Protective effects elicited by cow milk fermented with L. Paracasei CBAL74 against SARS-CoV-2 infection in human enterocytes

Protective effects elicited by cow milk fermented with L. Paracasei CBAL74 against SARS-CoV-2 infection in human enterocytes

Fermented foods have been proposed in limiting SARS-CoV-2 infection. Emerging evidence suggest the efficacy of cow’s milk fermented with the probiotic L. paracasei CBAL74 (FM-CBAL74) in preventing infectious diseases. We evaluated the protective action of FM-CBAL74 against SARS-CoV-2 infection in hu...

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Main Authors: Lorella Paparo, Cristina Bruno, Veronica Ferrucci, Erika Punto, Maurizio Viscardi, Giovanna Fusco, Pellegrino Cerino, Alessia Romano, Massimo Zollo, Roberto Berni Canani
Format: Article
Language:English
Published: Elsevier 2021-12-01
Series:Journal of Functional Foods
Subjects:
COVID-19
Fermented foods
Viral infection
Angiotensin-converting enzyme-2
Transmembrane protease serine 2
Intestinal infection
Online Access:http://www.sciencedirect.com/science/article/pii/S1756464621004369
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spelling doaj-0c205d09532a4a6b85b2081ce76a4ca92021-10-07T04:24:24ZengElsevierJournal of Functional Foods1756-46462021-12-0187104787Protective effects elicited by cow milk fermented with L. Paracasei CBAL74 against SARS-CoV-2 infection in human enterocytesLorella Paparo0Cristina Bruno1Veronica Ferrucci2Erika Punto3Maurizio Viscardi4Giovanna Fusco5Pellegrino Cerino6Alessia Romano7Massimo Zollo8Roberto Berni Canani9Department of Translational Medical Science, University of Naples, “Federico II”, Naples, Italy; CEINGE – Advanced Biotechnologies Research Center s.c.ar.l., University of Naples “Federico II”, Naples, ItalyDepartment of Translational Medical Science, University of Naples, “Federico II”, Naples, Italy; CEINGE – Advanced Biotechnologies Research Center s.c.ar.l., University of Naples “Federico II”, Naples, ItalyCEINGE – Advanced Biotechnologies Research Center s.c.ar.l., University of Naples “Federico II”, Naples, Italy; Dipartimento di Medicina Molecolare e Biotecnologie Mediche (DMMBM), University of Naples “Federico II”, Naples, ItalyDepartment of Translational Medical Science, University of Naples, “Federico II”, Naples, Italy; CEINGE – Advanced Biotechnologies Research Center s.c.ar.l., University of Naples “Federico II”, Naples, ItalyDAI Medicina di Laboratorio e Trasfusionale, AOU Azienda Ospedaliera, University of Naples “Federico II”, Naples, ItalyDAI Medicina di Laboratorio e Trasfusionale, AOU Azienda Ospedaliera, University of Naples “Federico II”, Naples, ItalyDAI Medicina di Laboratorio e Trasfusionale, AOU Azienda Ospedaliera, University of Naples “Federico II”, Naples, ItalyCEINGE – Advanced Biotechnologies Research Center s.c.ar.l., University of Naples “Federico II”, Naples, ItalyCEINGE – Advanced Biotechnologies Research Center s.c.ar.l., University of Naples “Federico II”, Naples, Italy; Dipartimento di Medicina Molecolare e Biotecnologie Mediche (DMMBM), University of Naples “Federico II”, Naples, ItalyDepartment of Translational Medical Science, University of Naples, “Federico II”, Naples, Italy; CEINGE – Advanced Biotechnologies Research Center s.c.ar.l., University of Naples “Federico II”, Naples, Italy; European Laboratory for the Investigation of Food-Induced Diseases, University of Naples “Federico II”, Naples, Italy; Task Force for Microbiome Studies, University of Naples “Federico II”, Naples, Italy; Task Force for Nutraceuticals and Functional Foods, University of Naples “Federico II”, Naples, Italy; Corresponding author at: Chief of the Pediatric Allergy Program at the Department of Translational Medical Science, Chief of ImmunoNutritionLab at CEINGE – Advanced Biotechnologies Research Center, University of Naples, “Federico II”, Via S. Pansini, 5, 80131 Naples, Italy.Fermented foods have been proposed in limiting SARS-CoV-2 infection. Emerging evidence suggest the efficacy of cow’s milk fermented with the probiotic L. paracasei CBAL74 (FM-CBAL74) in preventing infectious diseases. We evaluated the protective action of FM-CBAL74 