Silver Nanoparticles in the Lung: Toxic Effects and Focal Accumulation of Silver in Remote Organs
The distribution of silver (Ag) into remote organs secondary to the application of Ag nanoparticles (Ag-NP) to the lung is still incompletely understood and was investigated in the rat with imaging methods. Dose-finding experiments were carried out with 50 nm- or 200 nm-sized polyvinyl pyrrolidine (...
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doaj-0bfbea3dfc9d48e49505b493751ffcec2020-11-24T22:04:12ZengMDPI AGNanomaterials2079-49912017-12-0171244110.3390/nano7120441nano7120441Silver Nanoparticles in the Lung: Toxic Effects and Focal Accumulation of Silver in Remote OrgansMartin Wiemann0Antje Vennemann1Franziska Blaske2Michael Sperling3Uwe Karst4IBE R&D Institute for Lung Health gGmbH, Mendelstr. 11, 48149 Münster, GermanyIBE R&D Institute for Lung Health gGmbH, Mendelstr. 11, 48149 Münster, GermanyInstitute of Inorganic and Analytical Chemistry, University of Münster, Corrensstr. 30, 48149 Münster, GermanyInstitute of Inorganic and Analytical Chemistry, University of Münster, Corrensstr. 30, 48149 Münster, GermanyInstitute of Inorganic and Analytical Chemistry, University of Münster, Corrensstr. 30, 48149 Münster, GermanyThe distribution of silver (Ag) into remote organs secondary to the application of Ag nanoparticles (Ag-NP) to the lung is still incompletely understood and was investigated in the rat with imaging methods. Dose-finding experiments were carried out with 50 nm- or 200 nm-sized polyvinyl pyrrolidine (PVP)-coated Ag-NP using alveolar macrophages in vitro and female rats, which received Ag-NP via intratracheal instillation. In the main study, we administered 37.5–300 µg per rat lung of the more toxic Ag50-PVP and assessed the broncho-alveolar lavage fluid (BALF) for inflammatory cells, total protein and fibronectin after three and 21 days. In parallel, lung tissue was analysed for DNA double-strand breaks and altered cell proliferation. While 75–150 µg Ag50-PVP per rat lung caused a reversible inflammation, 300 µg led to DNA damage, accelerated cell proliferation and progressively increasing numbers of neutrophilic granulocytes. Ag accumulation was significant in homogenates of liver and other peripheral organs upon lung dose of ≥75 µg. Quantitative laser-ablation inductively-coupled plasma mass spectrometry (LA-ICP-MS) combined with enhanced dark field microscopy and autometallography revealed focal accumulations of Ag and/or Ag-NP in sections of peripheral organs: mediastinal lymph nodes contained Ag-NP especially in peripheral macrophages and Ag in argyrophilic fibres. In the kidney, Ag had accumulated within proximal tubuli, while renal filter structures contained no Ag. Discrete localizations were also observed in immune cells of liver and spleen. Overall, the study shows that concentrations of Ag-NP, which elicit a transient inflammation in the rat lung, lead to focal accumulations of Ag in peripheral organs, and this might pose a risk to particular cell populations in remote sites.https://www.mdpi.com/2079-4991/7/12/441silver nanoparticlequantitative bio-imagingin vitro toxicitynanoparticle transition |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Martin Wiemann Antje Vennemann Franziska Blaske Michael Sperling Uwe Karst |
spellingShingle |
Martin Wiemann Antje Vennemann Franziska Blaske Michael Sperling Uwe Karst Silver Nanoparticles in the Lung: Toxic Effects and Focal Accumulation of Silver in Remote Organs Nanomaterials silver nanoparticle quantitative bio-imaging in vitro toxicity nanoparticle transition |
author_facet |
Martin Wiemann Antje Vennemann Franziska Blaske Michael Sperling Uwe Karst |
author_sort |
Martin Wiemann |
title |
Silver Nanoparticles in the Lung: Toxic Effects and Focal Accumulation of Silver in Remote Organs |
title_short |
Silver Nanoparticles in the Lung: Toxic Effects and Focal Accumulation of Silver in Remote Organs |
title_full |
Silver Nanoparticles in the Lung: Toxic Effects and Focal Accumulation of Silver in Remote Organs |
title_fullStr |
Silver Nanoparticles in the Lung: Toxic Effects and Focal Accumulation of Silver in Remote Organs |
title_full_unstemmed |
Silver Nanoparticles in the Lung: Toxic Effects and Focal Accumulation of Silver in Remote Organs |
title_sort |
silver nanoparticles in the lung: toxic effects and focal accumulation of silver in remote organs |
publisher |
MDPI AG |
series |
Nanomaterials |
issn |
2079-4991 |
publishDate |
2017-12-01 |
description |
The distribution of silver (Ag) into remote organs secondary to the application of Ag nanoparticles (Ag-NP) to the lung is still incompletely understood and was investigated in the rat with imaging methods. Dose-finding experiments were carried out with 50 nm- or 200 nm-sized polyvinyl pyrrolidine (PVP)-coated Ag-NP using alveolar macrophages in vitro and female rats, which received Ag-NP via intratracheal instillation. In the main study, we administered 37.5–300 µg per rat lung of the more toxic Ag50-PVP and assessed the broncho-alveolar lavage fluid (BALF) for inflammatory cells, total protein and fibronectin after three and 21 days. In parallel, lung tissue was analysed for DNA double-strand breaks and altered cell proliferation. While 75–150 µg Ag50-PVP per rat lung caused a reversible inflammation, 300 µg led to DNA damage, accelerated cell proliferation and progressively increasing numbers of neutrophilic granulocytes. Ag accumulation was significant in homogenates of liver and other peripheral organs upon lung dose of ≥75 µg. Quantitative laser-ablation inductively-coupled plasma mass spectrometry (LA-ICP-MS) combined with enhanced dark field microscopy and autometallography revealed focal accumulations of Ag and/or Ag-NP in sections of peripheral organs: mediastinal lymph nodes contained Ag-NP especially in peripheral macrophages and Ag in argyrophilic fibres. In the kidney, Ag had accumulated within proximal tubuli, while renal filter structures contained no Ag. Discrete localizations were also observed in immune cells of liver and spleen. Overall, the study shows that concentrations of Ag-NP, which elicit a transient inflammation in the rat lung, lead to focal accumulations of Ag in peripheral organs, and this might pose a risk to particular cell populations in remote sites. |
topic |
silver nanoparticle quantitative bio-imaging in vitro toxicity nanoparticle transition |
url |
https://www.mdpi.com/2079-4991/7/12/441 |
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