Lymph Node Subcapsular Sinus Microenvironment-On-A-Chip Modeling Shear Flow Relevant to Lymphatic Metastasis and Immune Cell Homing

Summary: A lymph node sinus-on-a-chip adhesion microfluidic platform that recapitulates the hydrodynamic microenvironment of the lymph node subcapsular sinus was engineered. This device was used to interrogate the effects of lymph node remodeling on cellular adhesion in fluid flow relevant to lympha...

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Main Authors:	Katherine G. Birmingham, Meghan J. O'Melia, Samantha Bordy, David Reyes Aguilar, Bassel El-Reyas, Gregory Lesinski, Susan N. Thomas
Format:	Article
Language:	English
Published:	Elsevier 2020-11-01
Series:	iScience
Subjects:	Tissue Engineering Cancer Bioengineering
Online Access:	http://www.sciencedirect.com/science/article/pii/S2589004220309482

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spelling	doaj-0d1706a2b93d47d7af57dd64d649ac942020-11-25T04:06:19ZengElsevieriScience2589-00422020-11-012311101751Lymph Node Subcapsular Sinus Microenvironment-On-A-Chip Modeling Shear Flow Relevant to Lymphatic Metastasis and Immune Cell HomingKatherine G. Birmingham0Meghan J. O'Melia1Samantha Bordy2David Reyes Aguilar3Bassel El-Reyas4Gregory Lesinski5Susan N. Thomas6George W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA; Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, IBB 2310 315 Ferst Drive NW, Atlanta, GA 30332, USAWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USAGeorge W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USAGeorge W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA; Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, IBB 2310 315 Ferst Drive NW, Atlanta, GA 30332, USAWinship Cancer Institute, Emory University, Atlanta, GA 30322, USAWinship Cancer Institute, Emory University, Atlanta, GA 30322, USAGeorge W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA; Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, IBB 2310 315 Ferst Drive NW, Atlanta, GA 30332, USA; Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA; Winship Cancer Institute, Emory University, Atlanta, GA 30322, USA; Corresponding authorSummary: A lymph node sinus-on-a-chip adhesion microfluidic platform that recapitulates the hydrodynamic microenvironment of the lymph node subcapsular sinus was engineered. This device was used to interrogate the effects of lymph node remodeling on cellular adhesion in fluid flow relevant to lymphatic metastasis. Wall shear stress levels analytically estimated and modeled after quiescent and diseased/inflamed lymph nodes were experimentally recapitulated using a flow-based microfluidic perfusion system to assess the effects of physiological flow fields on human metastatic cancer cell adhesion. Results suggest that both altered fluid flow profiles and presentation of adhesive ligands, which are predicted to manifest within the lymph node subcapsular sinus as a result of inflammation-induced remodeling, and the presence of lymph-borne monocytic cells may synergistically contribute to the dynamic extent of cell adhesion in flow relevant to lymph node invasion by cancer and monocytic immune cells during lymphatic metastasis.http://www.sciencedirect.com/science/article/pii/S2589004220309482Tissue EngineeringCancerBioengineering
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Katherine G. Birmingham Meghan J. O'Melia Samantha Bordy David Reyes Aguilar Bassel El-Reyas Gregory Lesinski Susan N. Thomas
spellingShingle	Katherine G. Birmingham Meghan J. O'Melia Samantha Bordy David Reyes Aguilar Bassel El-Reyas Gregory Lesinski Susan N. Thomas Lymph Node Subcapsular Sinus Microenvironment-On-A-Chip Modeling Shear Flow Relevant to Lymphatic Metastasis and Immune Cell Homing iScience Tissue Engineering Cancer Bioengineering
author_facet	Katherine G. Birmingham Meghan J. O'Melia Samantha Bordy David Reyes Aguilar Bassel El-Reyas Gregory Lesinski Susan N. Thomas
author_sort	Katherine G. Birmingham
title	Lymph Node Subcapsular Sinus Microenvironment-On-A-Chip Modeling Shear Flow Relevant to Lymphatic Metastasis and Immune Cell Homing
title_short	Lymph Node Subcapsular Sinus Microenvironment-On-A-Chip Modeling Shear Flow Relevant to Lymphatic Metastasis and Immune Cell Homing
title_full	Lymph Node Subcapsular Sinus Microenvironment-On-A-Chip Modeling Shear Flow Relevant to Lymphatic Metastasis and Immune Cell Homing
title_fullStr	Lymph Node Subcapsular Sinus Microenvironment-On-A-Chip Modeling Shear Flow Relevant to Lymphatic Metastasis and Immune Cell Homing
title_full_unstemmed	Lymph Node Subcapsular Sinus Microenvironment-On-A-Chip Modeling Shear Flow Relevant to Lymphatic Metastasis and Immune Cell Homing
title_sort	lymph node subcapsular sinus microenvironment-on-a-chip modeling shear flow relevant to lymphatic metastasis and immune cell homing
publisher	Elsevier
series	iScience
issn	2589-0042
publishDate	2020-11-01
description	Summary: A lymph node sinus-on-a-chip adhesion microfluidic platform that recapitulates the hydrodynamic microenvironment of the lymph node subcapsular sinus was engineered. This device was used to interrogate the effects of lymph node remodeling on cellular adhesion in fluid flow relevant to lymphatic metastasis. Wall shear stress levels analytically estimated and modeled after quiescent and diseased/inflamed lymph nodes were experimentally recapitulated using a flow-based microfluidic perfusion system to assess the effects of physiological flow fields on human metastatic cancer cell adhesion. Results suggest that both altered fluid flow profiles and presentation of adhesive ligands, which are predicted to manifest within the lymph node subcapsular sinus as a result of inflammation-induced remodeling, and the presence of lymph-borne monocytic cells may synergistically contribute to the dynamic extent of cell adhesion in flow relevant to lymph node invasion by cancer and monocytic immune cells during lymphatic metastasis.
topic	Tissue Engineering Cancer Bioengineering
url	http://www.sciencedirect.com/science/article/pii/S2589004220309482
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Lymph Node Subcapsular Sinus Microenvironment-On-A-Chip Modeling Shear Flow Relevant to Lymphatic Metastasis and Immune Cell Homing

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