Pitfalls in PCR troubleshooting: Expect the unexpected?

Pitfalls in PCR troubleshooting: Expect the unexpected?

PCR is a well-understood and established laboratory technique often used in molecular diagnostics. Huge experience has been accumulated over the last years regarding the design of PCR assays and their set-up, including in-depth troubleshooting to obtain the optimal PCR assay for each purpose. Here w...

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Bibliographic Details
Main Authors: Livia Schrick, Andreas Nitsche
Format: Article
Language:English
Published: Elsevier 2016-01-01
Series:Biomolecular Detection and Quantification
Subjects:
PCR
RT-PCR
Real-time PCR
PCR diagnostic
Troubleshooting
Reaction mix
Online Access:http://www.sciencedirect.com/science/article/pii/S2214753515300097
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spelling doaj-0c8d3c8447fc4cf093d29d4f89163f8e2020-11-25T00:09:21ZengElsevierBiomolecular Detection and Quantification2214-75352016-01-016C1310.1016/j.bdq.2015.11.001Pitfalls in PCR troubleshooting: Expect the unexpected?Livia SchrickAndreas NitschePCR is a well-understood and established laboratory technique often used in molecular diagnostics. Huge experience has been accumulated over the last years regarding the design of PCR assays and their set-up, including in-depth troubleshooting to obtain the optimal PCR assay for each purpose. Here we report a PCR troubleshooting that came up with a surprising result never observed before. With this report we hope to sensitize the reader to this peculiar problem and to save troubleshooting efforts in similar situations, especially in time-critical and ambitious diagnostic settings.http://www.sciencedirect.com/science/article/pii/S2214753515300097PCRRT-PCRReal-time PCRPCR diagnosticTroubleshootingReaction mix
collection DOAJ
language English
format Article
sources DOAJ
author Livia Schrick
Andreas Nitsche
spellingShingle Livia Schrick
Andreas Nitsche
Pitfalls in PCR troubleshooting: Expect the unexpected?
Biomolecular Detection and Quantification
PCR
RT-PCR
Real-time PCR
PCR diagnostic
Troubleshooting
Reaction mix
author_facet Livia Schrick
Andreas Nitsche
author_sort Livia Schrick
title Pitfalls in PCR troubleshooting: Expect the unexpected?
title_short Pitfalls in PCR troubleshooting: Expect the unexpected?
title_full Pitfalls in PCR troubleshooting: Expect the unexpected?
title_fullStr Pitfalls in PCR troubleshooting: Expect the unexpected?
title_full_unstemmed Pitfalls in PCR troubleshooting: Expect the unexpected?
title_sort pitfalls in pcr troubleshooting: expect the unexpected?
publisher Elsevier
series Biomolecular Detection and Quantification
issn 2214-7535
publishDate 2016-01-01
description PCR is a well-understood and established laboratory technique often used in molecular diagnostics. Huge experience has been accumulated over the last years regarding the design of PCR assays and their set-up, including in-depth troubleshooting to obtain the optimal PCR assay for each purpose. Here we report a PCR troubleshooting that came up with a surprising result never observed before. With this report we hope to sensitize the reader to this peculiar problem and to save troubleshooting efforts in similar situations, especially in time-critical and ambitious diagnostic settings.
topic PCR
RT-PCR
Real-time PCR
PCR diagnostic
Troubleshooting
Reaction mix
url http://www.sciencedirect.com/science/article/pii/S2214753515300097
work_keys_str_mv AT liviaschrick pitfallsinpcrtroubleshootingexpecttheunexpected
AT andreasnitsche pitfallsinpcrtroubleshootingexpecttheunexpected
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