Immunomodulation and Intestinal Morpho-Functional Aspects of a Novel Gram-Negative Bacterium Rouxiella badensis subsp. acadiensis
A novel bacterium (Rouxiella badensis subsp. acadiensis) isolated from the microbiota of wild blueberry fruit was investigated for its immunomodulation capabilities and intestinal morpho-functional aspects. The whole-genome shotgun sequencing of this bacterium led to its new taxonomy and showed abse...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2021-06-01
|
Series: | Frontiers in Microbiology |
Subjects: | |
Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.569119/full |
id |
doaj-0d1419b11c1044b98888115199d2af0f |
---|---|
record_format |
Article |
spelling |
doaj-0d1419b11c1044b98888115199d2af0f2021-06-22T06:40:47ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-06-011210.3389/fmicb.2021.569119569119Immunomodulation and Intestinal Morpho-Functional Aspects of a Novel Gram-Negative Bacterium Rouxiella badensis subsp. acadiensisNour Yahfoufi0Nawal Alsadi1Jean Francois Mallet2Garima Kulshreshtha3Maxwell Hincke4Maxwell Hincke5Nafissa Ismail6Chantal Matar7Chantal Matar8Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, Ottawa, ON, CanadaDepartment of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, Ottawa, ON, CanadaDepartment of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, Ottawa, ON, CanadaDepartment of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, Ottawa, ON, CanadaDepartment of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, Ottawa, ON, CanadaDepartment of Innovation in Medical education, Faculty of Medicine, University of Ottawa, Ottawa, ON, CanadaSchool of Psychology, Faculty of Social Sciences, University of Ottawa, Ottawa, ON, CanadaDepartment of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, Ottawa, ON, CanadaSchool of Nutrition, Faculty of Health Sciences, University of Ottawa, Ottawa, ON, CanadaA novel bacterium (Rouxiella badensis subsp. acadiensis) isolated from the microbiota of wild blueberry fruit was investigated for its immunomodulation capabilities and intestinal morpho-functional aspects. The whole-genome shotgun sequencing of this bacterium led to its new taxonomy and showed absence of pathogenicity genes. Although the bacterium was used for blueberry-fermentation and enhancing its anti-inflammatory effects on neurodegeneration, diabetes, and cancer, no study has assessed the effect of the bacterium on health. In this study, we used several in vitro and in vivo assays to evaluate the interaction of R. badensis subsp. acadiensis with the intestinal mucosa and its impact on the localized immune response. The strain antibiotic susceptibility has been investigated as well as its tolerance to gastric and intestinal environment and ability to attach to human intestinal epithelial cells (Caco-2 and HT-29). In addition, Balb/c mice were used to explore the immune-modulatory characteristics of the live bacterium at the intestinal level and its impact on the morpho-functional aspects of the intestinal mucosa. In vitro assays revealed the ability of R. badensis subsp. acadiensis to survive the gastric and intestinal simulated conditions and to satisfactorily adhere to the human intestinal epithelial cells. The bacterium was shown to be sensitive to an array of antibiotics. Immuno-modulation studies with mice orally administered with R. badensis subsp. acadiensis showed a higher number of IgA positive cells in the small intestine, a higher concentration of the anti-inflammatory cytokine IL-10 in the intestinal mucosa, as well as an increase in the number of goblet cells. The anti-inflammatory cytokine miR146a was found to be increased in the ileum and brain. Furthermore, it increases the number of goblet cells which contribute to intestinal barrier integrity. Taken together, our findings reflect the ability of the tested bacterium to modulates the intestinal homeostasis and immune response. Detailed safety unpublished studies and genome data support our finding. The strain Rouxiella badensis subsp. acadiensis has been filed in a provisional patent; a U.S. Provisional Application No. 62/916,921 entitled “Probiotics Composition and Methods.” Future studies are still needed to validate the potential utilization of this strain as functional food and its potential probiotic effect.https://www.frontiersin.org/articles/10.3389/fmicb.2021.569119/fullprobioticmucosal immunityimmuno-modulationmicoRNAs (miRNAs)gastro-intestinal tolerance |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Nour Yahfoufi Nawal Alsadi Jean Francois Mallet Garima Kulshreshtha Maxwell Hincke Maxwell Hincke Nafissa Ismail Chantal Matar Chantal Matar |
spellingShingle |
Nour Yahfoufi Nawal Alsadi Jean Francois Mallet Garima Kulshreshtha Maxwell Hincke Maxwell Hincke Nafissa Ismail Chantal Matar Chantal Matar Immunomodulation and Intestinal Morpho-Functional Aspects of a Novel Gram-Negative Bacterium Rouxiella badensis subsp. acadiensis Frontiers in Microbiology probiotic mucosal immunity immuno-modulation micoRNAs (miRNAs) gastro-intestinal tolerance |
