Enzymatic degradation study of PLA-based composite scaffolds
Disadvantages in the use of polylactic acid (PLA) as a base material for Tissue Engineering applications include the low osteoconductivity of this biomaterial, its acidic degradation and the deficient cellular adhesion on its surface. In order to counteract these drawbacks, calcium carbonate (CaCO3)...
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doaj-0bf4266996a74481b4f8f986f7047c9b2021-09-05T14:00:08ZengDe GruyterReviews on Advanced Materials Science1605-81272020-05-0159117017510.1515/rams-2020-0005rams-2020-0005Enzymatic degradation study of PLA-based composite scaffoldsDonate Ricardo0Monzón Mario1Alemán-Domínguez María Elena2Ortega Zaida3Departamento de Ingeniería Mecánica, Universidad de Las Palmas de Gran Canaria, Campus Universitario de Tafira s/n, 35017, Las Palmas, Spain; Tel.: +34-928-45-8603Departamento de Ingeniería Mecánica, Universidad de Las Palmas de Gran Canaria, Campus Universitario de Tafira s/n, 35017, Las Palmas, SpainDepartamento de Ingeniería Mecánica, Universidad de Las Palmas de Gran Canaria, Campus Universitario de Tafira s/n, 35017, Las Palmas, SpainDepartamento de Ingeniería de Procesos, Universidad de Las Palmas de Gran Canaria, Campus Universitario de Tafira s/n, 35017, Las Palmas, SpainDisadvantages in the use of polylactic acid (PLA) as a base material for Tissue Engineering applications include the low osteoconductivity of this biomaterial, its acidic degradation and the deficient cellular adhesion on its surface. In order to counteract these drawbacks, calcium carbonate (CaCO3) and β-tricalcium phosphate (Ca3(PO4)2, β-TCP) were proposed in this work as additives of PLA-based support structures. Composite scaffolds (PLA:CaCO3: β-TCP 95:2.5:2.5) manufactured by fused deposition modeling (FDM) were tested under enzymatic degradation using proteinase K enzymes to assess the modification of their properties in comparison with neat PLA scaffolds. The samples were characterized before and after the degradation test by optical microscopy, scanning electron microscopy, compression testing and thermogravimetric and calorimetric analysis. According to the results, the combination of the PLA matrix with the proposed additives increases the degradation rate of the 3D printed scaffolds, which is an advantage for the application of the composite scaffold in the field of Tissue Engineering. The higher degradation rate of the composite scaffolds could be explained by the release of the additive particles and the statistically higher microporosity of these samples compared to the neat PLA ones.https://doi.org/10.1515/rams-2020-0005polylactic acid (pla)bone tissue engineeringproteinase k |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Donate Ricardo Monzón Mario Alemán-Domínguez María Elena Ortega Zaida |
spellingShingle |
Donate Ricardo Monzón Mario Alemán-Domínguez María Elena Ortega Zaida Enzymatic degradation study of PLA-based composite scaffolds Reviews on Advanced Materials Science polylactic acid (pla) bone tissue engineering proteinase k |
author_facet |
Donate Ricardo Monzón Mario Alemán-Domínguez María Elena Ortega Zaida |
author_sort |
Donate Ricardo |
title |
Enzymatic degradation study of PLA-based composite scaffolds |
title_short |
Enzymatic degradation study of PLA-based composite scaffolds |
title_full |
Enzymatic degradation study of PLA-based composite scaffolds |
title_fullStr |
Enzymatic degradation study of PLA-based composite scaffolds |
title_full_unstemmed |
Enzymatic degradation study of PLA-based composite scaffolds |
title_sort |
enzymatic degradation study of pla-based composite scaffolds |
publisher |
De Gruyter |
series |
Reviews on Advanced Materials Science |
issn |
1605-8127 |
publishDate |
2020-05-01 |
description |
Disadvantages in the use of polylactic acid (PLA) as a base material for Tissue Engineering applications include the low osteoconductivity of this biomaterial, its acidic degradation and the deficient cellular adhesion on its surface. In order to counteract these drawbacks, calcium carbonate (CaCO3) and β-tricalcium phosphate (Ca3(PO4)2, β-TCP) were proposed in this work as additives of PLA-based support structures. Composite scaffolds (PLA:CaCO3: β-TCP 95:2.5:2.5) manufactured by fused deposition modeling (FDM) were tested under enzymatic degradation using proteinase K enzymes to assess the modification of their properties in comparison with neat PLA scaffolds. The samples were characterized before and after the degradation test by optical microscopy, scanning electron microscopy, compression testing and thermogravimetric and calorimetric analysis. According to the results, the combination of the PLA matrix with the proposed additives increases the degradation rate of the 3D printed scaffolds, which is an advantage for the application of the composite scaffold in the field of Tissue Engineering. The higher degradation rate of the composite scaffolds could be explained by the release of the additive particles and the statistically higher microporosity of these samples compared to the neat PLA ones. |
topic |
polylactic acid (pla) bone tissue engineering proteinase k |
url |
https://doi.org/10.1515/rams-2020-0005 |
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