg could be considered as no observable adverse effect level (NOAEL) and could be used in further clinical trials on the possible therapeutic effects of this plant.
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topic |
Stachys lavandulifolia Acute toxicity Subacute toxicity Subchronic toxicity NOAEL |
url |
https://acta.tums.ac.ir/index.php/acta/article/view/3836 |
work_keys_str_mv |
AT faezehmonji acuteandsubchronictoxicityassessmentofthehydroalcoholicextractofstachyslavandulifoliainmice AT hivahosseintehrani acuteandsubchronictoxicityassessmentofthehydroalcoholicextractofstachyslavandulifoliainmice AT zahrahalvaei acuteandsubchronictoxicityassessmentofthehydroalcoholicextractofstachyslavandulifoliainmice AT sepideharbabibidgoli acuteandsubchronictoxicityassessmentofthehydroalcoholicextractofstachyslavandulifoliainmice |
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