Acute and Subchronic Toxicity Assessment of the Hydroalcoholic Extract of Stachys lavandulifolia in Mice

Stachys lavandulifolia is used as the herbal tea and its wide and potent medical effects have been reported for the extract in animal studies. This study aimed to find the safety profile of the extract to find the appropriate doses for further human studies. The aerial parts of the plant were air-dr...

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Main Authors: Faezeh Monji, Hiva Hossein Tehrani, Zahra Halvaei, Sepideh Arbabi Bidgoli
Format: Article
Language:English
Published: Tehran University of Medical Sciences 2011-12-01
Series:Acta Medica Iranica
Subjects:
Online Access:https://acta.tums.ac.ir/index.php/acta/article/view/3836
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spelling doaj-0b714c0ea2fb48049e26c9a90fe758a62020-11-25T03:22:52ZengTehran University of Medical SciencesActa Medica Iranica0044-60251735-96942011-12-014912Acute and Subchronic Toxicity Assessment of the Hydroalcoholic Extract of Stachys lavandulifolia in MiceFaezeh Monji0Hiva Hossein Tehrani1Zahra Halvaei2Sepideh Arbabi Bidgoli3Young Researchers Club (YRC), Islamic Azad University, Pharmaceutical Sciences Branch (IAUPS), Tehran, Iran.Young Researchers Club (YRC), Islamic Azad University, Pharmaceutical Sciences Branch (IAUPS), Tehran, Iran.Young Researchers Club (YRC), Islamic Azad University, Pharmaceutical Sciences Branch (IAUPS), Tehran, Iran.Department of Toxicology & Pharmacology, Islamic Azad University, Pharmaceutical Sciences Branch (IAUPS), Tehran, Iran.Stachys lavandulifolia is used as the herbal tea and its wide and potent medical effects have been reported for the extract in animal studies. This study aimed to find the safety profile of the extract to find the appropriate doses for further human studies. The aerial parts of the plant were air-dried and the hydroalcoholic extract was obtained and concentrated by percolation method with 140 mg/ml concentration. To assess the toxicity profile of this extract, 60 female mice (30 cases, 30 controls, 24.8 ±2.1 g, 4-6 weeks) were administered the extract by oral gavages in acute (24 hrs), subacute (14 days) and subchronic (45 days) models. All clinical, hematological, biochemical and histopathological changes were assessed in appropriate midpoints and endpoints and compared with control group. Doses up to 140 mg⁄kg were recognized as maximum tolerated dose in subchronic model. Abnormal changes in kidney and liver weight in treatment groups as well as the significant elevation of biochemical parameters in 45 days study has suggested the possible hepatic and renal toxicity potentials of this extract with doses upper than 140mg⁄kg. Doses up 70 mg⁄kg could be considered as no observable adverse effect level (NOAEL) and could be used in further clinical trials on the possible therapeutic effects of this plant. https://acta.tums.ac.ir/index.php/acta/article/view/3836Stachys lavandulifoliaAcute toxicitySubacute toxicitySubchronic toxicityNOAEL
collection DOAJ
language English
format Article
sources DOAJ
author Faezeh Monji
Hiva Hossein Tehrani
Zahra Halvaei
Sepideh Arbabi Bidgoli
spellingShingle Faezeh Monji
Hiva Hossein Tehrani
Zahra Halvaei
Sepideh Arbabi Bidgoli
Acute and Subchronic Toxicity Assessment of the Hydroalcoholic Extract of Stachys lavandulifolia in Mice
Acta Medica Iranica
Stachys lavandulifolia
Acute toxicity
Subacute toxicity
Subchronic toxicity
NOAEL
author_facet Faezeh Monji
Hiva Hossein Tehrani
Zahra Halvaei
Sepideh Arbabi Bidgoli
author_sort Faezeh Monji
title Acute and Subchronic Toxicity Assessment of the Hydroalcoholic Extract of Stachys lavandulifolia in Mice
title_short Acute and Subchronic Toxicity Assessment of the Hydroalcoholic Extract of Stachys lavandulifolia in Mice
title_full Acute and Subchronic Toxicity Assessment of the Hydroalcoholic Extract of Stachys lavandulifolia in Mice
title_fullStr Acute and Subchronic Toxicity Assessment of the Hydroalcoholic Extract of Stachys lavandulifolia in Mice
title_full_unstemmed Acute and Subchronic Toxicity Assessment of the Hydroalcoholic Extract of Stachys lavandulifolia in Mice
title_sort acute and subchronic toxicity assessment of the hydroalcoholic extract of stachys lavandulifolia in mice
publisher Tehran University of Medical Sciences
series Acta Medica Iranica
issn 0044-6025
1735-9694
publishDate 2011-12-01
description Stachys lavandulifolia is used as the herbal tea and its wide and potent medical effects have been reported for the extract in animal studies. This study aimed to find the safety profile of the extract to find the appropriate doses for further human studies. The aerial parts of the plant were air-dried and the hydroalcoholic extract was obtained and concentrated by percolation method with 140 mg/ml concentration. To assess the toxicity profile of this extract, 60 female mice (30 cases, 30 controls, 24.8 ±2.1 g, 4-6 weeks) were administered the extract by oral gavages in acute (24 hrs), subacute (14 days) and subchronic (45 days) models. All clinical, hematological, biochemical and histopathological changes were assessed in appropriate midpoints and endpoints and compared with control group. Doses up to 140 mg⁄kg were recognized as maximum tolerated dose in subchronic model. Abnormal changes in kidney and liver weight in treatment groups as well as the significant elevation of biochemical parameters in 45 days study has suggested the possible hepatic and renal toxicity potentials of this extract with doses upper than 140mg⁄kg. Doses up 70 mg⁄kg could be considered as no observable adverse effect level (NOAEL) and could be used in further clinical trials on the possible therapeutic effects of this plant.
topic Stachys lavandulifolia
Acute toxicity
Subacute toxicity
Subchronic toxicity
NOAEL
url https://acta.tums.ac.ir/index.php/acta/article/view/3836
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