Oleaginous yeasts respond differently to carbon sources present in lignocellulose hydrolysate
Abstract Background Microbial oils, generated from lignocellulosic material, have great potential as renewable and sustainable alternatives to fossil-based fuels and chemicals. By unravelling the diversity of lipid accumulation physiology in different oleaginous yeasts grown on the various carbon so...
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doaj-0cb5c89185534da9bef4503e047496e22021-05-30T11:48:36ZengBMCBiotechnology for Biofuels1754-68342021-05-0114111210.1186/s13068-021-01974-2Oleaginous yeasts respond differently to carbon sources present in lignocellulose hydrolysateJule Brandenburg0Johanna Blomqvist1Volha Shapaval2Achim Kohler3Sabine Sampels4Mats Sandgren5Volkmar Passoth6Department of Molecular Sciences, Swedish University of Agricultural Sciences, BioCenterDepartment of Molecular Sciences, Swedish University of Agricultural Sciences, BioCenterFaculty of Science and Technology, Norwegian University of Life SciencesFaculty of Science and Technology, Norwegian University of Life SciencesDepartment of Molecular Sciences, Swedish University of Agricultural Sciences, BioCenterDepartment of Molecular Sciences, Swedish University of Agricultural Sciences, BioCenterDepartment of Molecular Sciences, Swedish University of Agricultural Sciences, BioCenterAbstract Background Microbial oils, generated from lignocellulosic material, have great potential as renewable and sustainable alternatives to fossil-based fuels and chemicals. By unravelling the diversity of lipid accumulation physiology in different oleaginous yeasts grown on the various carbon sources present in lignocellulose hydrolysate (LH), new targets for optimisation of lipid accumulation can be identified. Monitoring lipid formation over time is essential for understanding lipid accumulation physiology. This study investigated lipid accumulation in a variety of oleaginous ascomycetous and basidiomycetous strains grown in glucose and xylose and followed lipid formation kinetics of selected strains in wheat straw hydrolysate (WSH). Results Twenty-nine oleaginous yeast strains were tested for their ability to utilise glucose and xylose, the main sugars present in WSH. Evaluation of sugar consumption and lipid accumulation revealed marked differences in xylose utilisation capacity between the yeast strains, even between those belonging to the same species. Five different promising strains, belonging to the species Lipomyces starkeyi, Rhodotorula glutinis, Rhodotorula babjevae and Rhodotorula toruloides, were grown on undiluted wheat straw hydrolysate and lipid accumulation was followed over time, using Fourier transform-infrared (FTIR) spectroscopy. All five strains were able to grow on undiluted WSH and to accumulate lipids, but to different extents and with different productivities. R. babjevae DVBPG 8058 was the best-performing strain, accumulating 64.8% of cell dry weight (CDW) as lipids. It reached a culture density of 28 g/L CDW in batch cultivation, resulting in a lipid content of 18.1 g/L and yield of 0.24 g lipids per g carbon source. This strain formed lipids from the major carbon sources in hydrolysate, glucose, acetate and xylose. R. glutinis CBS 2367 also consumed these carbon sources, but when assimilating xylose it consumed intracellular lipids simultaneously. Rhodotorula strains contained a higher proportion of polyunsaturated fatty acids than the two tested Lipomyces starkeyi strains. Conclusions There is considerable metabolic diversity among oleaginous yeasts, even between closely related species and strains, especially when converting xylose to biomass and lipids. Monitoring the kinetics of lipid accumulation and identifying the molecular basis of this diversity are keys to selecting suitable strains for high lipid production from lignocellulose.https://doi.org/10.1186/s13068-021-01974-2Oleaginous yeastsAscomycetesBasidiomycetesFTIRLipidsLignocellulose |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jule Brandenburg Johanna Blomqvist Volha Shapaval Achim Kohler Sabine Sampels Mats Sandgren Volkmar Passoth |
spellingShingle |
Jule Brandenburg Johanna Blomqvist Volha Shapaval Achim Kohler Sabine Sampels Mats Sandgren Volkmar Passoth Oleaginous yeasts respond differently to carbon sources present in lignocellulose hydrolysate Biotechnology for Biofuels Oleaginous yeasts Ascomycetes Basidiomycetes FTIR Lipids Lignocellulose |
author_facet |
Jule Brandenburg Johanna Blomqvist Volha Shapaval Achim Kohler Sabine Sampels Mats Sandgren Volkmar Passoth |
author_sort |
Jule Brandenburg |
title |
Oleaginous yeasts respond differently to carbon sources present in lignocellulose hydrolysate |
title_short |
Oleaginous yeasts respond differently to carbon sources present in lignocellulose hydrolysate |
title_full |
Oleaginous yeasts respond differently to carbon sources present in lignocellulose hydrolysate |
title_fullStr |
Oleaginous yeasts respond differently to carbon sources present in lignocellulose hydrolysate |
title_full_unstemmed |
Oleaginous yeasts respond differently to carbon sources present in lignocellulose hydrolysate |
title_sort |
oleaginous yeasts respond differently to carbon sources present in lignocellulose hydrolysate |
publisher |
BMC |
series |
Biotechnology for Biofuels |
issn |
1754-6834 |
publishDate |
2021-05-01 |
description |
Abstract Background Microbial oils, generated from lignocellulosic material, have great potential as renewable and sustainable alternatives to fossil-based fuels and chemicals. By unravelling the diversity of lipid accumulation physiology in different oleaginous yeasts grown on the various carbon sources present in lignocellulose hydrolysate (LH), new targets for optimisation of lipid accumulation can be identified. Monitoring lipid formation over time is essential for understanding lipid accumulation physiology. This study investigated lipid accumulation in a variety of oleaginous ascomycetous and basidiomycetous strains grown in glucose and xylose and followed lipid formation kinetics of selected strains in wheat straw hydrolysate (WSH). Results Twenty-nine oleaginous yeast strains were tested for their ability to utilise glucose and xylose, the main sugars present in WSH. Evaluation of sugar consumption and lipid accumulation revealed marked differences in xylose utilisation capacity between the yeast strains, even between those belonging to the same species. Five different promising strains, belonging to the species Lipomyces starkeyi, Rhodotorula glutinis, Rhodotorula babjevae and Rhodotorula toruloides, were grown on undiluted wheat straw hydrolysate and lipid accumulation was followed over time, using Fourier transform-infrared (FTIR) spectroscopy. All five strains were able to grow on undiluted WSH and to accumulate lipids, but to different extents and with different productivities. R. babjevae DVBPG 8058 was the best-performing strain, accumulating 64.8% of cell dry weight (CDW) as lipids. It reached a culture density of 28 g/L CDW in batch cultivation, resulting in a lipid content of 18.1 g/L and yield of 0.24 g lipids per g carbon source. This strain formed lipids from the major carbon sources in hydrolysate, glucose, acetate and xylose. R. glutinis CBS 2367 also consumed these carbon sources, but when assimilating xylose it consumed intracellular lipids simultaneously. Rhodotorula strains contained a higher proportion of polyunsaturated fatty acids than the two tested Lipomyces starkeyi strains. Conclusions There is considerable metabolic diversity among oleaginous yeasts, even between closely related species and strains, especially when converting xylose to biomass and lipids. Monitoring the kinetics of lipid accumulation and identifying the molecular basis of this diversity are keys to selecting suitable strains for high lipid production from lignocellulose. |
topic |
Oleaginous yeasts Ascomycetes Basidiomycetes FTIR Lipids Lignocellulose |
url |
https://doi.org/10.1186/s13068-021-01974-2 |
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