Pitfalls in PCR troubleshooting: Expect the unexpected?
PCR is a well-understood and established laboratory technique often used in molecular diagnostics. Huge experience has been accumulated over the last years regarding the design of PCR assays and their set-up, including in-depth troubleshooting to obtain the optimal PCR assay for each purpose. Here w...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2016-01-01
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| Series: | Biomolecular Detection and Quantification |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214753515300097 |
| Summary: | PCR is a well-understood and established laboratory technique often used in molecular diagnostics. Huge experience has been accumulated over the last years regarding the design of PCR assays and their set-up, including in-depth troubleshooting to obtain the optimal PCR assay for each purpose. Here we report a PCR troubleshooting that came up with a surprising result never observed before. With this report we hope to sensitize the reader to this peculiar problem and to save troubleshooting efforts in similar situations, especially in time-critical and ambitious diagnostic settings. |
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| ISSN: | 2214-7535 |