Cobalamin is present in cells of non-tuberculous mycobacteria, but not in Mycobacterium tuberculosis
Abstract Cobalamin (vitamin B12) is a structurally complex molecule that acts as a cofactor for enzymes and regulates gene expression through so-called riboswitches. The existing literature on the vitamin B12 synthesis capacity in Mycobacterium tuberculosis is ambiguous, while in non-tuberculous myc...
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2021-06-01
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doaj-0c4eaab9d12343ddbd3253ad0c70cd112021-06-13T11:40:48ZengNature Publishing GroupScientific Reports2045-23222021-06-0111111910.1038/s41598-021-91430-wCobalamin is present in cells of non-tuberculous mycobacteria, but not in Mycobacterium tuberculosisAlina Minias0Filip Gąsior1Anna Brzostek2Tomasz Jagielski3Jarosław Dziadek4Laboratory of Genetics and Physiology of Mycobacterium, Institute of Medical Biology, Polish Academy of SciencesLaboratory of Genetics and Physiology of Mycobacterium, Institute of Medical Biology, Polish Academy of SciencesLaboratory of Genetics and Physiology of Mycobacterium, Institute of Medical Biology, Polish Academy of SciencesDepartment of Medical Microbiology, Institute of Microbiology, Faculty of Biology, University of WarsawLaboratory of Genetics and Physiology of Mycobacterium, Institute of Medical Biology, Polish Academy of SciencesAbstract Cobalamin (vitamin B12) is a structurally complex molecule that acts as a cofactor for enzymes and regulates gene expression through so-called riboswitches. The existing literature on the vitamin B12 synthesis capacity in Mycobacterium tuberculosis is ambiguous, while in non-tuberculous mycobacteria (NTM) is rather marginal. Here we present the results of our investigation into the occurrence of vitamin B12 in mycobacteria. For detection purposes, immunoassay methods were applied to cell lysates of NTM and M. tuberculosis clinical and laboratory strains grown under different conditions. We show that whereas vitamin B12 is present in cells of various NTM species, it cannot be evidenced in strains of differently cultured M. tuberculosis, even though the genes responsible for vitamin B12 synthesis are actively expressed based on RNA-Seq data. In summary, we conclude that the production of vitamin B12 does occur in mycobacteria, with the likely exception of M. tuberculosis. Our results provide direct evidence of vitamin B12 synthesis in a clinically important group of bacteria.https://doi.org/10.1038/s41598-021-91430-w |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alina Minias Filip Gąsior Anna Brzostek Tomasz Jagielski Jarosław Dziadek |
spellingShingle |
Alina Minias Filip Gąsior Anna Brzostek Tomasz Jagielski Jarosław Dziadek Cobalamin is present in cells of non-tuberculous mycobacteria, but not in Mycobacterium tuberculosis Scientific Reports |
author_facet |
Alina Minias Filip Gąsior Anna Brzostek Tomasz Jagielski Jarosław Dziadek |
author_sort |
Alina Minias |
title |
Cobalamin is present in cells of non-tuberculous mycobacteria, but not in Mycobacterium tuberculosis |
title_short |
Cobalamin is present in cells of non-tuberculous mycobacteria, but not in Mycobacterium tuberculosis |
title_full |
Cobalamin is present in cells of non-tuberculous mycobacteria, but not in Mycobacterium tuberculosis |
title_fullStr |
Cobalamin is present in cells of non-tuberculous mycobacteria, but not in Mycobacterium tuberculosis |
title_full_unstemmed |
Cobalamin is present in cells of non-tuberculous mycobacteria, but not in Mycobacterium tuberculosis |
title_sort |
cobalamin is present in cells of non-tuberculous mycobacteria, but not in mycobacterium tuberculosis |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2021-06-01 |
description |
Abstract Cobalamin (vitamin B12) is a structurally complex molecule that acts as a cofactor for enzymes and regulates gene expression through so-called riboswitches. The existing literature on the vitamin B12 synthesis capacity in Mycobacterium tuberculosis is ambiguous, while in non-tuberculous mycobacteria (NTM) is rather marginal. Here we present the results of our investigation into the occurrence of vitamin B12 in mycobacteria. For detection purposes, immunoassay methods were applied to cell lysates of NTM and M. tuberculosis clinical and laboratory strains grown under different conditions. We show that whereas vitamin B12 is present in cells of various NTM species, it cannot be evidenced in strains of differently cultured M. tuberculosis, even though the genes responsible for vitamin B12 synthesis are actively expressed based on RNA-Seq data. In summary, we conclude that the production of vitamin B12 does occur in mycobacteria, with the likely exception of M. tuberculosis. Our results provide direct evidence of vitamin B12 synthesis in a clinically important group of bacteria. |
url |
https://doi.org/10.1038/s41598-021-91430-w |
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