author_facet |
Nour Yahfoufi Nawal Alsadi Jean Francois Mallet Garima Kulshreshtha Maxwell Hincke Maxwell Hincke Nafissa Ismail Chantal Matar Chantal Matar |
author_sort |
Nour Yahfoufi |
title |
Immunomodulation and Intestinal Morpho-Functional Aspects of a Novel Gram-Negative Bacterium Rouxiella badensis subsp. acadiensis |
title_short |
Immunomodulation and Intestinal Morpho-Functional Aspects of a Novel Gram-Negative Bacterium Rouxiella badensis subsp. acadiensis |
title_full |
Immunomodulation and Intestinal Morpho-Functional Aspects of a Novel Gram-Negative Bacterium Rouxiella badensis subsp. acadiensis |
title_fullStr |
Immunomodulation and Intestinal Morpho-Functional Aspects of a Novel Gram-Negative Bacterium Rouxiella badensis subsp. acadiensis |
title_full_unstemmed |
Immunomodulation and Intestinal Morpho-Functional Aspects of a Novel Gram-Negative Bacterium Rouxiella badensis subsp. acadiensis |
title_sort |
immunomodulation and intestinal morpho-functional aspects of a novel gram-negative bacterium rouxiella badensis subsp. acadiensis |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2021-06-01 |
description |
A novel bacterium (Rouxiella badensis subsp. acadiensis) isolated from the microbiota of wild blueberry fruit was investigated for its immunomodulation capabilities and intestinal morpho-functional aspects. The whole-genome shotgun sequencing of this bacterium led to its new taxonomy and showed absence of pathogenicity genes. Although the bacterium was used for blueberry-fermentation and enhancing its anti-inflammatory effects on neurodegeneration, diabetes, and cancer, no study has assessed the effect of the bacterium on health. In this study, we used several in vitro and in vivo assays to evaluate the interaction of R. badensis subsp. acadiensis with the intestinal mucosa and its impact on the localized immune response. The strain antibiotic susceptibility has been investigated as well as its tolerance to gastric and intestinal environment and ability to attach to human intestinal epithelial cells (Caco-2 and HT-29). In addition, Balb/c mice were used to explore the immune-modulatory characteristics of the live bacterium at the intestinal level and its impact on the morpho-functional aspects of the intestinal mucosa. In vitro assays revealed the ability of R. badensis subsp. acadiensis to survive the gastric and intestinal simulated conditions and to satisfactorily adhere to the human intestinal epithelial cells. The bacterium was shown to be sensitive to an array of antibiotics. Immuno-modulation studies with mice orally administered with R. badensis subsp. acadiensis showed a higher number of IgA positive cells in the small intestine, a higher concentration of the anti-inflammatory cytokine IL-10 in the intestinal mucosa, as well as an increase in the number of goblet cells. The anti-inflammatory cytokine miR146a was found to be increased in the ileum and brain. Furthermore, it increases the number of goblet cells which contribute to intestinal barrier integrity. Taken together, our findings reflect the ability of the tested bacterium to modulates the intestinal homeostasis and immune response. Detailed safety unpublished studies and genome data support our finding. The strain Rouxiella badensis subsp. acadiensis has been filed in a provisional patent; a U.S. Provisional Application No. 62/916,921 entitled “Probiotics Composition and Methods.” Future studies are still needed to validate the potential utilization of this strain as functional food and its potential probiotic effect. |
topic |
probiotic mucosal immunity immuno-modulation micoRNAs (miRNAs) gastro-intestinal tolerance |
url |
https://www.frontiersin.org/articles/10.3389/fmicb.2021.569119/full |
work_keys_str_mv |
AT nouryahfoufi immunomodulationandintestinalmorphofunctionalaspectsofanovelgramnegativebacteriumrouxiellabadensissubspacadiensis AT nawalalsadi immunomodulationandintestinalmorphofunctionalaspectsofanovelgramnegativebacteriumrouxiellabadensissubspacadiensis AT jeanfrancoismallet immunomodulationandintestinalmorphofunctionalaspectsofanovelgramnegativebacteriumrouxiellabadensissubspacadiensis AT garimakulshreshtha immunomodulationandintestinalmorphofunctionalaspectsofanovelgramnegativebacteriumrouxiellabadensissubspacadiensis AT maxwellhincke immunomodulationandintestinalmorphofunctionalaspectsofanovelgramnegativebacteriumrouxiellabadensissubspacadiensis AT maxwellhincke immunomodulationandintestinalmorphofunctionalaspectsofanovelgramnegativebacteriumrouxiellabadensissubspacadiensis AT nafissaismail immunomodulationandintestinalmorphofunctionalaspectsofanovelgramnegativebacteriumrouxiellabadensissubspacadiensis AT chantalmatar immunomodulationandintestinalmorphofunctionalaspectsofanovelgramnegativebacteriumrouxiellabadensissubspacadiensis AT chantalmatar immunomodulationandintestinalmorphofunctionalaspectsofanovelgramnegativebacteriumrouxiellabadensissubspacadiensis |
_version_ |
1721363551110561792 |