<i>Orthosiphon stamineus</i> Proteins Alleviate Pentylenetetrazol-Induced Seizures in Zebrafish
The anticonvulsive potential of proteins extracted from <em>Orthosiphon stamineus</em> leaves (OSLP) has never been elucidated in zebrafish (<em>Danio rerio</em>). This study thus aims to elucidate the anticonvulsive potential of OSLP in pentylenetetrazol (PTZ)-induced seizur...
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doaj-0c22607e74de4a8b8237cafdb650d0332020-11-25T03:34:45ZengMDPI AGBiomedicines2227-90592020-07-01819119110.3390/biomedicines8070191<i>Orthosiphon stamineus</i> Proteins Alleviate Pentylenetetrazol-Induced Seizures in ZebrafishYin-Sir Chung0Brandon Kar Meng Choo1Pervaiz Khalid Ahmed2Iekhsan Othman3Mohd. Farooq Shaikh4Neuropharmacology Research Laboratory, Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Bandar Sunway 47500, MalaysiaNeuropharmacology Research Laboratory, Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Bandar Sunway 47500, MalaysiaSchool of Business, Monash University Malaysia, Bandar Sunway 47500, MalaysiaNeuropharmacology Research Laboratory, Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Bandar Sunway 47500, MalaysiaNeuropharmacology Research Laboratory, Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Bandar Sunway 47500, MalaysiaThe anticonvulsive potential of proteins extracted from <em>Orthosiphon stamineus</em> leaves (OSLP) has never been elucidated in zebrafish (<em>Danio rerio</em>). This study thus aims to elucidate the anticonvulsive potential of OSLP in pentylenetetrazol (PTZ)-induced seizure model. Physical changes (seizure score and seizure onset time, behavior, locomotor) and neurotransmitter analysis were elucidated to assess the pharmacological activity. The protective mechanism of OSLP on brain was also studied using mass spectrometry-based label-free proteomic quantification (LFQ) and bioinformatics. OSLP was found to be safe up to 800 µg/kg and pre-treatment with OSLP (800 µg/kg, i.p., 30 min) decreased the frequency of convulsive activities (lower seizure score and prolonged seizure onset time), improved locomotor behaviors (reduced erratic swimming movements and bottom-dwelling habit), and lowered the excitatory neurotransmitter (glutamate). Pre-treatment with OSLP increased protein Complexin 2 (Cplx 2) expression in the zebrafish brain. Cplx2 is an important regulator in the trans-SNARE complex which is required during the vesicle priming phase in the calcium-dependent synaptic vesicle exocytosis. Findings in this study collectively suggests that OSLP could be regulating the release of neurotransmitters via calcium-dependent synaptic vesicle exocytosis mediated by the “Synaptic Vesicle Cycle” pathway. OSLP’s anticonvulsive actions could be acting differently from diazepam (DZP) and with that, it might not produce the similar cognitive insults such as DZP.https://www.mdpi.com/2227-9059/8/7/191<i>Orthosiphon stamineus</i>plant-derived proteinsepilepsyseizureszebrafish |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yin-Sir Chung Brandon Kar Meng Choo Pervaiz Khalid Ahmed Iekhsan Othman Mohd. Farooq Shaikh |
spellingShingle |
Yin-Sir Chung Brandon Kar Meng Choo Pervaiz Khalid Ahmed Iekhsan Othman Mohd. Farooq Shaikh <i>Orthosiphon stamineus</i> Proteins Alleviate Pentylenetetrazol-Induced Seizures in Zebrafish Biomedicines <i>Orthosiphon stamineus</i> plant-derived proteins epilepsy seizures zebrafish |
author_facet |
Yin-Sir Chung Brandon Kar Meng Choo Pervaiz Khalid Ahmed Iekhsan Othman Mohd. Farooq Shaikh |
author_sort |
Yin-Sir Chung |
title |
<i>Orthosiphon stamineus</i> Proteins Alleviate Pentylenetetrazol-Induced Seizures in Zebrafish |
title_short |
<i>Orthosiphon stamineus</i> Proteins Alleviate Pentylenetetrazol-Induced Seizures in Zebrafish |
title_full |
<i>Orthosiphon stamineus</i> Proteins Alleviate Pentylenetetrazol-Induced Seizures in Zebrafish |
title_fullStr |
<i>Orthosiphon stamineus</i> Proteins Alleviate Pentylenetetrazol-Induced Seizures in Zebrafish |
title_full_unstemmed |
<i>Orthosiphon stamineus</i> Proteins Alleviate Pentylenetetrazol-Induced Seizures in Zebrafish |
title_sort |
<i>orthosiphon stamineus</i> proteins alleviate pentylenetetrazol-induced seizures in zebrafish |
publisher |
MDPI AG |
series |
Biomedicines |
issn |
2227-9059 |
publishDate |
2020-07-01 |
description |
The anticonvulsive potential of proteins extracted from <em>Orthosiphon stamineus</em> leaves (OSLP) has never been elucidated in zebrafish (<em>Danio rerio</em>). This study thus aims to elucidate the anticonvulsive potential of OSLP in pentylenetetrazol (PTZ)-induced seizure model. Physical changes (seizure score and seizure onset time, behavior, locomotor) and neurotransmitter analysis were elucidated to assess the pharmacological activity. The protective mechanism of OSLP on brain was also studied using mass spectrometry-based label-free proteomic quantification (LFQ) and bioinformatics. OSLP was found to be safe up to 800 µg/kg and pre-treatment with OSLP (800 µg/kg, i.p., 30 min) decreased the frequency of convulsive activities (lower seizure score and prolonged seizure onset time), improved locomotor behaviors (reduced erratic swimming movements and bottom-dwelling habit), and lowered the excitatory neurotransmitter (glutamate). Pre-treatment with OSLP increased protein Complexin 2 (Cplx 2) expression in the zebrafish brain. Cplx2 is an important regulator in the trans-SNARE complex which is required during the vesicle priming phase in the calcium-dependent synaptic vesicle exocytosis. Findings in this study collectively suggests that OSLP could be regulating the release of neurotransmitters via calcium-dependent synaptic vesicle exocytosis mediated by the “Synaptic Vesicle Cycle” pathway. OSLP’s anticonvulsive actions could be acting differently from diazepam (DZP) and with that, it might not produce the similar cognitive insults such as DZP. |
topic |
<i>Orthosiphon stamineus</i> plant-derived proteins epilepsy seizures zebrafish |
url |
https://www.mdpi.com/2227-9059/8/7/191 |
work_keys_str_mv |
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