An Apta-Biosensor for Colon Cancer Diagnostics

• Main Authors: Mojgan Ahmadzadeh Raji, Ghasem Amoabediny, Parviz Tajik, Morteza Hosseini, Ebrahim Ghafar-Zadeh
• Format: Article
• Language: English
• Published: MDPI AG 2015-09-01
• Series: Sensors
• Subjects: aptasensor; aptamer; cyclic voltammetry (CV); colon cancer; biosensor; fluorescence labeling
• Online Access: http://www.mdpi.com/1424-8220/15/9/22291

This paper reports the design and implementation of an aptasensor using a modified KCHA10a aptamer. This aptasensor consists of a functionalized electrodes using various materials including 11-mercaptoandecanoic acid (11-MUA) and modified KCHA10a aptamer. The HCT 116, HT 29 and HEp-2 cell lines are used in this study to demonstrate the functionality of aptasensor for colon cancer detection purposes. Flow cytometry, fluorescence microscopy and electrochemical cyclic voltammetry are used to verify the binding between the target cells and aptamer. The limit of detection (LOD) of this aptasensor is equal to seven cancer cells. Based on the experimental results, the proposed sensor can be employed for point-of-care cancer disease diagnostics.