Proteomic profiling of archaeological human bone

Ancient protein analysis provides clues to human life and diseases from ancient times. Here, we performed shotgun proteomics of human archeological bones for the first time, using rib bones from the Hitotsubashi site (AD 1657–1683) in Tokyo, called Edo in ancient times. The output data obtained were...

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Main Authors: Rikai Sawafuji, Enrico Cappellini, Tomohito Nagaoka, Anna K. Fotakis, Rosa Rakownikow Jersie-Christensen, Jesper V. Olsen, Kazuaki Hirata, Shintaroh Ueda
Format: Article
Language:English
Published: The Royal Society 2017-01-01
Series:Royal Society Open Science
Subjects:
Online Access:https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.161004
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spelling doaj-0c3c0b7c22b3488eb4f31de0d69290232020-11-25T04:02:57ZengThe Royal SocietyRoyal Society Open Science2054-57032017-01-014610.1098/rsos.161004161004Proteomic profiling of archaeological human boneRikai SawafujiEnrico CappelliniTomohito NagaokaAnna K. FotakisRosa Rakownikow Jersie-ChristensenJesper V. OlsenKazuaki HirataShintaroh UedaAncient protein analysis provides clues to human life and diseases from ancient times. Here, we performed shotgun proteomics of human archeological bones for the first time, using rib bones from the Hitotsubashi site (AD 1657–1683) in Tokyo, called Edo in ancient times. The output data obtained were analysed using Gene Ontology and label-free quantification. We detected leucocyte-derived proteins, possibly originating from the bone marrow of the rib. Particularly prevalent and relatively high expression of eosinophil peroxidase suggests the influence of infectious diseases. This scenario is plausible, considering the overcrowding and unhygienic living conditions of the Edo city described in the historical literature. We also observed age-dependent differences in proteome profiles, particularly for proteins involved in developmental processes. Among them, alpha-2-HS-glycoprotein demonstrated a strong negative correlation with age. These results suggest that analysis of ancient proteins could provide a useful indicator of stress, disease, starvation, obesity and other kinds of physiological and pathological information.https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.161004proteomicsimmune systemage-related changesarchaeological bonemass spectrometry
collection DOAJ
language English
format Article
sources DOAJ
author Rikai Sawafuji
Enrico Cappellini
Tomohito Nagaoka
Anna K. Fotakis
Rosa Rakownikow Jersie-Christensen
Jesper V. Olsen
Kazuaki Hirata
Shintaroh Ueda
spellingShingle Rikai Sawafuji
Enrico Cappellini
Tomohito Nagaoka
Anna K. Fotakis
Rosa Rakownikow Jersie-Christensen
Jesper V. Olsen
Kazuaki Hirata
Shintaroh Ueda
Proteomic profiling of archaeological human bone
Royal Society Open Science
proteomics
immune system
age-related changes
archaeological bone
mass spectrometry
author_facet Rikai Sawafuji
Enrico Cappellini
Tomohito Nagaoka
Anna K. Fotakis
Rosa Rakownikow Jersie-Christensen
Jesper V. Olsen
Kazuaki Hirata
Shintaroh Ueda
author_sort Rikai Sawafuji
title Proteomic profiling of archaeological human bone
title_short Proteomic profiling of archaeological human bone
title_full Proteomic profiling of archaeological human bone
title_fullStr Proteomic profiling of archaeological human bone
title_full_unstemmed Proteomic profiling of archaeological human bone
title_sort proteomic profiling of archaeological human bone
publisher The Royal Society
series Royal Society Open Science
issn 2054-5703
publishDate 2017-01-01
description Ancient protein analysis provides clues to human life and diseases from ancient times. Here, we performed shotgun proteomics of human archeological bones for the first time, using rib bones from the Hitotsubashi site (AD 1657–1683) in Tokyo, called Edo in ancient times. The output data obtained were analysed using Gene Ontology and label-free quantification. We detected leucocyte-derived proteins, possibly originating from the bone marrow of the rib. Particularly prevalent and relatively high expression of eosinophil peroxidase suggests the influence of infectious diseases. This scenario is plausible, considering the overcrowding and unhygienic living conditions of the Edo city described in the historical literature. We also observed age-dependent differences in proteome profiles, particularly for proteins involved in developmental processes. Among them, alpha-2-HS-glycoprotein demonstrated a strong negative correlation with age. These results suggest that analysis of ancient proteins could provide a useful indicator of stress, disease, starvation, obesity and other kinds of physiological and pathological information.
topic proteomics
immune system
age-related changes
archaeological bone
mass spectrometry
url https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.161004
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