Automated cleaning and pre-processing of immunoglobulin gene sequences from high-throughput sequencing
High throughput sequencing (HTS) yields tens of thousands to millions of sequences that require a large amount of pre-processing work to clean various artifacts. Such cleaning cannot be performed manually. Existing programs are not suitable for immunoglobulin (Ig) genes, which are variable and often...
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2012-12-01
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doaj-0cff8df78d9d4eb0b72c1281ba7ea6222020-11-24T21:42:14ZengFrontiers Media S.A.Frontiers in Immunology1664-32242012-12-01310.3389/fimmu.2012.0038633663Automated cleaning and pre-processing of immunoglobulin gene sequences from high-throughput sequencingMiri eMichaeli0Hila eNoga1Hilla eTabibian-Keissar2Hilla eTabibian-Keissar3Iris eBarshack4Iris eBarshack5Ramit eMehr6Bar-Ilan UniversityBar-Ilan UniversityBar-Ilan UniversitySheba Medical CenterSheba Medical CenterTel Aviv UniversityBar-Ilan UniversityHigh throughput sequencing (HTS) yields tens of thousands to millions of sequences that require a large amount of pre-processing work to clean various artifacts. Such cleaning cannot be performed manually. Existing programs are not suitable for immunoglobulin (Ig) genes, which are variable and often highly mutated. This paper describes Ig-HTS-Cleaner (Ig High Throughput Sequencing Cleaner), a program containing a simple cleaning procedure that successfully deals with pre-processing of Ig sequences derived from HTS, and Ig-Indel-Identifier (Ig Insertion – Deletion Identifier), a program for identifying legitimate and artifact insertions and/or deletions (indels). Our programs were designed for analyzing Ig gene sequences obtained by 454 sequencing, but they are applicable to all types of sequences and sequencing platforms. Ig-HTS-Cleaner and Ig-Indel-Identifier have been implemented in Java and saved as executable JAR files, supported on Linux and MS Windows. No special requirements are needed in order to run the programs, except for correctly constructing the input files as explained in the text. The programs' performance has been tested and validated on real and simulated data sets.http://journal.frontiersin.org/Journal/10.3389/fimmu.2012.00386/fullhigh-throughput sequencingB cell receptorComputer programsImmunoglobulin (Ig) genesinsertions and deletions (indels). |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Miri eMichaeli Hila eNoga Hilla eTabibian-Keissar Hilla eTabibian-Keissar Iris eBarshack Iris eBarshack Ramit eMehr |
spellingShingle |
Miri eMichaeli Hila eNoga Hilla eTabibian-Keissar Hilla eTabibian-Keissar Iris eBarshack Iris eBarshack Ramit eMehr Automated cleaning and pre-processing of immunoglobulin gene sequences from high-throughput sequencing Frontiers in Immunology high-throughput sequencing B cell receptor Computer programs Immunoglobulin (Ig) genes insertions and deletions (indels). |
author_facet |
Miri eMichaeli Hila eNoga Hilla eTabibian-Keissar Hilla eTabibian-Keissar Iris eBarshack Iris eBarshack Ramit eMehr |
author_sort |
Miri eMichaeli |
title |
Automated cleaning and pre-processing of immunoglobulin gene sequences from high-throughput sequencing |
title_short |
Automated cleaning and pre-processing of immunoglobulin gene sequences from high-throughput sequencing |
title_full |
Automated cleaning and pre-processing of immunoglobulin gene sequences from high-throughput sequencing |
title_fullStr |
Automated cleaning and pre-processing of immunoglobulin gene sequences from high-throughput sequencing |
title_full_unstemmed |
Automated cleaning and pre-processing of immunoglobulin gene sequences from high-throughput sequencing |
title_sort |
automated cleaning and pre-processing of immunoglobulin gene sequences from high-throughput sequencing |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Immunology |
issn |
1664-3224 |
publishDate |
2012-12-01 |
description |
High throughput sequencing (HTS) yields tens of thousands to millions of sequences that require a large amount of pre-processing work to clean various artifacts. Such cleaning cannot be performed manually. Existing programs are not suitable for immunoglobulin (Ig) genes, which are variable and often highly mutated. This paper describes Ig-HTS-Cleaner (Ig High Throughput Sequencing Cleaner), a program containing a simple cleaning procedure that successfully deals with pre-processing of Ig sequences derived from HTS, and Ig-Indel-Identifier (Ig Insertion – Deletion Identifier), a program for identifying legitimate and artifact insertions and/or deletions (indels). Our programs were designed for analyzing Ig gene sequences obtained by 454 sequencing, but they are applicable to all types of sequences and sequencing platforms. Ig-HTS-Cleaner and Ig-Indel-Identifier have been implemented in Java and saved as executable JAR files, supported on Linux and MS Windows. No special requirements are needed in order to run the programs, except for correctly constructing the input files as explained in the text. The programs' performance has been tested and validated on real and simulated data sets. |
topic |
high-throughput sequencing B cell receptor Computer programs Immunoglobulin (Ig) genes insertions and deletions (indels). |
url |
http://journal.frontiersin.org/Journal/10.3389/fimmu.2012.00386/full |
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