Renal auto-transplantation promotes cortical microvascular network remodeling in a preclinical porcine model.
The vascular network is a major target of ischemia-reperfusion, but has been poorly investigated in renal transplantation. The aim of this study was to characterize the remodeling of the renal vascular network that follows ischemia-reperfusion along with the most highly affected cortex section in a...
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doaj-0c31cd2c956544c481ef96d7ac4dcdca2020-11-25T01:45:50ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01127e018106710.1371/journal.pone.0181067Renal auto-transplantation promotes cortical microvascular network remodeling in a preclinical porcine model.Souleymane MaïgaGeraldine AllainThierry HauetJerome RoumyEdouard BaulierMichel ScepiManuel DierickLuc Van HoorebekePatrick HannaertFranck GuyFrederic FavreauThe vascular network is a major target of ischemia-reperfusion, but has been poorly investigated in renal transplantation. The aim of this study was to characterize the remodeling of the renal vascular network that follows ischemia-reperfusion along with the most highly affected cortex section in a preclinical renal transplantation model. There were two experimental groups. The first was a grafted kidney group consisting of large white pigs for which the left kidney was harvested, cold flushed, preserved for 24 h in the University of Wisconsin's preservation solution, and then auto-transplanted (n = 5); the right kidney was removed to mimic the situation of human kidney transplantation. The second group (uni-nephrectomized kidney group) consisted of animals that underwent only right nephrectomy, but not left renal transplantation (n = 5). Three months after autotransplantation, the kidneys were studied by X-ray microcomputed tomography. Vessel morphology and density and tortuosity of the network were analyzed using a 3D image analysis method. Cortical blood flow was determined by laser doppler analysis and renal function and tissue injury assessed by plasma creatinine levels and histological analysis. Renal ischemia-reperfusion led to decreased vascular segment volume associated with fewer vessels of less than 30 μm, particularly in the inner cortex:0.79 ± 0.54% in grafted kidneys vs. 7.06 ± 1.44% in uni-nephrectomized kidneys, p < 0.05. Vessels showed higher connectivity throughout the cortex (the arborescence factor of the whole cortex was less in grafted than uni-nephrectomized kidneys 0.90 ± 0.04 vs. 1.07 ± 0.05, p < 0.05, with an increase in the number of bifurcations). Furthermore, cortical blood flow decreased early in kidney grafts and remained low three months after auto-transplantation. The decrease in microvasculature correlated with a deterioration of renal function, proteinuria, and tubular dysfunction, and was associated with the development of fibrous tissue. This work provides new evidence concerning the impact of ischemia-reperfusion injuries on the spectrum of renal vascular diseases and could potentially guide future therapy to preserve microvessels in transplantation ischemia-reperfusion injury.http://europepmc.org/articles/PMC5509304?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Souleymane Maïga Geraldine Allain Thierry Hauet Jerome Roumy Edouard Baulier Michel Scepi Manuel Dierick Luc Van Hoorebeke Patrick Hannaert Franck Guy Frederic Favreau |
spellingShingle |
Souleymane Maïga Geraldine Allain Thierry Hauet Jerome Roumy Edouard Baulier Michel Scepi Manuel Dierick Luc Van Hoorebeke Patrick Hannaert Franck Guy Frederic Favreau Renal auto-transplantation promotes cortical microvascular network remodeling in a preclinical porcine model. PLoS ONE |
author_facet |
Souleymane Maïga Geraldine Allain Thierry Hauet Jerome Roumy Edouard Baulier Michel Scepi Manuel Dierick Luc Van Hoorebeke Patrick Hannaert Franck Guy Frederic Favreau |
author_sort |
Souleymane Maïga |
title |
Renal auto-transplantation promotes cortical microvascular network remodeling in a preclinical porcine model. |
title_short |
Renal auto-transplantation promotes cortical microvascular network remodeling in a preclinical porcine model. |
title_full |
Renal auto-transplantation promotes cortical microvascular network remodeling in a preclinical porcine model. |
title_fullStr |
Renal auto-transplantation promotes cortical microvascular network remodeling in a preclinical porcine model. |
title_full_unstemmed |
Renal auto-transplantation promotes cortical microvascular network remodeling in a preclinical porcine model. |
title_sort |
renal auto-transplantation promotes cortical microvascular network remodeling in a preclinical porcine model. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2017-01-01 |
description |
The vascular network is a major target of ischemia-reperfusion, but has been poorly investigated in renal transplantation. The aim of this study was to characterize the remodeling of the renal vascular network that follows ischemia-reperfusion along with the most highly affected cortex section in a preclinical renal transplantation model. There were two experimental groups. The first was a grafted kidney group consisting of large white pigs for which the left kidney was harvested, cold flushed, preserved for 24 h in the University of Wisconsin's preservation solution, and then auto-transplanted (n = 5); the right kidney was removed to mimic the situation of human kidney transplantation. The second group (uni-nephrectomized kidney group) consisted of animals that underwent only right nephrectomy, but not left renal transplantation (n = 5). Three months after autotransplantation, the kidneys were studied by X-ray microcomputed tomography. Vessel morphology and density and tortuosity of the network were analyzed using a 3D image analysis method. Cortical blood flow was determined by laser doppler analysis and renal function and tissue injury assessed by plasma creatinine levels and histological analysis. Renal ischemia-reperfusion led to decreased vascular segment volume associated with fewer vessels of less than 30 μm, particularly in the inner cortex:0.79 ± 0.54% in grafted kidneys vs. 7.06 ± 1.44% in uni-nephrectomized kidneys, p < 0.05. Vessels showed higher connectivity throughout the cortex (the arborescence factor of the whole cortex was less in grafted than uni-nephrectomized kidneys 0.90 ± 0.04 vs. 1.07 ± 0.05, p < 0.05, with an increase in the number of bifurcations). Furthermore, cortical blood flow decreased early in kidney grafts and remained low three months after auto-transplantation. The decrease in microvasculature correlated with a deterioration of renal function, proteinuria, and tubular dysfunction, and was associated with the development of fibrous tissue. This work provides new evidence concerning the impact of ischemia-reperfusion injuries on the spectrum of renal vascular diseases and could potentially guide future therapy to preserve microvessels in transplantation ischemia-reperfusion injury. |
url |
http://europepmc.org/articles/PMC5509304?pdf=render |
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