Biosurfactant production and hydrocarbon degradation activity of endophytic bacteria isolated from Chelidonium majus L.

• Main Authors: Olga Marchut-Mikolajczyk, Piotr Drożdżyński, Dominika Pietrzyk, Tadeusz Antczak
• Format: Article
• Language: English
• Published: BMC 2018-11-01
• Series: Microbial Cell Factories
• Subjects: Endophytes; Biosurfactant; Hydrocarbons; Degradation; Plant growth-promoting agent
• Online Access: http://link.springer.com/article/10.1186/s12934-018-1017-5

Abstract Background The process of plant growth in the contaminated environment is often inhibited and entails the neutralization of harmful compounds. To reduce the negative impact of harmful compounds microorganisms produce unique compounds called biosurfactants. This paper describes the potential of culturable endophytic microorganisms from synanthropic plant-Chelidonium majus L. for the production of biosurfactants, as indirect plant promoting factors as well as their degradation activity. Emulsifying activity and degradation potential of tested strains were assessed by cultivation of isolates in the presence of diesel oil and waste engine oil. Results Ten bacterial strain were isolated. Analysis of emulsifying activity revealed that all isolates possessed the ability for biosurfactant production. However, one of the isolated endophytes—2A, identified as Bacillus pumilus, exhibited the highest emulsifying activity (OD500 1.96). The same strain has shown very high degradation potential, both for diesel oil and waste engine oil hydrocarbons. Results obtained with the Phytotoxkit tests revealed that the addition of biosurfactant isolated from B. pumilus 2A strain resulted in stimulation of seed germination in soil contaminated with diesel oil (137%) and waste engine oil (120%). Positive impact of the biosurfactant produced by B. pumilus 2A on the growth of Sinapis alba in hydrocarbons contaminated soil was demonstrated. Conclusions The endophytic strain identified as Bacillus pumilus 2A produce biosurfactant that is able to act as plant-growth promoting agent. Endophytic bacteria isolated from Chelidonium majus L. exhibit potential for hydrocarbons degradation and biosurfactant production. These properties provide promising perspectives for application of biosurfactants as potential agents for bioremediation of environment contaminated with hydrocarbons.