Cornu cervi pantotrichum Pharmacopuncture Solution Facilitate Hair Growth in C57BL/6 Mice
Objectives: Cornu cervi pantotrichum (CCP) has been widely used in Korean and China, as an anti-fatigue, anti-aging, and tonic agent to enhance the functions of the reproductive and the immune systems. Because CCP has various growth factors that play important roles in the development of hair follic...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Korean Pharmacopuncture Institute
2016-06-01
|
Series: | Journal of Pharmacopuncture |
Subjects: | |
Online Access: | http://dx.doi.org/10.3831/KPI.2016.19.013 |
id |
doaj-0ba90644a3d44e51be09cb1ecdcd6e86 |
---|---|
record_format |
Article |
spelling |
doaj-0ba90644a3d44e51be09cb1ecdcd6e862020-11-25T02:19:28ZengKorean Pharmacopuncture InstituteJournal of Pharmacopuncture2093-69662234-68562016-06-0119212212810.3831/KPI.2016.19.013DHOCBS_2016_v19n2_122Cornu cervi pantotrichum Pharmacopuncture Solution Facilitate Hair Growth in C57BL/6 MiceSeon-Yong Lee0Dong-Jin Lee1Kang Kwon2Chang-Hyun Lee3Hyun Jong Shin4Jai Eun Kim5Ki-Tae Ha6Han-Sol Jeong7Hyung-Sik Seo8Apple Korean Medical Clinic, Seoul, KoreaDepartment of Ophthalmology, Otolaryngology and Dermatology, Korean Medicine Hospital, Pusan National University, Yangsan, KoreaDepartment of Ophthalmology, Otolaryngology and Dermatology, Korean Medicine Hospital, Pusan National University, Yangsan, KoreaDepartment of Anatomy, College of Korean Medicine, Woosuk University, Jeonju, KoreaDepartment of Pathology, College of Korean Medicine, Woosuk Univeristy, Jeonju, KoreaDepartment of Pathology, College of Korean Medicine, Dongguk University, Goyang, KoreaDivision of Applied Medicine, School of Korean Medicine, Pusan National University, Yangsan, KoreaDivision of Applied Medicine, School of Korean Medicine, Pusan National University, Yangsan, KoreaDepartment of Ophthalmology, Otolaryngology and Dermatology, Korean Medicine Hospital, Pusan National University, Yangsan, KoreaObjectives: Cornu cervi pantotrichum (CCP) has been widely used in Korean and China, as an anti-fatigue, anti-aging, and tonic agent to enhance the functions of the reproductive and the immune systems. Because CCP has various growth factors that play important roles in the development of hair follicles, we examined whether CCP pharmacopuncture solution (CCPPS) was capable of promoting hair growth in an animal model. Methods: One day after hair depilation, CCPPS were topically applied to the dorsal skin of C57BL/6 mice once a day for 15 days. Hair growth activity was evaluated by using macro- and microscopic observations. Dorsal skin tissues were stained with hematoxylin and eosin. Expressions of bromodeoxyuridine (BrdU), proliferating cell nuclear antigen (PCNA), and fibroblast growth factor (FGF)-7 were examined by using immunohistochemical staining. A reverse transcription polymerase chain reaction (RT-PCR) analysis was also conducted to measure the messenger RNA (mRNA) expression of FGF-7. Results: CCPPS induced more active hair growth than normal saline. Histologic analysis showed enlargement of the dermal papilla, elongation of the hair shaft, and expansion of hair thickness in CCPPS treated mice, indicating that CCPPS effectively induced the development of anagen. CCPPS treatment markedly increased the expressions of BrdU and PCNA in the hair follicles of C57BL/6 mice. In addition, CCPPS up regulated the expression of FGF-7, which plays an important role in the development of hair follicles. Conclusion: These results reveal that CCPPS facilitates hair re-growth by proliferation of hair follicular cells and up-regulation of FGF-7 and suggest that CCPPS can potentially be applied as an alternative treatment for patients with alopecia.http://dx.doi.org/10.3831/KPI.2016.19.013anagenCornu cervi pantotrichum pharmacopuncture solutionfibroblast growth factor-7hairhair lossproliferating cell nuclear antigen |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Seon-Yong Lee Dong-Jin Lee Kang Kwon Chang-Hyun Lee Hyun Jong Shin Jai Eun Kim Ki-Tae Ha Han-Sol Jeong Hyung-Sik Seo |
spellingShingle |
