Matrix-Assisted DOSY for Analysis of Indole Alkaloid Mixtures
Diffusion-ordered spectroscopy (DOSY) is a powerful tool for investigating mixtures and identifying peaks of chemical components. However, similar diffusion coefficients of the components, particularly for complex mixtures that contain crowded resonances, limit resolution and restrict application of...
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doaj-0bd261052f9c4fc08ef84134066f6a312021-03-21T00:03:42ZengMDPI AGMolecules1420-30492021-03-01261751175110.3390/molecules26061751Matrix-Assisted DOSY for Analysis of Indole Alkaloid MixturesYu-Lin You0Fei-Fei Li1Ning Wang2Shu-Qi Wang3School of Pharmaceutical Sciences, Shandong University, 44# West Wenhua Road, Jinan 250012, ChinaSchool of Pharmaceutical Sciences, Shandong University, 44# West Wenhua Road, Jinan 250012, ChinaSchool of Pharmaceutical Sciences, Shandong University, 44# West Wenhua Road, Jinan 250012, ChinaSchool of Pharmaceutical Sciences, Shandong University, 44# West Wenhua Road, Jinan 250012, ChinaDiffusion-ordered spectroscopy (DOSY) is a powerful tool for investigating mixtures and identifying peaks of chemical components. However, similar diffusion coefficients of the components, particularly for complex mixtures that contain crowded resonances, limit resolution and restrict application of the DOSY technique. In this paper, matrix-assisted DOSY were used to explore whether the diffusion resolution of a complex model involving indole alkaloid mixtures can be realized. Furthermore, we investigated the influence of different factors on the separation effect. The results showed that the changes in diffusion coefficient differences were achieved more obviously when using sodium dodecyl sulfate (SDS) micelles as the matrix. In addition, we also found that increasing the concentration of SDS can improve the resolution of the DOSY spectrum. Finally, after investigating the influence factors and NMR conditions, we demonstrated the applications of the SDS-assisted DOSY on analyzing the total alkaloid extract of <i>Alstonia Mairei</i>, and the virtual separation of mixtures was achieved.https://www.mdpi.com/1420-3049/26/6/1751NMRDOSYsodium dodecyl sulfateindole alkaloid mixturesdiffusion resolution |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yu-Lin You Fei-Fei Li Ning Wang Shu-Qi Wang |
spellingShingle |
Yu-Lin You Fei-Fei Li Ning Wang Shu-Qi Wang Matrix-Assisted DOSY for Analysis of Indole Alkaloid Mixtures Molecules NMR DOSY sodium dodecyl sulfate indole alkaloid mixtures diffusion resolution |
author_facet |
Yu-Lin You Fei-Fei Li Ning Wang Shu-Qi Wang |
author_sort |
Yu-Lin You |
title |
Matrix-Assisted DOSY for Analysis of Indole Alkaloid Mixtures |
title_short |
Matrix-Assisted DOSY for Analysis of Indole Alkaloid Mixtures |
title_full |
Matrix-Assisted DOSY for Analysis of Indole Alkaloid Mixtures |
title_fullStr |
Matrix-Assisted DOSY for Analysis of Indole Alkaloid Mixtures |
title_full_unstemmed |
Matrix-Assisted DOSY for Analysis of Indole Alkaloid Mixtures |
title_sort |
matrix-assisted dosy for analysis of indole alkaloid mixtures |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2021-03-01 |
description |
Diffusion-ordered spectroscopy (DOSY) is a powerful tool for investigating mixtures and identifying peaks of chemical components. However, similar diffusion coefficients of the components, particularly for complex mixtures that contain crowded resonances, limit resolution and restrict application of the DOSY technique. In this paper, matrix-assisted DOSY were used to explore whether the diffusion resolution of a complex model involving indole alkaloid mixtures can be realized. Furthermore, we investigated the influence of different factors on the separation effect. The results showed that the changes in diffusion coefficient differences were achieved more obviously when using sodium dodecyl sulfate (SDS) micelles as the matrix. In addition, we also found that increasing the concentration of SDS can improve the resolution of the DOSY spectrum. Finally, after investigating the influence factors and NMR conditions, we demonstrated the applications of the SDS-assisted DOSY on analyzing the total alkaloid extract of <i>Alstonia Mairei</i>, and the virtual separation of mixtures was achieved. |
topic |
NMR DOSY sodium dodecyl sulfate indole alkaloid mixtures diffusion resolution |
url |
https://www.mdpi.com/1420-3049/26/6/1751 |
work_keys_str_mv |
AT yulinyou matrixassisteddosyforanalysisofindolealkaloidmixtures AT feifeili matrixassisteddosyforanalysisofindolealkaloidmixtures AT ningwang matrixassisteddosyforanalysisofindolealkaloidmixtures AT shuqiwang matrixassisteddosyforanalysisofindolealkaloidmixtures |
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1724211185109172224 |