Definitive Endoderm Formation from Plucked Human Hair-Derived Induced Pluripotent Stem Cells and SK Channel Regulation
Pluripotent stem cells present an extraordinary powerful tool to investigate embryonic development in humans. Essentially, they provide a unique platform for dissecting the distinct mechanisms underlying pluripotency and subsequent lineage commitment. Modest information currently exists about the ex...
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doaj-0c4f390c577649219ddc6b4233e5b8332020-11-24T21:56:02ZengHindawi LimitedStem Cells International1687-966X1687-96782013-01-01201310.1155/2013/360573360573Definitive Endoderm Formation from Plucked Human Hair-Derived Induced Pluripotent Stem Cells and SK Channel RegulationAnett Illing0Marianne Stockmann1Narasimha Swamy Telugu2Leonhard Linta3Ronan Russell4Martin Müller5Thomas Seufferlein6Stefan Liebau7Alexander Kleger8 Department of Internal Medicine I, University of Ulm, Albert-Einstein Allee 23, 89081 Ulm, Germany Department of Internal Medicine I, University of Ulm, Albert-Einstein Allee 23, 89081 Ulm, Germany Department of Internal Medicine I, University of Ulm, Albert-Einstein Allee 23, 89081 Ulm, GermanyInstitute for Anatomy and Cell Biology, University of Ulm, Albert-Einstein Allee 11, 89081 Ulm, Germany Department of Internal Medicine I, University of Ulm, Albert-Einstein Allee 23, 89081 Ulm, Germany Department of Internal Medicine I, University of Ulm, Albert-Einstein Allee 23, 89081 Ulm, Germany Department of Internal Medicine I, University of Ulm, Albert-Einstein Allee 23, 89081 Ulm, GermanyInstitute for Anatomy and Cell Biology, University of Ulm, Albert-Einstein Allee 11, 89081 Ulm, Germany Department of Internal Medicine I, University of Ulm, Albert-Einstein Allee 23, 89081 Ulm, GermanyPluripotent stem cells present an extraordinary powerful tool to investigate embryonic development in humans. Essentially, they provide a unique platform for dissecting the distinct mechanisms underlying pluripotency and subsequent lineage commitment. Modest information currently exists about the expression and the role of ion channels during human embryogenesis, organ development, and cell fate determination. Of note, small and intermediate conductance, calcium-activated potassium channels have been reported to modify stem cell behaviour and differentiation. These channels are broadly expressed throughout human tissues and are involved in various cellular processes, such as the after-hyperpolarization in excitable cells, and also in differentiation processes. To this end, human induced pluripotent stem cells (hiPSCs) generated from plucked human hair keratinocytes have been exploited in vitro to recapitulate endoderm formation and, concomitantly, used to map the expression of the SK channel (SKCa) subtypes over time. Thus, we report the successful generation of definitive endoderm from hiPSCs of ectodermal origin using a highly reproducible and robust differentiation system. Furthermore, we provide the first evidence that SKCas subtypes are dynamically regulated in the transition from a pluripotent stem cell to a more lineage restricted, endodermal progeny.http://dx.doi.org/10.1155/2013/360573 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Anett Illing Marianne Stockmann Narasimha Swamy Telugu Leonhard Linta Ronan Russell Martin Müller Thomas Seufferlein Stefan Liebau Alexander Kleger |
spellingShingle |
Anett Illing Marianne Stockmann Narasimha Swamy Telugu Leonhard Linta Ronan Russell Martin Müller Thomas Seufferlein Stefan Liebau Alexander Kleger Definitive Endoderm Formation from Plucked Human Hair-Derived Induced Pluripotent Stem Cells and SK Channel Regulation Stem Cells International |
author_facet |
Anett Illing Marianne Stockmann Narasimha Swamy Telugu Leonhard Linta Ronan Russell Martin Müller Thomas Seufferlein Stefan Liebau Alexander Kleger |
author_sort |
Anett Illing |
title |
Definitive Endoderm Formation from Plucked Human Hair-Derived Induced Pluripotent Stem Cells and SK Channel Regulation |
title_short |
Definitive Endoderm Formation from Plucked Human Hair-Derived Induced Pluripotent Stem Cells and SK Channel Regulation |
title_full |
Definitive Endoderm Formation from Plucked Human Hair-Derived Induced Pluripotent Stem Cells and SK Channel Regulation |
title_fullStr |
Definitive Endoderm Formation from Plucked Human Hair-Derived Induced Pluripotent Stem Cells and SK Channel Regulation |
title_full_unstemmed |
Definitive Endoderm Formation from Plucked Human Hair-Derived Induced Pluripotent Stem Cells and SK Channel Regulation |
title_sort |
definitive endoderm formation from plucked human hair-derived induced pluripotent stem cells and sk channel regulation |
publisher |
Hindawi Limited |
series |
Stem Cells International |
issn |
1687-966X 1687-9678 |
publishDate |
2013-01-01 |
description |
Pluripotent stem cells present an extraordinary powerful tool to investigate embryonic development in humans. Essentially, they provide a unique platform for dissecting the distinct mechanisms underlying pluripotency and subsequent lineage commitment. Modest information currently exists about the expression and the role of ion channels during human embryogenesis, organ development, and cell fate determination. Of note, small and intermediate conductance, calcium-activated potassium channels have been reported to modify stem cell behaviour and differentiation. These channels are broadly expressed throughout human tissues and are involved in various cellular processes, such as the after-hyperpolarization in excitable cells, and also in differentiation processes. To this end, human induced pluripotent stem cells (hiPSCs) generated from plucked human hair keratinocytes have been exploited in vitro to recapitulate endoderm formation and, concomitantly, used to map the expression of the SK channel (SKCa) subtypes over time. Thus, we report the successful generation of definitive endoderm from hiPSCs of ectodermal origin using a highly reproducible and robust differentiation system. Furthermore, we provide the first evidence that SKCas subtypes are dynamically regulated in the transition from a pluripotent stem cell to a more lineage restricted, endodermal progeny. |
url |
http://dx.doi.org/10.1155/2013/360573 |
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