Direct determination of E and Z configurations for double bond in bioactive meroterpenoids from Ganoderma mushrooms by diagnostic 1H NMR chemical shifts and structure revisions of previous analogues
Ganoderma mushrooms are not only traditional Chinese medicine, but also dietary supplements and functional foods worldwide. In this work, six new meroterpenoids, ganodercins A − F (2, 3, and 6–9), and three known compounds ganomycin C (1), cochlearin I (4), and fornicin D (5) were isolated from Gano...
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doaj-0c0f7b2ee49040d6808bbfed47420c502021-09-21T04:08:57ZengElsevierJournal of Functional Foods1756-46462021-12-0187104758Direct determination of E and Z configurations for double bond in bioactive meroterpenoids from Ganoderma mushrooms by diagnostic 1H NMR chemical shifts and structure revisions of previous analoguesFu-Ying Qin0Jiao-Jiao Zhang1Dai-Wei Wang2Te Xu3Dan Cai4Yong-Xian Cheng5Institute for Inheritance-Based Innovation of Chinese Medicine, School of Pharmaceutical Sciences, Health Science Center, Shenzhen University, Shenzhen 518060, People’s Republic of China; Institute of Microscale Optoelectronics, Shenzhen University, Shenzhen 518060, People’s Republic of ChinaInstitute for Inheritance-Based Innovation of Chinese Medicine, School of Pharmaceutical Sciences, Health Science Center, Shenzhen University, Shenzhen 518060, People’s Republic of China; Institute of Microscale Optoelectronics, Shenzhen University, Shenzhen 518060, People’s Republic of ChinaInstitute for Inheritance-Based Innovation of Chinese Medicine, School of Pharmaceutical Sciences, Health Science Center, Shenzhen University, Shenzhen 518060, People’s Republic of ChinaInstitute for Inheritance-Based Innovation of Chinese Medicine, School of Pharmaceutical Sciences, Health Science Center, Shenzhen University, Shenzhen 518060, People’s Republic of ChinaInstitute for Inheritance-Based Innovation of Chinese Medicine, School of Pharmaceutical Sciences, Health Science Center, Shenzhen University, Shenzhen 518060, People’s Republic of ChinaInstitute for Inheritance-Based Innovation of Chinese Medicine, School of Pharmaceutical Sciences, Health Science Center, Shenzhen University, Shenzhen 518060, People’s Republic of China; Guangdong Key Laboratory for Functional Substances in Medicinal Edible Resources and Healthcare Products, School of Life Sciences and Food Engineering, Hanshan Normal University, Chaozhou 521041, People’s Republic of China; Corresponding author at: Institute for Inheritance-Based Innovation of Chinese Medicine, School of Pharmaceutical Sciences, Health Science Center, Shenzhen University, Shenzhen 518060, People’s Republic of China.Ganoderma mushrooms are not only traditional Chinese medicine, but also dietary supplements and functional foods worldwide. In this work, six new meroterpenoids, ganodercins A − F (2, 3, and 6–9), and three known compounds ganomycin C (1), cochlearin I (4), and fornicin D (5) were isolated from Ganoderma mushrooms. An approach characteristic of the chemical shift for H-8 to assign the geometry of meroterpenoids was developed and successfully used for structure revisions of 1, 5, and previous analogues. Biological evaluation found that 4 selectively inhibits Smad3 phosphorylation in TGF-β1-induced NRK-52E cells. Moreover, 1 and 7 could increase glucose uptake in a dose dependent manner in palmitic acid induced C2C12 cells. Further, 1 was found to activate AMPKα(Thr-172) and AKT(Ser-473) phosphorylation, while 7 could activate AKT(Ser-473) phosphorylation. This study demonstrates the potential of Ganoderma to be developed as new anti-renal fibrosis and improve insulin resistance functional food and medicine.http://www.sciencedirect.com/science/article/pii/S1756464621004072Ganoderma lucidumGanoderma cochlearMeroterpenoidsInsulin resistanceRenal fibrosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fu-Ying Qin Jiao-Jiao Zhang Dai-Wei Wang Te Xu Dan Cai Yong-Xian Cheng |
spellingShingle |
Fu-Ying Qin Jiao-Jiao Zhang Dai-Wei Wang Te Xu Dan Cai Yong-Xian Cheng Direct determination of E and Z configurations for double bond in bioactive meroterpenoids from Ganoderma mushrooms by diagnostic 1H NMR chemical shifts and structure revisions of previous analogues Journal of Functional Foods Ganoderma lucidum Ganoderma cochlear Meroterpenoids Insulin resistance Renal fibrosis |
author_facet |
Fu-Ying Qin Jiao-Jiao Zhang Dai-Wei Wang Te Xu Dan Cai Yong-Xian Cheng |
author_sort |
Fu-Ying Qin |
title |
Direct determination of E and Z configurations for double bond in bioactive meroterpenoids from Ganoderma mushrooms by diagnostic 1H NMR chemical shifts and structure revisions of previous analogues |
title_short |
Direct determination of E and Z configurations for double bond in bioactive meroterpenoids from Ganoderma mushrooms by diagnostic 1H NMR chemical shifts and structure revisions of previous analogues |
title_full |
Direct determination of E and Z configurations for double bond in bioactive meroterpenoids from Ganoderma mushrooms by diagnostic 1H NMR chemical shifts and structure revisions of previous analogues |
title_fullStr |
Direct determination of E and Z configurations for double bond in bioactive meroterpenoids from Ganoderma mushrooms by diagnostic 1H NMR chemical shifts and structure revisions of previous analogues |
title_full_unstemmed |
Direct determination of E and Z configurations for double bond in bioactive meroterpenoids from Ganoderma mushrooms by diagnostic 1H NMR chemical shifts and structure revisions of previous analogues |
title_sort |
direct determination of e and z configurations for double bond in bioactive meroterpenoids from ganoderma mushrooms by diagnostic 1h nmr chemical shifts and structure revisions of previous analogues |
publisher |
Elsevier |
series |
Journal of Functional Foods |
issn |
1756-4646 |
publishDate |
2021-12-01 |
description |
Ganoderma mushrooms are not only traditional Chinese medicine, but also dietary supplements and functional foods worldwide. In this work, six new meroterpenoids, ganodercins A − F (2, 3, and 6–9), and three known compounds ganomycin C (1), cochlearin I (4), and fornicin D (5) were isolated from Ganoderma mushrooms. An approach characteristic of the chemical shift for H-8 to assign the geometry of meroterpenoids was developed and successfully used for structure revisions of 1, 5, and previous analogues. Biological evaluation found that 4 selectively inhibits Smad3 phosphorylation in TGF-β1-induced NRK-52E cells. Moreover, 1 and 7 could increase glucose uptake in a dose dependent manner in palmitic acid induced C2C12 cells. Further, 1 was found to activate AMPKα(Thr-172) and AKT(Ser-473) phosphorylation, while 7 could activate AKT(Ser-473) phosphorylation. This study demonstrates the potential of Ganoderma to be developed as new anti-renal fibrosis and improve insulin resistance functional food and medicine. |
topic |
Ganoderma lucidum Ganoderma cochlear Meroterpenoids Insulin resistance Renal fibrosis |
url |
http://www.sciencedirect.com/science/article/pii/S1756464621004072 |
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