Preliminary Study and Improve the Production of Metabolites with Antifungal Activity by A Bacillus Sp Strain IBA 33
Bacillus sp strain IBA 33 metabolites, isolated from decaying lemon fruits, were evaluated for the control of pathogenic and non-pathogenic fungi (Penicillium digitatum, Geotrichum candidum, Penicillium expansum, Aspergillus clavatus, Aspergillus flavus, Aspergillus niger, and Fusarium moniliforme)....
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2009-01-01
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doaj-0c388aa595d4412c9f65aac21fd4b4492020-11-25T03:41:42ZengSAGE PublishingMicrobiology Insights1178-63612009-01-0121524Preliminary Study and Improve the Production of Metabolites with Antifungal Activity by A Bacillus Sp Strain IBA 33María Antonieta GordilloAntonio Roberto NavarroLidia María BenitezMarta Inés Torres de PlazaCristina MaldonadoBacillus sp strain IBA 33 metabolites, isolated from decaying lemon fruits, were evaluated for the control of pathogenic and non-pathogenic fungi (Penicillium digitatum, Geotrichum candidum, Penicillium expansum, Aspergillus clavatus, Aspergillus flavus, Aspergillus niger, and Fusarium moniliforme). These metabolites were recovered from Landy medium (LM) without aminoacids. In order to optimize metabolites production the LM was modified by adding different concentrations and sources of amino acids and carbohydrates at different culture conditions.Bacillus sp strain IBA 33 metabolites efficacy to control fungi were evaluated with in vitro and in vivo assays. A. flavus growth inhibition was 52% with the metabolites of Bacillus sp strain IBA 33 recovered from LM (MBLM) in vitro assays. MBLM supplemented with 0.5% glutamic acid, inhibited the growth of P. digitatum, G. candidum, A. clavatus, A. niger and F. moniliforme by 65%, 88.44%, 84%, 34% and 92% respectively. The highest inhibition of P. expansum was 45% with MBLM supplemented with 0.5% aspartic acid. Similar results were obtained in vivo assays. These results showed that Bacillus sp strain IBA 33 metabolites specificity against fungi depended on the composition of the LM.http://www.la-press.com/preliminary-study-and-improve-the-production-of-metabolites-with-antif-a1405biological controllemon fruitspathogenic and non-pathogenic fungi |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
María Antonieta Gordillo Antonio Roberto Navarro Lidia María Benitez Marta Inés Torres de Plaza Cristina Maldonado |
spellingShingle |
María Antonieta Gordillo Antonio Roberto Navarro Lidia María Benitez Marta Inés Torres de Plaza Cristina Maldonado Preliminary Study and Improve the Production of Metabolites with Antifungal Activity by A Bacillus Sp Strain IBA 33 Microbiology Insights biological control lemon fruits pathogenic and non-pathogenic fungi |
author_facet |
María Antonieta Gordillo Antonio Roberto Navarro Lidia María Benitez Marta Inés Torres de Plaza Cristina Maldonado |
author_sort |
María Antonieta Gordillo |
title |
Preliminary Study and Improve the Production of Metabolites with Antifungal Activity by A Bacillus Sp Strain IBA 33 |
title_short |
Preliminary Study and Improve the Production of Metabolites with Antifungal Activity by A Bacillus Sp Strain IBA 33 |
title_full |
Preliminary Study and Improve the Production of Metabolites with Antifungal Activity by A Bacillus Sp Strain IBA 33 |
title_fullStr |
Preliminary Study and Improve the Production of Metabolites with Antifungal Activity by A Bacillus Sp Strain IBA 33 |
title_full_unstemmed |
Preliminary Study and Improve the Production of Metabolites with Antifungal Activity by A Bacillus Sp Strain IBA 33 |
title_sort |
preliminary study and improve the production of metabolites with antifungal activity by a bacillus sp strain iba 33 |
publisher |
SAGE Publishing |
series |
Microbiology Insights |
issn |
1178-6361 |
publishDate |
2009-01-01 |
description |
Bacillus sp strain IBA 33 metabolites, isolated from decaying lemon fruits, were evaluated for the control of pathogenic and non-pathogenic fungi (Penicillium digitatum, Geotrichum candidum, Penicillium expansum, Aspergillus clavatus, Aspergillus flavus, Aspergillus niger, and Fusarium moniliforme). These metabolites were recovered from Landy medium (LM) without aminoacids. In order to optimize metabolites production the LM was modified by adding different concentrations and sources of amino acids and carbohydrates at different culture conditions.Bacillus sp strain IBA 33 metabolites efficacy to control fungi were evaluated with in vitro and in vivo assays. A. flavus growth inhibition was 52% with the metabolites of Bacillus sp strain IBA 33 recovered from LM (MBLM) in vitro assays. MBLM supplemented with 0.5% glutamic acid, inhibited the growth of P. digitatum, G. candidum, A. clavatus, A. niger and F. moniliforme by 65%, 88.44%, 84%, 34% and 92% respectively. The highest inhibition of P. expansum was 45% with MBLM supplemented with 0.5% aspartic acid. Similar results were obtained in vivo assays. These results showed that Bacillus sp strain IBA 33 metabolites specificity against fungi depended on the composition of the LM. |
topic |
biological control lemon fruits pathogenic and non-pathogenic fungi |
url |
http://www.la-press.com/preliminary-study-and-improve-the-production-of-metabolites-with-antif-a1405 |
work_keys_str_mv |
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