Context-Dependent Regulation of Conjunctival Goblet Cell Function by Allergic Mediators
Abstract In the eye, goblet cells responsible for secreting mucins are found in the conjunctiva. When mucin production is not tightly regulated several ocular surface disorders may occur. In this study, the effect of the T helper (Th) 2-type cytokines IL4, IL5, and IL13 on conjunctival goblet cell f...
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2018-08-01
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Online Access: | https://doi.org/10.1038/s41598-018-30002-x |
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doaj-0cf80d37144944938217eb5a2176f34d2020-12-08T04:04:37ZengNature Publishing GroupScientific Reports2045-23222018-08-018111110.1038/s41598-018-30002-xContext-Dependent Regulation of Conjunctival Goblet Cell Function by Allergic MediatorsLaura García-Posadas0Robin R. Hodges1Yolanda Diebold2Darlene A. Dartt3Schepens Eye Research Institute/Massachusetts Eye and Ear, Harvard Medical SchoolSchepens Eye Research Institute/Massachusetts Eye and Ear, Harvard Medical SchoolOcular Surface Group, Institute for Applied Ophthalmobiology (IOBA), University of ValladolidSchepens Eye Research Institute/Massachusetts Eye and Ear, Harvard Medical SchoolAbstract In the eye, goblet cells responsible for secreting mucins are found in the conjunctiva. When mucin production is not tightly regulated several ocular surface disorders may occur. In this study, the effect of the T helper (Th) 2-type cytokines IL4, IL5, and IL13 on conjunctival goblet cell function was explored. Goblet cells from rat conjunctiva were cultured and characterized. The presence of cytokine receptors was confirmed by Reverse Transcription-Polymerase Chain Reaction (RT-PCR). Changes in intracellular [Ca2+], high molecular weight glycoconjugate secretion, and proliferation were measured after stimulation with Th2 cytokines with or without the allergic mediator histamine. We found that IL4 and IL13 enhance cell proliferation and, along with histamine, stimulate goblet cell secretion. We conclude that the high levels of IL4, IL5, and IL13 that characterize allergic conjunctivitis could be the reason for higher numbers of goblet cells and mucin overproduction found in this condition.https://doi.org/10.1038/s41598-018-30002-x |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Laura García-Posadas Robin R. Hodges Yolanda Diebold Darlene A. Dartt |
spellingShingle |
Laura García-Posadas Robin R. Hodges Yolanda Diebold Darlene A. Dartt Context-Dependent Regulation of Conjunctival Goblet Cell Function by Allergic Mediators Scientific Reports |
author_facet |
Laura García-Posadas Robin R. Hodges Yolanda Diebold Darlene A. Dartt |
author_sort |
Laura García-Posadas |
title |
Context-Dependent Regulation of Conjunctival Goblet Cell Function by Allergic Mediators |
title_short |
Context-Dependent Regulation of Conjunctival Goblet Cell Function by Allergic Mediators |
title_full |
Context-Dependent Regulation of Conjunctival Goblet Cell Function by Allergic Mediators |
title_fullStr |
Context-Dependent Regulation of Conjunctival Goblet Cell Function by Allergic Mediators |
title_full_unstemmed |
Context-Dependent Regulation of Conjunctival Goblet Cell Function by Allergic Mediators |
title_sort |
context-dependent regulation of conjunctival goblet cell function by allergic mediators |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2018-08-01 |
description |
Abstract In the eye, goblet cells responsible for secreting mucins are found in the conjunctiva. When mucin production is not tightly regulated several ocular surface disorders may occur. In this study, the effect of the T helper (Th) 2-type cytokines IL4, IL5, and IL13 on conjunctival goblet cell function was explored. Goblet cells from rat conjunctiva were cultured and characterized. The presence of cytokine receptors was confirmed by Reverse Transcription-Polymerase Chain Reaction (RT-PCR). Changes in intracellular [Ca2+], high molecular weight glycoconjugate secretion, and proliferation were measured after stimulation with Th2 cytokines with or without the allergic mediator histamine. We found that IL4 and IL13 enhance cell proliferation and, along with histamine, stimulate goblet cell secretion. We conclude that the high levels of IL4, IL5, and IL13 that characterize allergic conjunctivitis could be the reason for higher numbers of goblet cells and mucin overproduction found in this condition. |
url |
https://doi.org/10.1038/s41598-018-30002-x |
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