Absolute counts of peripheral blood leukocyte subpopulations in intraabdominal sepsis and pneumonia-derived sepsis: a pilot study Absolute counts of peripheral blood leukocyte subpopulations in intraabdominal sepsis and pneumonia-derived sepsis: a pilot study

• Main Authors: Grazyna Anna Hoser, Tomasz Skirecki, Małgorzata Złotorowicz, Urszula Zielińska-Borkowska, Jerzy Kawiak
• Format: Article
• Language: English
• Published: Via Medica 2012-10-01
• Series: Folia Histochemica et Cytobiologica
• Online Access: http://czasopisma.viamedica.pl/fhc/article/view/19751
• ISSN: 0239-8508; 1897-5631

The leading pathophysiological changes during sepsis include systemic abnormalities in the immune&lt;br /&gt;response. Due to the general character of these disturbances, sepsis is usually studied as a homogenous clinical&lt;br /&gt;condition. We aimed to compare the immune response in intraabdominal sepsis (IAS) and pneumonia-derived&lt;br /&gt;sepsis (PDS). The following cell populations were examined: white blood cell count (WBC), monocytes, lymphocytes:&lt;br /&gt;CD3+, CD4+ and CD8+ T cells, B cells, and NK cells. In both studied groups (i.e. IAS and PDS), the&lt;br /&gt;WBC was elevated. However, it was significantly higher in the IAS group than in the PDS group. The difference&lt;br /&gt;was due to a lower granulocyte count, as well as a lower monocyte count in PDS. We found no significant&lt;br /&gt;correlation between the total lymphocyte number and CD3+CD8+ T cells in either form of sepsis. Similarly, we&lt;br /&gt;observed no correlation between the total lymphocyte number and the NK cells subset in IAS. However, the&lt;br /&gt;numbers of CD3+CD8+ and NK cells correlated similarly in both types of sepsis. Both studied types of sepsis&lt;br /&gt;induced profound lymphocytopenia, with marked loss of CD8+ T cells and the NK cells. However, the similar&lt;br /&gt;relation between them, which was independent of the infection type, suggests that the NK and CD3+CD8+ cells&lt;br /&gt;have shared mechanisms of regulation. The primary site of infection has an impact on the global immune reaction.&lt;br /&gt;These alternations include especially myeloid cells: granulocytes and monocytes which disappear from peripheral&lt;br /&gt;blood during PDS, but increase in IAS. &lt;br /&gt;<br>The leading pathophysiological changes during sepsis include systemic abnormalities in the immune&lt;br /&gt;response. Due to the general character of these disturbances, sepsis is usually studied as a homogenous clinical&lt;br /&gt;condition. We aimed to compare the immune response in intraabdominal sepsis (IAS) and pneumonia-derived&lt;br /&gt;sepsis (PDS). The following cell populations were examined: white blood cell count (WBC), monocytes, lymphocytes:&lt;br /&gt;CD3+, CD4+ and CD8+ T cells, B cells, and NK cells. In both studied groups (i.e. IAS and PDS), the&lt;br /&gt;WBC was elevated. However, it was significantly higher in the IAS group than in the PDS group. The difference&lt;br /&gt;was due to a lower granulocyte count, as well as a lower monocyte count in PDS. We found no significant&lt;br /&gt;correlation between the total lymphocyte number and CD3+CD8+ T cells in either form of sepsis. Similarly, we&lt;br /&gt;observed no correlation between the total lymphocyte number and the NK cells subset in IAS. However, the&lt;br /&gt;numbers of CD3+CD8+ and NK cells correlated similarly in both types of sepsis. Both studied types of sepsis&lt;br /&gt;induced profound lymphocytopenia, with marked loss of CD8+ T cells and the NK cells. However, the similar&lt;br /&gt;relation between them, which was independent of the infection type, suggests that the NK and CD3+CD8+ cells&lt;br /&gt;have shared mechanisms of regulation. The primary site of infection has an impact on the global immune reaction.&lt;br /&gt;These alternations include especially myeloid cells: granulocytes and monocytes which disappear from peripheral&lt;br /&gt;blood during PDS, but increase in IAS. &lt;br /&gt;