Proteomic profiling of liver from Elaphe taeniura, a common snake in eastern and southeastern Asia
Snake liver has been implicated in the adaptation of snakes to a variety of habitats. However, to date, there has been no systematic analysis of snake liver proteins. In this study, we undertook a proteomic analysis of liver from the colubrid snake Elaphe taeniura using a combination of two-dimensio...
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2013-01-01
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doaj-0c1c3d1a2a224a7ca9f05cef52b537fb2020-11-25T00:12:31ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1678-46852013-01-0136343844710.1590/S1415-47572013000300020S1415-47572013000300020Proteomic profiling of liver from Elaphe taeniura, a common snake in eastern and southeastern AsiaLiang Chen0Hengchuan Xia1Yiting Wang2Keping Chen3Lvgao Qin4Bin Wang5Qin Yao6Jun Li7Yuanqing He8Ermi Zhao9Sichuan UniversityJiangsu UniversityYangzhou UniversityJiangsu UniversityJiangsu UniversityJiangsu UniversityJiangsu UniversityJiangsu UniversityJiangsu UniversitySichuan UniversitySnake liver has been implicated in the adaptation of snakes to a variety of habitats. However, to date, there has been no systematic analysis of snake liver proteins. In this study, we undertook a proteomic analysis of liver from the colubrid snake Elaphe taeniura using a combination of two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time of flightmass spectrometry (MALDI-TOF MS). We also constructed a local protein sequence database based on transcriptome sequencing to facilitate protein identification. Of the 268 protein spots revealed by 2-DE 109 gave positive MS signals, 84 of which were identified by searching the NCBInr, Swiss-Prot and local databases. The other 25 protein spots could not be identified, possibly because their transcripts were not be stable enough to be detected by transcriptome sequencing. GO analysis showed that most proteins may be involved in binding, catalysis, cellular processes and metabolic processes. Forty-two of the liver proteins identified were found in other reptiles and in amphibians. The findings of this study provide a good reference map of snake liver proteins that will be useful in molecular investigations of snake physiology and adaptation.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300020&lng=en&tlng=en2D electrophoresisElaphe taeniuraMALDI-TOF mass spectrometryprotein |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Liang Chen Hengchuan Xia Yiting Wang Keping Chen Lvgao Qin Bin Wang Qin Yao Jun Li Yuanqing He Ermi Zhao |
spellingShingle |
Liang Chen Hengchuan Xia Yiting Wang Keping Chen Lvgao Qin Bin Wang Qin Yao Jun Li Yuanqing He Ermi Zhao Proteomic profiling of liver from Elaphe taeniura, a common snake in eastern and southeastern Asia Genetics and Molecular Biology 2D electrophoresis Elaphe taeniura MALDI-TOF mass spectrometry protein |
author_facet |
Liang Chen Hengchuan Xia Yiting Wang Keping Chen Lvgao Qin Bin Wang Qin Yao Jun Li Yuanqing He Ermi Zhao |
author_sort |
Liang Chen |
title |
Proteomic profiling of liver from Elaphe taeniura, a common snake in eastern and southeastern Asia |
title_short |
Proteomic profiling of liver from Elaphe taeniura, a common snake in eastern and southeastern Asia |
title_full |
Proteomic profiling of liver from Elaphe taeniura, a common snake in eastern and southeastern Asia |
title_fullStr |
Proteomic profiling of liver from Elaphe taeniura, a common snake in eastern and southeastern Asia |
title_full_unstemmed |
Proteomic profiling of liver from Elaphe taeniura, a common snake in eastern and southeastern Asia |
title_sort |
proteomic profiling of liver from elaphe taeniura, a common snake in eastern and southeastern asia |
publisher |
Sociedade Brasileira de Genética |
series |
Genetics and Molecular Biology |
issn |
1678-4685 |
publishDate |
2013-01-01 |
description |
Snake liver has been implicated in the adaptation of snakes to a variety of habitats. However, to date, there has been no systematic analysis of snake liver proteins. In this study, we undertook a proteomic analysis of liver from the colubrid snake Elaphe taeniura using a combination of two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time of flightmass spectrometry (MALDI-TOF MS). We also constructed a local protein sequence database based on transcriptome sequencing to facilitate protein identification. Of the 268 protein spots revealed by 2-DE 109 gave positive MS signals, 84 of which were identified by searching the NCBInr, Swiss-Prot and local databases. The other 25 protein spots could not be identified, possibly because their transcripts were not be stable enough to be detected by transcriptome sequencing. GO analysis showed that most proteins may be involved in binding, catalysis, cellular processes and metabolic processes. Forty-two of the liver proteins identified were found in other reptiles and in amphibians. The findings of this study provide a good reference map of snake liver proteins that will be useful in molecular investigations of snake physiology and adaptation. |
topic |
2D electrophoresis Elaphe taeniura MALDI-TOF mass spectrometry protein |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300020&lng=en&tlng=en |
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