Bone Marrow-Derived Mesenchymal Stem Cell Potential Regression of Dysplasia Associating Experimental Liver Fibrosis in Albino Rats
Objectives. Assessing the therapeutic efficacy of superparamagnetic iron oxide nanoparticles (SPIO) labeled bone marrow-derived mesenchymal stem cells (BM-MSCs) on experimental liver fibrosis and associated dysplasia. Materials and Methods. MSCs were obtained from 10 male Sprague-Dawley rats while 5...
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doaj-0b8d536f49154658a1013a04a73553172020-11-25T01:26:22ZengHindawi LimitedBioMed Research International2314-61332314-61412019-01-01201910.1155/2019/53761655376165Bone Marrow-Derived Mesenchymal Stem Cell Potential Regression of Dysplasia Associating Experimental Liver Fibrosis in Albino RatsYasmine H. Khalifa0Ghada M. Mourad1Wahid M. Stephanos2Sahar A. Omar3Radwa A. Mehanna4Histology and Cell Biology Department, Faculty of Medicine, Alexandria University, Dr. Fahmi Abdel Meguid Street, Mowassat Building, El Shatby, Alexandria 21561, EgyptHistology and Cell Biology Department, Faculty of Medicine, Alexandria University, Dr. Fahmi Abdel Meguid Street, Mowassat Building, El Shatby, Alexandria 21561, EgyptHistology and Cell Biology Department, Faculty of Medicine, Alexandria University, Dr. Fahmi Abdel Meguid Street, Mowassat Building, El Shatby, Alexandria 21561, EgyptHistology and Cell Biology Department, Faculty of Medicine, Alexandria University, Dr. Fahmi Abdel Meguid Street, Mowassat Building, El Shatby, Alexandria 21561, EgyptCenter of Excellence for Research in Regenerative Medicine and Applications (CERRMA), Faculty of Medicine, Alexandria University, Alexandria 21514, EgyptObjectives. Assessing the therapeutic efficacy of superparamagnetic iron oxide nanoparticles (SPIO) labeled bone marrow-derived mesenchymal stem cells (BM-MSCs) on experimental liver fibrosis and associated dysplasia. Materials and Methods. MSCs were obtained from 10 male Sprague-Dawley rats while 50 female rats were divided into control (CG), liver fibrosis (CCL4, intraperitoneal injection of CCl4 for 8 weeks), and CCL4 rats treated with SPIO-labeled MSCs (MSCs/CCl4) with and without continuing CCL4 injection for another 8 weeks. Assessment included liver histopathology, liver function tests, transmission electron microscopic tracing for homing of SPIO-MSCs, immunofluorescence histochemistry for fibrosis and dysplasia markers (transforming growth factor-beta (TGF-β1), proliferation nuclear antigen (PCNA), glypican 3)), and quantitative gene expression analysis for matrix metalloproteinase-1 (MMP-1) and tissue inhibitor of metalloproteinase-1 (TIMP-1). Results. SPIO-labeled MSCs were engrafted in the fibrotic liver and the BM/MSCs demonstrated regression for fibrous tissue deposition and inhibition progression of dysplastic changes in the liver of CCl4-treated rats on both the histological and molecular levels. Conclusion. BM-MSCs possess regenerative and antidysplastic potentials.http://dx.doi.org/10.1155/2019/5376165 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yasmine H. Khalifa Ghada M. Mourad Wahid M. Stephanos Sahar A. Omar Radwa A. Mehanna |
spellingShingle |
Yasmine H. Khalifa Ghada M. Mourad Wahid M. Stephanos Sahar A. Omar Radwa A. Mehanna Bone Marrow-Derived Mesenchymal Stem Cell Potential Regression of Dysplasia Associating Experimental Liver Fibrosis in Albino Rats BioMed Research International |
author_facet |
Yasmine H. Khalifa Ghada M. Mourad Wahid M. Stephanos Sahar A. Omar Radwa A. Mehanna |
author_sort |
Yasmine H. Khalifa |
title |
Bone Marrow-Derived Mesenchymal Stem Cell Potential Regression of Dysplasia Associating Experimental Liver Fibrosis in Albino Rats |
title_short |
Bone Marrow-Derived Mesenchymal Stem Cell Potential Regression of Dysplasia Associating Experimental Liver Fibrosis in Albino Rats |
title_full |
Bone Marrow-Derived Mesenchymal Stem Cell Potential Regression of Dysplasia Associating Experimental Liver Fibrosis in Albino Rats |
title_fullStr |
Bone Marrow-Derived Mesenchymal Stem Cell Potential Regression of Dysplasia Associating Experimental Liver Fibrosis in Albino Rats |
title_full_unstemmed |
Bone Marrow-Derived Mesenchymal Stem Cell Potential Regression of Dysplasia Associating Experimental Liver Fibrosis in Albino Rats |
title_sort |
bone marrow-derived mesenchymal stem cell potential regression of dysplasia associating experimental liver fibrosis in albino rats |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2019-01-01 |
description |
Objectives. Assessing the therapeutic efficacy of superparamagnetic iron oxide nanoparticles (SPIO) labeled bone marrow-derived mesenchymal stem cells (BM-MSCs) on experimental liver fibrosis and associated dysplasia. Materials and Methods. MSCs were obtained from 10 male Sprague-Dawley rats while 50 female rats were divided into control (CG), liver fibrosis (CCL4, intraperitoneal injection of CCl4 for 8 weeks), and CCL4 rats treated with SPIO-labeled MSCs (MSCs/CCl4) with and without continuing CCL4 injection for another 8 weeks. Assessment included liver histopathology, liver function tests, transmission electron microscopic tracing for homing of SPIO-MSCs, immunofluorescence histochemistry for fibrosis and dysplasia markers (transforming growth factor-beta (TGF-β1), proliferation nuclear antigen (PCNA), glypican 3)), and quantitative gene expression analysis for matrix metalloproteinase-1 (MMP-1) and tissue inhibitor of metalloproteinase-1 (TIMP-1). Results. SPIO-labeled MSCs were engrafted in the fibrotic liver and the BM/MSCs demonstrated regression for fibrous tissue deposition and inhibition progression of dysplastic changes in the liver of CCl4-treated rats on both the histological and molecular levels. Conclusion. BM-MSCs possess regenerative and antidysplastic potentials. |
url |
http://dx.doi.org/10.1155/2019/5376165 |
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