Protective Effect of Gwakhyangjeonggisan Herbal Acupuncture Solution in Glioblastoma Cells: Microarray Analysis of Gene Expression
Objectives : Neurological disorders have been one of main therapeutic targets of acupuncture. The present study investigated the protective effects of Gwakhyangjeonggisan herbal acupuncture solution (GHAS). Methods : We performed 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) a...
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Korean Pharmacopuncture Institute
2005-12-01
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Online Access: | http://dx.doi.org/10.3831/KPI.2005.8.3.057 |
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doaj-0c54672080454fc3bf090500be6d5f932020-11-25T02:37:00ZengKorean Pharmacopuncture InstituteJournal of Pharmacopuncture2093-69662234-68562005-12-0183576910.3831/KPI.2005.8.3.057 Protective Effect of Gwakhyangjeonggisan Herbal Acupuncture Solution in Glioblastoma Cells: Microarray Analysis of Gene ExpressionHong-Seok Lee0Chang-Shik Yin1Hyeong-Gyun Koh2Dept. of Acupuncture, College of Oriental Medicine, Kyung Hee UniversityDept. of Acupuncture, CHA Biomedical Center, College of Medicine, Pochon CHA UniversityDept. of Acupuncture, College of Oriental Medicine, Kyung Hee UniversityObjectives : Neurological disorders have been one of main therapeutic targets of acupuncture. The present study investigated the protective effects of Gwakhyangjeonggisan herbal acupuncture solution (GHAS). Methods : We performed 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in glioblastoma cells, and did microarray analysis with cells exposed to reactive oxigen species (ROS) of hydrogen peroxide by 8.0 k Human cDNA, with cut-off level of 2-fold changes in gene expression. Results : MTT assay showed protective effect of GHAS on the glioblastoma cells exposed to hydrogen peroxide. When glioblastoma cells were exposed to hydrogen peroxide, 24 genes were downregulated. When the cells were pretreated with GHAS before exposure to hydrogen peroxide, 46 genes were downregulated. Many of the genes downregulated by hydrogen peroxide stimulation were decreased in the amount of downregulation or reversed to upregulation. Conclusions : The gene expression changes observed in the present study are supposed to be related to the protective molecular mechanism of GHAS in the glioblastoma cells exposed to ROS stress.http://dx.doi.org/10.3831/KPI.2005.8.3.057Gwakhyangjeonggisan herbal acupuncture solution (GHAS)GlioblastomaMicroarrayGene expression |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hong-Seok Lee Chang-Shik Yin Hyeong-Gyun Koh |
spellingShingle |
Hong-Seok Lee Chang-Shik Yin Hyeong-Gyun Koh Protective Effect of Gwakhyangjeonggisan Herbal Acupuncture Solution in Glioblastoma Cells: Microarray Analysis of Gene Expression Journal of Pharmacopuncture Gwakhyangjeonggisan herbal acupuncture solution (GHAS) Glioblastoma Microarray Gene expression |
author_facet |
Hong-Seok Lee Chang-Shik Yin Hyeong-Gyun Koh |
author_sort |
Hong-Seok Lee |
title |
Protective Effect of Gwakhyangjeonggisan Herbal Acupuncture Solution in Glioblastoma Cells: Microarray Analysis of Gene Expression |
title_short |
Protective Effect of Gwakhyangjeonggisan Herbal Acupuncture Solution in Glioblastoma Cells: Microarray Analysis of Gene Expression |
title_full |
Protective Effect of Gwakhyangjeonggisan Herbal Acupuncture Solution in Glioblastoma Cells: Microarray Analysis of Gene Expression |
title_fullStr |
Protective Effect of Gwakhyangjeonggisan Herbal Acupuncture Solution in Glioblastoma Cells: Microarray Analysis of Gene Expression |
title_full_unstemmed |
Protective Effect of Gwakhyangjeonggisan Herbal Acupuncture Solution in Glioblastoma Cells: Microarray Analysis of Gene Expression |
title_sort |
protective effect of gwakhyangjeonggisan herbal acupuncture solution in glioblastoma cells: microarray analysis of gene expression |
publisher |
Korean Pharmacopuncture Institute |
series |
Journal of Pharmacopuncture |
issn |
2093-6966 2234-6856 |
publishDate |
2005-12-01 |
description |
Objectives : Neurological disorders have been one of main therapeutic targets of acupuncture. The present study investigated the protective effects of Gwakhyangjeonggisan herbal acupuncture solution (GHAS).
Methods : We performed 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in glioblastoma cells, and did microarray analysis with cells exposed to reactive oxigen species (ROS) of hydrogen peroxide by 8.0 k Human cDNA, with cut-off level of 2-fold changes in gene expression.
Results : MTT assay showed protective effect of GHAS on the glioblastoma cells exposed to hydrogen peroxide. When glioblastoma cells were exposed to hydrogen peroxide, 24 genes were downregulated. When the cells were pretreated with GHAS before exposure to hydrogen peroxide, 46 genes were downregulated. Many of the genes downregulated by hydrogen peroxide stimulation were decreased in the amount of downregulation or reversed to upregulation.
Conclusions : The gene expression changes observed in the present study are supposed to be related to the protective molecular mechanism of GHAS in the glioblastoma cells exposed to ROS stress. |
topic |
Gwakhyangjeonggisan herbal acupuncture solution (GHAS) Glioblastoma Microarray Gene expression |
url |
http://dx.doi.org/10.3831/KPI.2005.8.3.057 |
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