Polar extracts from (Tunisian) <it>Acacia salicina </it>Lindl. Study of the antimicrobial and antigenotoxic activities
<p>Abstract</p> <p>Background</p> <p>Methanolic, aqueous and Total Oligomer Flavonoids (TOF)-enriched extracts obtained from the leaves of <it>Acacia salicina </it>'Lindl.' were investigated for antibacterial, antimutagenic and antioxidant activiti...
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doaj-0c70d15b50f247a487ccec8891ad0d5d2020-11-25T02:57:29ZengBMCBMC Complementary and Alternative Medicine1472-68822012-04-011213710.1186/1472-6882-12-37Polar extracts from (Tunisian) <it>Acacia salicina </it>Lindl. Study of the antimicrobial and antigenotoxic activitiesBoubaker JihedMansour HediGhedira KamelGhedira Leila<p>Abstract</p> <p>Background</p> <p>Methanolic, aqueous and Total Oligomer Flavonoids (TOF)-enriched extracts obtained from the leaves of <it>Acacia salicina </it>'Lindl.' were investigated for antibacterial, antimutagenic and antioxidant activities.</p> <p>Methods</p> <p>The antimicrobial activity was tested on the Gram positive and Gram negative reference bacterial strains. The Mutagenic and antimutagenic activities against direct acting mutagens, methylmethane sulfonate (MMS) and 4-nitro-o-phenylenediamine (NOPD), and indirect acting mutagens, 2-aminoanthracene (2-AA) and benzo[a]pyrene (B(a)P) were performed with <it>S. typhimurium </it>TA102 and TA98 assay systems. In addition, the enzymatic and nonenzymatic methods were employed to evaluate the anti-oxidative effects of the tested extracts.</p> <p>Results</p> <p>A significant effect against the Gram positive and Gram negative reference bacterial strains was observed with all the extracts. The mutagenic and antimutagenic studies revealed that all the extracts decreased the mutagenicity induced by B(a)P (7.5 μg/plate), 2-AA (5 μg/plate), MMS (1.3 mg/plate) and NOPD (10 μg/plate). Likewise, all the extracts showed an important free radical scavenging activity towards the superoxide anion generated by the xanthine/xanthine oxidase assay system, as well as high Trolox Equivalent Antioxidant Capacity (TEAC), against the 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS)<sup>+</sup>• radical. TOF-enriched extract exhibited the highest protective effect against free radicals, direct acting-mutagen and metabolically activated S9-dependent mutagens.</p> <p>Conclusions</p> <p>The present study indicates that the extracts from <it>A. salicina </it>leaves are a significant source of compounds with the antimutagenic and antioxidant activities, and this may be useful for developing potential chemopreventive substances.</p> http://www.biomedcentral.com/1472-6882/12/37<it>Acacia salicina</it>Antigenotoxic activityAntioxidant activitiesAmes assay |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Boubaker Jihed Mansour Hedi Ghedira Kamel Ghedira Leila |
spellingShingle |
Boubaker Jihed Mansour Hedi Ghedira Kamel Ghedira Leila Polar extracts from (Tunisian) <it>Acacia salicina </it>Lindl. Study of the antimicrobial and antigenotoxic activities BMC Complementary and Alternative Medicine <it>Acacia salicina</it> Antigenotoxic activity Antioxidant activities Ames assay |
author_facet |
Boubaker Jihed Mansour Hedi Ghedira Kamel Ghedira Leila |
author_sort |
Boubaker Jihed |
title |
Polar extracts from (Tunisian) <it>Acacia salicina </it>Lindl. Study of the antimicrobial and antigenotoxic activities |
title_short |
Polar extracts from (Tunisian) <it>Acacia salicina </it>Lindl. Study of the antimicrobial and antigenotoxic activities |
title_full |
Polar extracts from (Tunisian) <it>Acacia salicina </it>Lindl. Study of the antimicrobial and antigenotoxic activities |
title_fullStr |
Polar extracts from (Tunisian) <it>Acacia salicina </it>Lindl. Study of the antimicrobial and antigenotoxic activities |
title_full_unstemmed |
Polar extracts from (Tunisian) <it>Acacia salicina </it>Lindl. Study of the antimicrobial and antigenotoxic activities |
title_sort |
polar extracts from (tunisian) <it>acacia salicina </it>lindl. study of the antimicrobial and antigenotoxic activities |
publisher |
BMC |
series |
BMC Complementary and Alternative Medicine |
issn |
1472-6882 |
publishDate |
2012-04-01 |
description |
<p>Abstract</p> <p>Background</p> <p>Methanolic, aqueous and Total Oligomer Flavonoids (TOF)-enriched extracts obtained from the leaves of <it>Acacia salicina </it>'Lindl.' were investigated for antibacterial, antimutagenic and antioxidant activities.</p> <p>Methods</p> <p>The antimicrobial activity was tested on the Gram positive and Gram negative reference bacterial strains. The Mutagenic and antimutagenic activities against direct acting mutagens, methylmethane sulfonate (MMS) and 4-nitro-o-phenylenediamine (NOPD), and indirect acting mutagens, 2-aminoanthracene (2-AA) and benzo[a]pyrene (B(a)P) were performed with <it>S. typhimurium </it>TA102 and TA98 assay systems. In addition, the enzymatic and nonenzymatic methods were employed to evaluate the anti-oxidative effects of the tested extracts.</p> <p>Results</p> <p>A significant effect against the Gram positive and Gram negative reference bacterial strains was observed with all the extracts. The mutagenic and antimutagenic studies revealed that all the extracts decreased the mutagenicity induced by B(a)P (7.5 μg/plate), 2-AA (5 μg/plate), MMS (1.3 mg/plate) and NOPD (10 μg/plate). Likewise, all the extracts showed an important free radical scavenging activity towards the superoxide anion generated by the xanthine/xanthine oxidase assay system, as well as high Trolox Equivalent Antioxidant Capacity (TEAC), against the 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS)<sup>+</sup>• radical. TOF-enriched extract exhibited the highest protective effect against free radicals, direct acting-mutagen and metabolically activated S9-dependent mutagens.</p> <p>Conclusions</p> <p>The present study indicates that the extracts from <it>A. salicina </it>leaves are a significant source of compounds with the antimutagenic and antioxidant activities, and this may be useful for developing potential chemopreventive substances.</p> |
topic |
<it>Acacia salicina</it> Antigenotoxic activity Antioxidant activities Ames assay |
url |
http://www.biomedcentral.com/1472-6882/12/37 |
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