Extraneous E-Cadherin Engages the Deterministic Process of Somatic Reprogramming through Modulating STAT3 and Erk1/2 Activity
Although several modes of reprogramming have been reported in different cell types during iPSC induction, the molecular mechanism regarding the selection of different modes of action is still mostly unknown. The present study examined the molecular events that participate in the selection of such pr...
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doaj-0cf14ebd65bd471aba8f1c74dee9b61d2021-02-01T00:00:56ZengMDPI AGCells2073-44092021-01-011028428410.3390/cells10020284Extraneous E-Cadherin Engages the Deterministic Process of Somatic Reprogramming through Modulating STAT3 and Erk1/2 ActivityYu-Hao Liu0Chien-Chang Chen1Yi-Jen Hsueh2Li-Man Hung3David Hui-Kang Ma4Hung-Chi Chen5Wen-Bin Len6Yaa-Jyuhn J. Meir7Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan 33302, TaiwanGraduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan 33302, TaiwanCenter for Tissue Engineering, Chang Gung Memorial Hospital, Linkou 33305, TaiwanGraduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan 33302, TaiwanCenter for Tissue Engineering, Chang Gung Memorial Hospital, Linkou 33305, TaiwanCenter for Tissue Engineering, Chang Gung Memorial Hospital, Linkou 33305, TaiwanDepartment of Nursing, Chang Gung University of Science and Technology, Taoyuan 33302, TaiwanGraduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan 33302, TaiwanAlthough several modes of reprogramming have been reported in different cell types during iPSC induction, the molecular mechanism regarding the selection of different modes of action is still mostly unknown. The present study examined the molecular events that participate in the selection of such processes at the onset of somatic reprogramming. The activity of STAT3 versus that of Erk1/2 reversibly determines the reprogramming mode entered; a lower activity ratio favors the deterministic process and vice versa. Additionally, extraneous E-cadherin facilitates the early events of somatic reprogramming, potentially by stabilizing the LIF/gp130 and EGFR/ErbB2 complexes to promote entry into the deterministic process. Our current findings demonstrated that manipulating the pSTAT3/pErk1/2 activity ratio in the surrounding milieu can drive different modes of action toward either the deterministic or the stochastic process in the context of OSKM-mediated somatic reprogramming.https://www.mdpi.com/2073-4409/10/2/284induced pluripotent stem cell (iPSC)somatic reprogrammingCol1a1 4F2A Oct4-GFPreprogrammable mousestochastic and deterministic model |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yu-Hao Liu Chien-Chang Chen Yi-Jen Hsueh Li-Man Hung David Hui-Kang Ma Hung-Chi Chen Wen-Bin Len Yaa-Jyuhn J. Meir |
spellingShingle |
Yu-Hao Liu Chien-Chang Chen Yi-Jen Hsueh Li-Man Hung David Hui-Kang Ma Hung-Chi Chen Wen-Bin Len Yaa-Jyuhn J. Meir Extraneous E-Cadherin Engages the Deterministic Process of Somatic Reprogramming through Modulating STAT3 and Erk1/2 Activity Cells induced pluripotent stem cell (iPSC) somatic reprogramming Col1a1 4F2A Oct4-GFP reprogrammable mouse stochastic and deterministic model |
author_facet |
Yu-Hao Liu Chien-Chang Chen Yi-Jen Hsueh Li-Man Hung David Hui-Kang Ma Hung-Chi Chen Wen-Bin Len Yaa-Jyuhn J. Meir |
author_sort |
Yu-Hao Liu |
title |
Extraneous E-Cadherin Engages the Deterministic Process of Somatic Reprogramming through Modulating STAT3 and Erk1/2 Activity |
title_short |
Extraneous E-Cadherin Engages the Deterministic Process of Somatic Reprogramming through Modulating STAT3 and Erk1/2 Activity |
title_full |
Extraneous E-Cadherin Engages the Deterministic Process of Somatic Reprogramming through Modulating STAT3 and Erk1/2 Activity |
title_fullStr |
Extraneous E-Cadherin Engages the Deterministic Process of Somatic Reprogramming through Modulating STAT3 and Erk1/2 Activity |
title_full_unstemmed |
Extraneous E-Cadherin Engages the Deterministic Process of Somatic Reprogramming through Modulating STAT3 and Erk1/2 Activity |
title_sort |
extraneous e-cadherin engages the deterministic process of somatic reprogramming through modulating stat3 and erk1/2 activity |
publisher |
MDPI AG |
series |
Cells |
issn |
2073-4409 |
publishDate |
2021-01-01 |
description |
Although several modes of reprogramming have been reported in different cell types during iPSC induction, the molecular mechanism regarding the selection of different modes of action is still mostly unknown. The present study examined the molecular events that participate in the selection of such processes at the onset of somatic reprogramming. The activity of STAT3 versus that of Erk1/2 reversibly determines the reprogramming mode entered; a lower activity ratio favors the deterministic process and vice versa. Additionally, extraneous E-cadherin facilitates the early events of somatic reprogramming, potentially by stabilizing the LIF/gp130 and EGFR/ErbB2 complexes to promote entry into the deterministic process. Our current findings demonstrated that manipulating the pSTAT3/pErk1/2 activity ratio in the surrounding milieu can drive different modes of action toward either the deterministic or the stochastic process in the context of OSKM-mediated somatic reprogramming. |
topic |
induced pluripotent stem cell (iPSC) somatic reprogramming Col1a1 4F2A Oct4-GFP reprogrammable mouse stochastic and deterministic model |
url |
https://www.mdpi.com/2073-4409/10/2/284 |
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