Structural engineering of graphene for high‐resolution cryo‐electron microscopy
Abstract The revolutionary improvement of hardware and algorithm in cryogenic electron microscopy (cryo‐EM) has made it a routine method to obtain structures of macromolecules at near‐atomic resolution. Nevertheless, this technique still faces many challenges. The structure‐solving efficiency of cry...
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Online Access: | https://doi.org/10.1002/smm2.1045 |
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doaj-0c155c030cb04a72bc7942fdadbd9d5e2021-06-30T02:41:14ZengWileySmartMat2688-819X2021-07-012220221210.1002/smm2.1045Structural engineering of graphene for high‐resolution cryo‐electron microscopyJie Xu0Xiaoya Cui1Nan Liu2Yanan Chen3Hong‐Wei Wang4Ministry of Education Key Laboratory of Protein Sciences, Beijing Advanced Innovation Center for Structural Biology, School of Life Sciences Tsinghua University Beijing ChinaMinistry of Education Key Laboratory of Protein Sciences, Beijing Advanced Innovation Center for Structural Biology, School of Life Sciences Tsinghua University Beijing ChinaMinistry of Education Key Laboratory of Protein Sciences, Beijing Advanced Innovation Center for Structural Biology, School of Life Sciences Tsinghua University Beijing ChinaMinistry of Education Key Laboratory of Protein Sciences, Beijing Advanced Innovation Center for Structural Biology, School of Life Sciences Tsinghua University Beijing ChinaMinistry of Education Key Laboratory of Protein Sciences, Beijing Advanced Innovation Center for Structural Biology, School of Life Sciences Tsinghua University Beijing ChinaAbstract The revolutionary improvement of hardware and algorithm in cryogenic electron microscopy (cryo‐EM) has made it a routine method to obtain structures of macromolecules at near‐atomic resolution. Nevertheless, this technique still faces many challenges. The structure‐solving efficiency of cryo‐EM can be significantly reduced by the biomolecules' denaturation on the air–water interfaces, the preferred orientation, strong background noise from supporting films and particle motion, and so forth. To overcome these problems, nanomaterials with ultrahigh electronic conductivity and ultrathin thickness are explored as promising cryo‐EM specimen supporting films. Herein, we summarize the structural engineering of graphene, for example, surface and interface modification, as supporting films for grids and the application on high‐resolution cryo‐EM and discuss potential future perspectives.https://doi.org/10.1002/smm2.1045graphenehigh‐resolution cryogenic electron microscopylife scienceliquid celltomography |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jie Xu Xiaoya Cui Nan Liu Yanan Chen Hong‐Wei Wang |
spellingShingle |
Jie Xu Xiaoya Cui Nan Liu Yanan Chen Hong‐Wei Wang Structural engineering of graphene for high‐resolution cryo‐electron microscopy SmartMat graphene high‐resolution cryogenic electron microscopy life science liquid cell tomography |
author_facet |
Jie Xu Xiaoya Cui Nan Liu Yanan Chen Hong‐Wei Wang |
author_sort |
Jie Xu |
title |
Structural engineering of graphene for high‐resolution cryo‐electron microscopy |
title_short |
Structural engineering of graphene for high‐resolution cryo‐electron microscopy |
title_full |
Structural engineering of graphene for high‐resolution cryo‐electron microscopy |
title_fullStr |
Structural engineering of graphene for high‐resolution cryo‐electron microscopy |
title_full_unstemmed |
Structural engineering of graphene for high‐resolution cryo‐electron microscopy |
title_sort |
structural engineering of graphene for high‐resolution cryo‐electron microscopy |
publisher |
Wiley |
series |
SmartMat |
issn |
2688-819X |
publishDate |
2021-07-01 |
description |
Abstract The revolutionary improvement of hardware and algorithm in cryogenic electron microscopy (cryo‐EM) has made it a routine method to obtain structures of macromolecules at near‐atomic resolution. Nevertheless, this technique still faces many challenges. The structure‐solving efficiency of cryo‐EM can be significantly reduced by the biomolecules' denaturation on the air–water interfaces, the preferred orientation, strong background noise from supporting films and particle motion, and so forth. To overcome these problems, nanomaterials with ultrahigh electronic conductivity and ultrathin thickness are explored as promising cryo‐EM specimen supporting films. Herein, we summarize the structural engineering of graphene, for example, surface and interface modification, as supporting films for grids and the application on high‐resolution cryo‐EM and discuss potential future perspectives. |
topic |
graphene high‐resolution cryogenic electron microscopy life science liquid cell tomography |
url |
https://doi.org/10.1002/smm2.1045 |
work_keys_str_mv |
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1721353571449962496 |