Plasmatic exosomes from prostate cancer patients show increased carbonic anhydrase IX expression and activity and low pH
Acidity, hypoxia and increased release of exosomes are severe phenotypes of tumours. The regulation of pH in tumours involves the interaction of several proteins, including the carbonic anhydrases which catalyze the formation of bicarbonate and protons from carbon dioxide and water. Among CA isoform...
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doaj-0c0471ad122741b4ad967ed6f88a9d6b2021-07-15T13:10:31ZengTaylor & Francis GroupJournal of Enzyme Inhibition and Medicinal Chemistry1475-63661475-63742020-01-0135128028810.1080/14756366.2019.16972491697249Plasmatic exosomes from prostate cancer patients show increased carbonic anhydrase IX expression and activity and low pHMariantonia Logozzi0Davide Mizzoni1Clemente Capasso2Sonia Del Prete3Rossella Di Raimo4Mario Falchi5Daniela F. Angelini6Alessandro Sciarra7Martina Maggi8Claudiu T. Supuran9Stefano Fais10Department of Oncology and Molecular Medicine, Istituto Superiore di SanitàDepartment of Oncology and Molecular Medicine, Istituto Superiore di SanitàNational Research Council, Institute of Biosciences and BioResourcesNational Research Council, Institute of Biosciences and BioResourcesDepartment of Oncology and Molecular Medicine, Istituto Superiore di SanitàNational AIDS Center, Istituto Superiore di SanitàNeuroimmunology Unit, IRCCS Santa Lucia FoundationDepartment of Urology, Policlinico Umberto I, Sapienza University of RomeDepartment of Urology, Policlinico Umberto I, Sapienza University of RomeNEUROFARBA Department, University of Florence, Section of Pharmaceutical ChemistryDepartment of Oncology and Molecular Medicine, Istituto Superiore di SanitàAcidity, hypoxia and increased release of exosomes are severe phenotypes of tumours. The regulation of pH in tumours involves the interaction of several proteins, including the carbonic anhydrases which catalyze the formation of bicarbonate and protons from carbon dioxide and water. Among CA isoforms, CA IX is over-expressed in a large number of solid tumours, conferring to cancer cells a survival advantage in hypoxic and acidic microenvironment, but there isn’t evidence that CA IX expression could have a real clinical impact. Therefore, in this study for the first time the expression and activity of CA IX have been investigated in the plasmatic exosomes obtained from patients with prostate carcinoma (PCa). For this purpose, the study was performed through different methodological approaches, such as NTA, western blot analysis, enzyme activity assay, Nanoscale flow cytometry, ELISA, confocal microscopy. The results showed that PCa exosomes significantly overexpressed CA IX levels and related activity as compared to healthy donors. Furthermore, CA IX expression and activity were correlated to the exosome intraluminal pH, demonstrating for the first time that PCa exosomes are acidic. Our data suggest the possible use of the exosomal CA IX expression and activity as a biomarker of cancer progression in PCa.http://dx.doi.org/10.1080/14756366.2019.1697249acidic tumour microenvironmentprostate cancercarbonic anhydrase ixplasmatic exosomesbiomarker |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mariantonia Logozzi Davide Mizzoni Clemente Capasso Sonia Del Prete Rossella Di Raimo Mario Falchi Daniela F. Angelini Alessandro Sciarra Martina Maggi Claudiu T. Supuran Stefano Fais |
spellingShingle |
Mariantonia Logozzi Davide Mizzoni Clemente Capasso Sonia Del Prete Rossella Di Raimo Mario Falchi Daniela F. Angelini Alessandro Sciarra Martina Maggi Claudiu T. Supuran Stefano Fais Plasmatic exosomes from prostate cancer patients show increased carbonic anhydrase IX expression and activity and low pH Journal of Enzyme Inhibition and Medicinal Chemistry acidic tumour microenvironment prostate cancer carbonic anhydrase ix plasmatic exosomes biomarker |
author_facet |
Mariantonia Logozzi Davide Mizzoni Clemente Capasso Sonia Del Prete Rossella Di Raimo Mario Falchi Daniela F. Angelini Alessandro Sciarra Martina Maggi Claudiu T. Supuran Stefano Fais |
author_sort |
Mariantonia Logozzi |
title |
Plasmatic exosomes from prostate cancer patients show increased carbonic anhydrase IX expression and activity and low pH |
title_short |
Plasmatic exosomes from prostate cancer patients show increased carbonic anhydrase IX expression and activity and low pH |
title_full |
Plasmatic exosomes from prostate cancer patients show increased carbonic anhydrase IX expression and activity and low pH |
title_fullStr |
Plasmatic exosomes from prostate cancer patients show increased carbonic anhydrase IX expression and activity and low pH |
title_full_unstemmed |
Plasmatic exosomes from prostate cancer patients show increased carbonic anhydrase IX expression and activity and low pH |
title_sort |
plasmatic exosomes from prostate cancer patients show increased carbonic anhydrase ix expression and activity and low ph |
publisher |
Taylor & Francis Group |
series |
Journal of Enzyme Inhibition and Medicinal Chemistry |
issn |
1475-6366 1475-6374 |
publishDate |
2020-01-01 |
description |
Acidity, hypoxia and increased release of exosomes are severe phenotypes of tumours. The regulation of pH in tumours involves the interaction of several proteins, including the carbonic anhydrases which catalyze the formation of bicarbonate and protons from carbon dioxide and water. Among CA isoforms, CA IX is over-expressed in a large number of solid tumours, conferring to cancer cells a survival advantage in hypoxic and acidic microenvironment, but there isn’t evidence that CA IX expression could have a real clinical impact. Therefore, in this study for the first time the expression and activity of CA IX have been investigated in the plasmatic exosomes obtained from patients with prostate carcinoma (PCa). For this purpose, the study was performed through different methodological approaches, such as NTA, western blot analysis, enzyme activity assay, Nanoscale flow cytometry, ELISA, confocal microscopy. The results showed that PCa exosomes significantly overexpressed CA IX levels and related activity as compared to healthy donors. Furthermore, CA IX expression and activity were correlated to the exosome intraluminal pH, demonstrating for the first time that PCa exosomes are acidic. Our data suggest the possible use of the exosomal CA IX expression and activity as a biomarker of cancer progression in PCa. |
topic |
acidic tumour microenvironment prostate cancer carbonic anhydrase ix plasmatic exosomes biomarker |
url |
http://dx.doi.org/10.1080/14756366.2019.1697249 |
work_keys_str_mv |
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