In vitro cytotoxicity and anti-inflammatory cytokinine activity study of three isolated novel compounds of Prismatomeris glabra

• Main Authors: Khaled A A Alkadi, Kamran Ashraf, Aishah Adam, Syed Adnan Ali Shah, Muhammad Taha, Mizaton Hazizul Hasan, Cinimathew John, Razali Mohamed Salleh, Wasim Ahmad
• Format: Article
• Language: English
• Published: Wolters Kluwer Medknow Publications 2021-01-01
• Series: Journal of Pharmacy and Bioallied Sciences
• Subjects: amentoflavone; anti-inflammatory; cytotoxicity; prismatomeris glabra
• Online Access: http://www.jpbsonline.org/article.asp?issn=0975-7406;year=2021;volume=13;issue=1;spage=116;epage=122;aulast=Alkadi

Objectives: The aim of the present study was to isolate and evaluate cytotoxicity and anti-inflammatory activities of new novel compounds isolated from Prismatomeris glabra. Materials and Methods: Dried root of P. glabra was extracted under reflux with methyl alcohol, fractionated through the vacuum liquid chromatography technique, and evaporated and then purified the compounds using column chromatography and preparative thin-layer chromatography. THP-1 cells were treated with amentoflavone, 5,7,4′-hydroxyflavonoid, and stigmasterol with various concentrations (0–30 µg/mL) and then incubated with MTS reagent for 2h. Treatment was done for 24, 48, and 72h. Then, effects of these compounds were also tested on PGE2, TNF-α, and IL-6 expression in human THP-1-derived macrophage cells for 24h. Results: Three new compounds such as amentoflavone, 5,7,4′-hydroxyflavonoid, and stigmasterol were isolated. After 24h of incubation, a significant decrease in cell viability was reported with IC50 values of amentoflavone, 5,7,4′- hydroxyflavonoid, and stigmasterol (21 µg/mL ≡ 38 M), (18 µg/mL ≡ 66 M) and (20 µg/mL ≡ 48.5 M), respectively. Whereas for 48 and 72h treatment showed a less decreased cell viability compared with 24h treatment. These compounds also showed a significant reduction in the production of TNF-α, IL-6, and PGE2 in a dose-dependent manner. Conclusions: The isolated new compounds showed significant cytotoxicity and anti-inflammatory effects.