against SARS-CoV-2 infection in human enterocytes. Relevant aspects of SARS-CoV-2 infection were assessed: infectivity, host functional receptor angiotensin-converting enzyme-2 (ACE2), transmembrane protease serine 2 (TMPRSS2), and pro-inflammatory cytokines expression (IL-6, IL-15, IL-1β, VEGFβ, TNF-α, MCP-1, CXCL1).Pre-incubation with FM-CBA L74 reduced the number of infected cells. The expression of ACE2 and the pro-inflammatory cytokines IL-6, VEGFβ, IL-15, IL-1β was downregulated by the pre-treatment with this fermented food. No effect on TMPRSS2, MCP-1, TNF-α and CXCL1 expression was observed.Modulating the crucial aspects of the infection, the fermented food FM-CBAL74 exerts a preventive action against SARS-CoV-2. These evidence could pave the way to innovative nutritional strategy to mitigate the COVID-19.http://www.sciencedirect.com/science/article/pii/S1756464621004369COVID-19Fermented foodsViral infectionAngiotensin-converting enzyme-2Transmembrane protease serine 2Intestinal infection
collection DOAJ
language English
format Article
sources DOAJ
author Lorella Paparo
Cristina Bruno
Veronica Ferrucci
Erika Punto
Maurizio Viscardi
Giovanna Fusco
Pellegrino Cerino
Alessia Romano
Massimo Zollo
Roberto Berni Canani
spellingShingle Lorella Paparo
Cristina Bruno
Veronica Ferrucci
Erika Punto
Maurizio Viscardi
Giovanna Fusco
Pellegrino Cerino
Alessia Romano
Massimo Zollo
Roberto Berni Canani
Protective effects elicited by cow milk fermented with L. Paracasei CBAL74 against SARS-CoV-2 infection in human enterocytes
Journal of Functional Foods
COVID-19
Fermented foods
Viral infection
Angiotensin-converting enzyme-2
Transmembrane protease serine 2
Intestinal infection
author_facet Lorella Paparo
Cristina Bruno
Veronica Ferrucci
Erika Punto
Maurizio Viscardi
Giovanna Fusco
Pellegrino Cerino
Alessia Romano
Massimo Zollo
Roberto Berni Canani
author_sort Lorella Paparo
title Protective effects elicited by cow milk fermented with L. Paracasei CBAL74 against SARS-CoV-2 infection in human enterocytes
title_short Protective effects elicited by cow milk fermented with L. Paracasei CBAL74 against SARS-CoV-2 infection in human enterocytes
title_full Protective effects elicited by cow milk fermented with L. Paracasei CBAL74 against SARS-CoV-2 infection in human enterocytes
title_fullStr Protective effects elicited by cow milk fermented with L. Paracasei CBAL74 against SARS-CoV-2 infection in human enterocytes
title_full_unstemmed Protective effects elicited by cow milk fermented with L. Paracasei CBAL74 against SARS-CoV-2 infection in human enterocytes
title_sort protective effects elicited by cow milk fermented with l. paracasei cbal74 against sars-cov-2 infection in human enterocytes
publisher Elsevier
series Journal of Functional Foods
issn 1756-4646
publishDate 2021-12-01
description Fermented foods have been proposed in limiting SARS-CoV-2 infection. Emerging evidence suggest the efficacy of cow’s milk fermented with the probiotic L. paracasei CBAL74 (FM-CBAL74) in preventing infectious diseases. We evaluated the protective action of FM-CBAL74 against SARS-CoV-2 infection in human enterocytes. Relevant aspects of SARS-CoV-2 infection were assessed: infectivity, host functional receptor angiotensin-converting enzyme-2 (ACE2), transmembrane protease serine 2 (TMPRSS2), and pro-inflammatory cytokines expression (IL-6, IL-15, IL-1β, VEGFβ, TNF-α, MCP-1, CXCL1).Pre-incubation with FM-CBA L74 reduced the number of infected cells. The expression of ACE2 and the pro-inflammatory cytokines IL-6, VEGFβ, IL-15, IL-1β was downregulated by the pre-treatment with this fermented food. No effect on TMPRSS2, MCP-1, TNF-α and CXCL1 expression was observed.Modulating the crucial aspects of the infection, the fermented food FM-CBAL74 exerts a preventive action against SARS-CoV-2. These evidence could pave the way to innovative nutritional strategy to mitigate the COVID-19.
topic COVID-19
Fermented foods
Viral infection
Angiotensin-converting enzyme-2
Transmembrane protease serine 2
Intestinal infection
url http://www.sciencedirect.com/science/article/pii/S1756464621004369
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