Effect of type 2 diabetic serum on the behavior of Wharton's jelly-derived mesenchymal stem cells in vitro
Objective: Wharton jelly-derived mesenchymal stem cells (WJMSCs) exhibit multilineage differentiation potential and can be used to treat multiple organs. However, diabetes affects the repair capability of MSCs. The aim of this study was to evaluate the effect of diabetic patient-derived serum on WJM...
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KeAi Communications Co., Ltd.
2017-06-01
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doaj-0b781e2dfba040d69cb0c164845da6d02021-03-02T10:00:37ZengKeAi Communications Co., Ltd.Chronic Diseases and Translational Medicine2095-882X2017-06-0132105111Effect of type 2 diabetic serum on the behavior of Wharton's jelly-derived mesenchymal stem cells in vitroFatima Ali0Fehmina Aziz1Nadia Wajid2Corresponding author. The University of Lahore, 1-Km, Defence Road, Lahore, Pakistan.; Institute of Molecular Biology and Biotechnology (IMBB) & Centre for Research In Molecular Medicine (CRIMM), The University of Lahore, Raiwind Road, Lahore, PakistanInstitute of Molecular Biology and Biotechnology (IMBB) & Centre for Research In Molecular Medicine (CRIMM), The University of Lahore, Raiwind Road, Lahore, PakistanInstitute of Molecular Biology and Biotechnology (IMBB) & Centre for Research In Molecular Medicine (CRIMM), The University of Lahore, Raiwind Road, Lahore, PakistanObjective: Wharton jelly-derived mesenchymal stem cells (WJMSCs) exhibit multilineage differentiation potential and can be used to treat multiple organs. However, diabetes affects the repair capability of MSCs. The aim of this study was to evaluate the effect of diabetic patient-derived serum on WJMSC behavior. Methods: WJMSCs at passage 3 were treated with serum derived from type 2 diabetic patients. WJMSCs were characterized for surface markers expression by using immunocytochemistry technique. The effects on cell viability, proliferation, cell death rate, and vascular endothelial growth factor level were assessed by crystal violet staining, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay, lactate dehydrogenase assay, and enzyme-linked immuno-sorbent assay, respectively. Oxidative stress was assessed by the estimation of free radical species malondialdehyde (MDA) and enzymes glutathione (GSH), catalase, and superoxide dismutase (SOD). Results: WJMSCs isolated in this study were positive for CD29, CD49, CD73, CD90, CD105, and SSEA4 and negative for CD45 and CD34. Under diabetic stress conditions, WJMSCs showed low viability and high lactate dehydrogenase release. A low level of vascular endothelial growth factor was also observed after diabetic serum treatment. Antioxidant level was also lower in diabetic serum-treated WJMSCs compared to in normal serum-treated WJMSCs. Conclusion: The results of the present study suggest that pre-treatment of WJMSCs with type 2 diabetic serum decreases the survival of WJMSCs. The findings of this study provide insight into diabetes-induced harmful effects on WJMSCs. Keywords: Wharton jelly-derived mesenchymal stem cells, Type 2 diabetes mellitus, Oxidative stress, Vascular endothelial growth factorhttp://www.sciencedirect.com/science/article/pii/S2095882X16301037 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fatima Ali Fehmina Aziz Nadia Wajid |
spellingShingle |
Fatima Ali Fehmina Aziz Nadia Wajid Effect of type 2 diabetic serum on the behavior of Wharton's jelly-derived mesenchymal stem cells in vitro Chronic Diseases and Translational Medicine |
author_facet |
Fatima Ali Fehmina Aziz Nadia Wajid |
author_sort |
Fatima Ali |
title |
Effect of type 2 diabetic serum on the behavior of Wharton's jelly-derived mesenchymal stem cells in vitro |
title_short |
Effect of type 2 diabetic serum on the behavior of Wharton's jelly-derived mesenchymal stem cells in vitro |
title_full |
Effect of type 2 diabetic serum on the behavior of Wharton's jelly-derived mesenchymal stem cells in vitro |
title_fullStr |
Effect of type 2 diabetic serum on the behavior of Wharton's jelly-derived mesenchymal stem cells in vitro |
title_full_unstemmed |
Effect of type 2 diabetic serum on the behavior of Wharton's jelly-derived mesenchymal stem cells in vitro |
title_sort |
effect of type 2 diabetic serum on the behavior of wharton's jelly-derived mesenchymal stem cells in vitro |
publisher |
KeAi Communications Co., Ltd. |
series |
Chronic Diseases and Translational Medicine |
issn |
2095-882X |
publishDate |
2017-06-01 |
description |
Objective: Wharton jelly-derived mesenchymal stem cells (WJMSCs) exhibit multilineage differentiation potential and can be used to treat multiple organs. However, diabetes affects the repair capability of MSCs. The aim of this study was to evaluate the effect of diabetic patient-derived serum on WJMSC behavior. Methods: WJMSCs at passage 3 were treated with serum derived from type 2 diabetic patients. WJMSCs were characterized for surface markers expression by using immunocytochemistry technique. The effects on cell viability, proliferation, cell death rate, and vascular endothelial growth factor level were assessed by crystal violet staining, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay, lactate dehydrogenase assay, and enzyme-linked immuno-sorbent assay, respectively. Oxidative stress was assessed by the estimation of free radical species malondialdehyde (MDA) and enzymes glutathione (GSH), catalase, and superoxide dismutase (SOD). Results: WJMSCs isolated in this study were positive for CD29, CD49, CD73, CD90, CD105, and SSEA4 and negative for CD45 and CD34. Under diabetic stress conditions, WJMSCs showed low viability and high lactate dehydrogenase release. A low level of vascular endothelial growth factor was also observed after diabetic serum treatment. Antioxidant level was also lower in diabetic serum-treated WJMSCs compared to in normal serum-treated WJMSCs. Conclusion: The results of the present study suggest that pre-treatment of WJMSCs with type 2 diabetic serum decreases the survival of WJMSCs. The findings of this study provide insight into diabetes-induced harmful effects on WJMSCs. Keywords: Wharton jelly-derived mesenchymal stem cells, Type 2 diabetes mellitus, Oxidative stress, Vascular endothelial growth factor |
url |
http://www.sciencedirect.com/science/article/pii/S2095882X16301037 |
work_keys_str_mv |
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