Glutathione Redox System in β-Thalassemia/Hb E Patients
β-thalassemia/Hb E is known to cause oxidative stress induced by iron overload. The glutathione system is the major endogenous antioxidant that protects animal cells from oxidative damage. This study aimed to determine the effect of disease state and splenectomy on redox status expressed by whole...
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doaj-0c6656f676344c118cffe4be7f872a802020-11-24T21:24:59ZengHindawi LimitedThe Scientific World Journal1537-744X2013-01-01201310.1155/2013/543973543973Glutathione Redox System in β-Thalassemia/Hb E PatientsRuchaneekorn W. Kalpravidh0Thongchai Tangjaidee1Suneerat Hatairaktham2Ratiya Charoensakdi3Narumol Panichkul4Noppadol Siritanaratkul5Suthat Fucharoen6Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, ThailandDepartment of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, ThailandDepartment of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, ThailandDepartment of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, ThailandDepartment of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, ThailandDepartment of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, ThailandThalassemia Research Center, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom 73170, Thailandβ-thalassemia/Hb E is known to cause oxidative stress induced by iron overload. The glutathione system is the major endogenous antioxidant that protects animal cells from oxidative damage. This study aimed to determine the effect of disease state and splenectomy on redox status expressed by whole blood glutathione (GSH)/glutathione disulfide (GSSG) and also to evaluate glutathione-related responses to oxidation in β-thalassemia/Hb E patients. Twenty-seven normal subjects and 25 β-thalassemia/Hb E patients were recruited and blood was collected. The GSH/GSSG ratio, activities of glutathione-related enzymes, hematological parameters, and serum ferritin levels were determined in individuals. Patients had high iron-induced oxidative stress, shown as significantly increased serum ferritin, a decreased GSH/GSSG ratio, and increased activities of glutathione-related enzymes. Splenectomy increased serum ferritin levels and decreased GSH levels concomitant with unchanged glutathione-related enzyme activities. The redox ratio had a positive correlation with hemoglobin levels and negative correlation with levels of serum ferritin. The glutathione system may be the body’s first-line defense used against oxidative stress and to maintain redox homeostasis in thalassemic patients based on the significant correlations between the GSH/GSSH ratio and degree of anemia or body iron stores.http://dx.doi.org/10.1155/2013/543973 |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ruchaneekorn W. Kalpravidh Thongchai Tangjaidee Suneerat Hatairaktham Ratiya Charoensakdi Narumol Panichkul Noppadol Siritanaratkul Suthat Fucharoen |
spellingShingle |
Ruchaneekorn W. Kalpravidh Thongchai Tangjaidee Suneerat Hatairaktham Ratiya Charoensakdi Narumol Panichkul Noppadol Siritanaratkul Suthat Fucharoen Glutathione Redox System in β-Thalassemia/Hb E Patients The Scientific World Journal |
author_facet |
Ruchaneekorn W. Kalpravidh Thongchai Tangjaidee Suneerat Hatairaktham Ratiya Charoensakdi Narumol Panichkul Noppadol Siritanaratkul Suthat Fucharoen |
author_sort |
Ruchaneekorn W. Kalpravidh |
title |
Glutathione Redox System in β-Thalassemia/Hb E Patients |
title_short |
Glutathione Redox System in β-Thalassemia/Hb E Patients |
title_full |
Glutathione Redox System in β-Thalassemia/Hb E Patients |
title_fullStr |
Glutathione Redox System in β-Thalassemia/Hb E Patients |
title_full_unstemmed |
Glutathione Redox System in β-Thalassemia/Hb E Patients |
title_sort |
glutathione redox system in β-thalassemia/hb e patients |
publisher |
Hindawi Limited |
series |
The Scientific World Journal |
issn |
1537-744X |
publishDate |
2013-01-01 |
description |
β-thalassemia/Hb E is known to cause oxidative stress induced by iron overload. The glutathione system is the major endogenous antioxidant that protects animal cells from oxidative damage. This study aimed to determine the effect of disease state and splenectomy on redox status expressed by whole blood glutathione (GSH)/glutathione disulfide (GSSG) and also to evaluate glutathione-related responses to oxidation in β-thalassemia/Hb E patients. Twenty-seven normal subjects and 25 β-thalassemia/Hb E patients were recruited and blood was collected. The GSH/GSSG ratio, activities of glutathione-related enzymes, hematological parameters, and serum ferritin levels were determined in individuals. Patients had high iron-induced oxidative stress, shown as significantly increased serum ferritin, a decreased GSH/GSSG ratio, and increased activities of glutathione-related enzymes. Splenectomy increased serum ferritin levels and decreased GSH levels concomitant with unchanged glutathione-related enzyme activities. The redox ratio had a positive correlation with hemoglobin levels and negative correlation with levels of serum ferritin. The glutathione system may be the body’s first-line defense used against oxidative stress and to maintain redox homeostasis in thalassemic patients based on the significant correlations between the GSH/GSSH ratio and degree of anemia or body iron stores. |
url |
http://dx.doi.org/10.1155/2013/543973 |
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