Seon-Yong Lee Dong-Jin Lee Kang Kwon Chang-Hyun Lee Hyun Jong Shin Jai Eun Kim Ki-Tae Ha Han-Sol Jeong Hyung-Sik Seo Cornu cervi pantotrichum Pharmacopuncture Solution Facilitate Hair Growth in C57BL/6 Mice Journal of Pharmacopuncture anagen Cornu cervi pantotrichum pharmacopuncture solution fibroblast growth factor-7 hair hair loss proliferating cell nuclear antigen |
author_facet |
Seon-Yong Lee Dong-Jin Lee Kang Kwon Chang-Hyun Lee Hyun Jong Shin Jai Eun Kim Ki-Tae Ha Han-Sol Jeong Hyung-Sik Seo |
author_sort |
Seon-Yong Lee |
title |
Cornu cervi pantotrichum Pharmacopuncture Solution Facilitate Hair Growth in C57BL/6 Mice |
title_short |
Cornu cervi pantotrichum Pharmacopuncture Solution Facilitate Hair Growth in C57BL/6 Mice |
title_full |
Cornu cervi pantotrichum Pharmacopuncture Solution Facilitate Hair Growth in C57BL/6 Mice |
title_fullStr |
Cornu cervi pantotrichum Pharmacopuncture Solution Facilitate Hair Growth in C57BL/6 Mice |
title_full_unstemmed |
Cornu cervi pantotrichum Pharmacopuncture Solution Facilitate Hair Growth in C57BL/6 Mice |
title_sort |
cornu cervi pantotrichum pharmacopuncture solution facilitate hair growth in c57bl/6 mice |
publisher |
Korean Pharmacopuncture Institute |
series |
Journal of Pharmacopuncture |
issn |
2093-6966 2234-6856 |
publishDate |
2016-06-01 |
description |
Objectives: Cornu cervi pantotrichum (CCP) has been widely used in Korean and China, as an anti-fatigue, anti-aging, and tonic agent to enhance the functions of the reproductive and the immune systems. Because CCP has various growth factors that play important roles in the development of hair follicles, we examined whether CCP pharmacopuncture solution (CCPPS) was capable of promoting hair growth in an animal model. Methods: One day after hair depilation, CCPPS were topically applied to the dorsal skin of C57BL/6 mice once a day for 15 days. Hair growth activity was evaluated by using macro- and microscopic observations. Dorsal skin tissues were stained with hematoxylin and eosin. Expressions of bromodeoxyuridine (BrdU), proliferating cell nuclear antigen (PCNA), and fibroblast growth factor (FGF)-7 were examined by using immunohistochemical staining. A reverse transcription polymerase chain reaction (RT-PCR) analysis was also conducted to measure the messenger RNA (mRNA) expression of FGF-7. Results: CCPPS induced more active hair growth than normal saline. Histologic analysis showed enlargement of the dermal papilla, elongation of the hair shaft, and expansion of hair thickness in CCPPS treated mice, indicating that CCPPS effectively induced the development of anagen. CCPPS treatment markedly increased the expressions of BrdU and PCNA in the hair follicles of C57BL/6 mice. In addition, CCPPS up regulated the expression of FGF-7, which plays an important role in the development of hair follicles. Conclusion: These results reveal that CCPPS facilitates hair re-growth by proliferation of hair follicular cells and up-regulation of FGF-7 and suggest that CCPPS can potentially be applied as an alternative treatment for patients with alopecia. |
topic |
anagen Cornu cervi pantotrichum pharmacopuncture solution fibroblast growth factor-7 hair hair loss proliferating cell nuclear antigen |
url |
http://dx.doi.org/10.3831/KPI.2016.19.013 |
work_keys_str_mv |
AT seonyonglee cornucervipantotrichumpharmacopuncturesolutionfacilitatehairgrowthinc57bl6mice AT dongjinlee cornucervipantotrichumpharmacopuncturesolutionfacilitatehairgrowthinc57bl6mice AT kangkwon cornucervipantotrichumpharmacopuncturesolutionfacilitatehairgrowthinc57bl6mice AT changhyunlee cornucervipantotrichumpharmacopuncturesolutionfacilitatehairgrowthinc57bl6mice AT hyunjongshin cornucervipantotrichumpharmacopuncturesolutionfacilitatehairgrowthinc57bl6mice AT jaieunkim cornucervipantotrichumpharmacopuncturesolutionfacilitatehairgrowthinc57bl6mice AT kitaeha cornucervipantotrichumpharmacopuncturesolutionfacilitatehairgrowthinc57bl6mice AT hansoljeong cornucervipantotrichumpharmacopuncturesolutionfacilitatehairgrowthinc57bl6mice AT hyungsikseo cornucervipantotrichumpharmacopuncturesolutionfacilitatehairgrowthinc57bl6mice |
_version_ |
1724876703128354816 |