Preliminary Study and Improve the Production of Metabolites with Antifungal Activity by A Bacillus Sp Strain IBA 33

Bacillus sp strain IBA 33 metabolites, isolated from decaying lemon fruits, were evaluated for the control of pathogenic and non-pathogenic fungi (Penicillium digitatum, Geotrichum candidum, Penicillium expansum, Aspergillus clavatus, Aspergillus flavus, Aspergillus niger, and Fusarium moniliforme)....

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Main Authors: María Antonieta Gordillo, Antonio Roberto Navarro, Lidia María Benitez, Marta Inés Torres de Plaza, Cristina Maldonado
Format: Article
Language:English
Published: SAGE Publishing 2009-01-01
Series:Microbiology Insights
Subjects:
Online Access:http://www.la-press.com/preliminary-study-and-improve-the-production-of-metabolites-with-antif-a1405
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spelling doaj-0c388aa595d4412c9f65aac21fd4b4492020-11-25T03:41:42ZengSAGE PublishingMicrobiology Insights1178-63612009-01-0121524Preliminary Study and Improve the Production of Metabolites with Antifungal Activity by A Bacillus Sp Strain IBA 33María Antonieta GordilloAntonio Roberto NavarroLidia María BenitezMarta Inés Torres de PlazaCristina MaldonadoBacillus sp strain IBA 33 metabolites, isolated from decaying lemon fruits, were evaluated for the control of pathogenic and non-pathogenic fungi (Penicillium digitatum, Geotrichum candidum, Penicillium expansum, Aspergillus clavatus, Aspergillus flavus, Aspergillus niger, and Fusarium moniliforme). These metabolites were recovered from Landy medium (LM) without aminoacids. In order to optimize metabolites production the LM was modified by adding different concentrations and sources of amino acids and carbohydrates at different culture conditions.Bacillus sp strain IBA 33 metabolites efficacy to control fungi were evaluated with in vitro and in vivo assays. A. flavus growth inhibition was 52% with the metabolites of Bacillus sp strain IBA 33 recovered from LM (MBLM) in vitro assays. MBLM supplemented with 0.5% glutamic acid, inhibited the growth of P. digitatum, G. candidum, A. clavatus, A. niger and F. moniliforme by 65%, 88.44%, 84%, 34% and 92% respectively. The highest inhibition of P. expansum was 45% with MBLM supplemented with 0.5% aspartic acid. Similar results were obtained in vivo assays. These results showed that Bacillus sp strain IBA 33 metabolites specificity against fungi depended on the composition of the LM.http://www.la-press.com/preliminary-study-and-improve-the-production-of-metabolites-with-antif-a1405biological controllemon fruitspathogenic and non-pathogenic fungi
collection DOAJ
language English
format Article
sources DOAJ
author María Antonieta Gordillo
Antonio Roberto Navarro
Lidia María Benitez
Marta Inés Torres de Plaza
Cristina Maldonado
spellingShingle María Antonieta Gordillo
Antonio Roberto Navarro
Lidia María Benitez
Marta Inés Torres de Plaza
Cristina Maldonado
Preliminary Study and Improve the Production of Metabolites with Antifungal Activity by A Bacillus Sp Strain IBA 33
Microbiology Insights
biological control
lemon fruits
pathogenic and non-pathogenic fungi
author_facet María Antonieta Gordillo
Antonio Roberto Navarro
Lidia María Benitez
Marta Inés Torres de Plaza
Cristina Maldonado
author_sort María Antonieta Gordillo
title Preliminary Study and Improve the Production of Metabolites with Antifungal Activity by A Bacillus Sp Strain IBA 33
title_short Preliminary Study and Improve the Production of Metabolites with Antifungal Activity by A Bacillus Sp Strain IBA 33
title_full Preliminary Study and Improve the Production of Metabolites with Antifungal Activity by A Bacillus Sp Strain IBA 33
title_fullStr Preliminary Study and Improve the Production of Metabolites with Antifungal Activity by A Bacillus Sp Strain IBA 33
title_full_unstemmed Preliminary Study and Improve the Production of Metabolites with Antifungal Activity by A Bacillus Sp Strain IBA 33
title_sort preliminary study and improve the production of metabolites with antifungal activity by a bacillus sp strain iba 33
publisher SAGE Publishing
series Microbiology Insights
issn 1178-6361
publishDate 2009-01-01
description Bacillus sp strain IBA 33 metabolites, isolated from decaying lemon fruits, were evaluated for the control of pathogenic and non-pathogenic fungi (Penicillium digitatum, Geotrichum candidum, Penicillium expansum, Aspergillus clavatus, Aspergillus flavus, Aspergillus niger, and Fusarium moniliforme). These metabolites were recovered from Landy medium (LM) without aminoacids. In order to optimize metabolites production the LM was modified by adding different concentrations and sources of amino acids and carbohydrates at different culture conditions.Bacillus sp strain IBA 33 metabolites efficacy to control fungi were evaluated with in vitro and in vivo assays. A. flavus growth inhibition was 52% with the metabolites of Bacillus sp strain IBA 33 recovered from LM (MBLM) in vitro assays. MBLM supplemented with 0.5% glutamic acid, inhibited the growth of P. digitatum, G. candidum, A. clavatus, A. niger and F. moniliforme by 65%, 88.44%, 84%, 34% and 92% respectively. The highest inhibition of P. expansum was 45% with MBLM supplemented with 0.5% aspartic acid. Similar results were obtained in vivo assays. These results showed that Bacillus sp strain IBA 33 metabolites specificity against fungi depended on the composition of the LM.
topic biological control
lemon fruits
pathogenic and non-pathogenic fungi
url http://www.la-press.com/preliminary-study-and-improve-the-production-of-metabolites-with-antif-a1405
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