The Arabidopsis V-ATPase is localized to the TGN/EE via a seed plant-specific motif
The V-ATPase is a versatile proton-pump found in a range of endomembrane compartments yet the mechanisms governing its differential targeting remain to be determined. In Arabidopsis, VHA-a1 targets the V-ATPase to the TGN/EE whereas VHA-a2 and VHA-a3 are localized to the tonoplast. We report here th...
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doaj-0c988ea6cc7447e9805c0d6c4c270ff52021-05-05T21:45:34ZengeLife Sciences Publications LtdeLife2050-084X2020-11-01910.7554/eLife.60568The Arabidopsis V-ATPase is localized to the TGN/EE via a seed plant-specific motifUpendo Lupanga0https://orcid.org/0000-0001-7732-5012Rachel Röhrich1Jana Askani2https://orcid.org/0000-0002-1390-7344Stefan Hilmer3Christiane Kiefer4Melanie Krebs5https://orcid.org/0000-0001-6858-3247Takehiko Kanazawa6Takashi Ueda7https://orcid.org/0000-0002-5190-892XKarin Schumacher8https://orcid.org/0000-0001-6484-8105Department of Cell Biology, Centre for Organismal Studies, Heidelberg University, Heidelberg, GermanyDepartment of Cell Biology, Centre for Organismal Studies, Heidelberg University, Heidelberg, GermanyDepartment of Cell Biology, Centre for Organismal Studies, Heidelberg University, Heidelberg, GermanyElectron Microscopy Core Facility, Heidelberg University, Heidelberg, GermanyDepartment of Biodiversity and Plant Systematics, Centre for Organismal Studies, Heidelberg University, Heidelberg, GermanyDepartment of Cell Biology, Centre for Organismal Studies, Heidelberg University, Heidelberg, GermanyDivision of Cellular Dynamics, National Institute for Basic Biology, OkazakiAichi, Japan; The Department of Basic Biology, SOKENDAI (The Graduate University for Advanced Studies), OkazakiAichi, JapanDivision of Cellular Dynamics, National Institute for Basic Biology, OkazakiAichi, Japan; The Department of Basic Biology, SOKENDAI (The Graduate University for Advanced Studies), OkazakiAichi, JapanDepartment of Cell Biology, Centre for Organismal Studies, Heidelberg University, Heidelberg, GermanyThe V-ATPase is a versatile proton-pump found in a range of endomembrane compartments yet the mechanisms governing its differential targeting remain to be determined. In Arabidopsis, VHA-a1 targets the V-ATPase to the TGN/EE whereas VHA-a2 and VHA-a3 are localized to the tonoplast. We report here that the VHA-a1 targeting domain serves as both an ER-exit and as a TGN/EE-retention motif and is conserved among seed plants. In contrast, Marchantia encodes a single VHA-isoform that localizes to the TGN/EE and the tonoplast in Arabidopsis. Analysis of CRISPR/Cas9 generated null alleles revealed that VHA-a1 has an essential function for male gametophyte development but acts redundantly with the tonoplast isoforms during vegetative growth. We propose that in the absence of VHA-a1, VHA-a3 is partially re-routed to the TGN/EE. Our findings contribute to understanding the evolutionary origin of V-ATPase targeting and provide a striking example that differential localization does not preclude functional redundancy.https://elifesciences.org/articles/60568V-ATPasetargetingTGN/EEMarchantia polymorphaproton pumpCRISPR |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Upendo Lupanga Rachel Röhrich Jana Askani Stefan Hilmer Christiane Kiefer Melanie Krebs Takehiko Kanazawa Takashi Ueda Karin Schumacher |
spellingShingle |
Upendo Lupanga Rachel Röhrich Jana Askani Stefan Hilmer Christiane Kiefer Melanie Krebs Takehiko Kanazawa Takashi Ueda Karin Schumacher The Arabidopsis V-ATPase is localized to the TGN/EE via a seed plant-specific motif eLife V-ATPase targeting TGN/EE Marchantia polymorpha proton pump CRISPR |
author_facet |
Upendo Lupanga Rachel Röhrich Jana Askani Stefan Hilmer Christiane Kiefer Melanie Krebs Takehiko Kanazawa Takashi Ueda Karin Schumacher |
author_sort |
Upendo Lupanga |
title |
The Arabidopsis V-ATPase is localized to the TGN/EE via a seed plant-specific motif |
title_short |
The Arabidopsis V-ATPase is localized to the TGN/EE via a seed plant-specific motif |
title_full |
The Arabidopsis V-ATPase is localized to the TGN/EE via a seed plant-specific motif |
title_fullStr |
The Arabidopsis V-ATPase is localized to the TGN/EE via a seed plant-specific motif |
title_full_unstemmed |
The Arabidopsis V-ATPase is localized to the TGN/EE via a seed plant-specific motif |
title_sort |
arabidopsis v-atpase is localized to the tgn/ee via a seed plant-specific motif |
publisher |
eLife Sciences Publications Ltd |
series |
eLife |
issn |
2050-084X |
publishDate |
2020-11-01 |
description |
The V-ATPase is a versatile proton-pump found in a range of endomembrane compartments yet the mechanisms governing its differential targeting remain to be determined. In Arabidopsis, VHA-a1 targets the V-ATPase to the TGN/EE whereas VHA-a2 and VHA-a3 are localized to the tonoplast. We report here that the VHA-a1 targeting domain serves as both an ER-exit and as a TGN/EE-retention motif and is conserved among seed plants. In contrast, Marchantia encodes a single VHA-isoform that localizes to the TGN/EE and the tonoplast in Arabidopsis. Analysis of CRISPR/Cas9 generated null alleles revealed that VHA-a1 has an essential function for male gametophyte development but acts redundantly with the tonoplast isoforms during vegetative growth. We propose that in the absence of VHA-a1, VHA-a3 is partially re-routed to the TGN/EE. Our findings contribute to understanding the evolutionary origin of V-ATPase targeting and provide a striking example that differential localization does not preclude functional redundancy. |
topic |
V-ATPase targeting TGN/EE Marchantia polymorpha proton pump CRISPR |
url |
https://elifesciences.org/articles/60568 |
work_keys_str_mv |
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