Classical and molecular cytogenetic analysis in head and neck squamous cell carcinomas
Head and neck carcinomas represent the sixth most frequent type of cancer in the world, and 90% are derived from squamous cells (HNSCC). In this study of 15 HNSCC cases, extensive aneuploidy was detected by G banding in most tumors. The most frequently observed numerical changes involved gain of a c...
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Sociedade Brasileira de Genética
2003-01-01
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doaj-0d17b76b22754ddeb9f18455942b23612020-11-25T02:18:00ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852003-01-0126212112810.1590/S1415-47572003000200003Classical and molecular cytogenetic analysis in head and neck squamous cell carcinomasLuciana CS VeigaNádia A BérgamoLuiz Paulo KowalskiSilvia R RogattoHead and neck carcinomas represent the sixth most frequent type of cancer in the world, and 90% are derived from squamous cells (HNSCC). In this study of 15 HNSCC cases, extensive aneuploidy was detected by G banding in most tumors. The most frequently observed numerical changes involved gain of a chromosome 22, and loss of chromosomes Y, 10, 17, and 19. The most frequent structural alteration was del(22)(q13.1). As compared to G-banding, fluorescence in situ hybridization (FISH) proved to be an effective technique for detecting aneuploidy. Interphase FISH with a chromosome 17 centromere probe disclosed a high frequency of monosomy for chromosome 17, in contrast with G-banding, by which clonal monosomy 17 was detected in only three of the tumors. Painting probes for chromosomes 5 and 16 were used to evaluate a selected series of HNSCC in which G-banding analysis had shown marker chromosomes. FISH analysis failed to confirm the origin of the marker chromosomes, but four out of five cases showed a significant loss of chromosomes 5. This difference between FISH and G-banding results may reflect the smaller number of metaphase analyzed as well as the criteria adopted for sorting these metaphases. Therefore results obtained solely by G-banding analysis should be considered with caution. Our data confirmed the involvement of chromosome 17 in head and neck squamous cell carcinomas.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200003FISHchromosomal aberrationshead and neck cancerchromosome 17 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Luciana CS Veiga Nádia A Bérgamo Luiz Paulo Kowalski Silvia R Rogatto |
spellingShingle |
Luciana CS Veiga Nádia A Bérgamo Luiz Paulo Kowalski Silvia R Rogatto Classical and molecular cytogenetic analysis in head and neck squamous cell carcinomas Genetics and Molecular Biology FISH chromosomal aberrations head and neck cancer chromosome 17 |
author_facet |
Luciana CS Veiga Nádia A Bérgamo Luiz Paulo Kowalski Silvia R Rogatto |
author_sort |
Luciana CS Veiga |
title |
Classical and molecular cytogenetic analysis in head and neck squamous cell carcinomas |
title_short |
Classical and molecular cytogenetic analysis in head and neck squamous cell carcinomas |
title_full |
Classical and molecular cytogenetic analysis in head and neck squamous cell carcinomas |
title_fullStr |
Classical and molecular cytogenetic analysis in head and neck squamous cell carcinomas |
title_full_unstemmed |
Classical and molecular cytogenetic analysis in head and neck squamous cell carcinomas |
title_sort |
classical and molecular cytogenetic analysis in head and neck squamous cell carcinomas |
publisher |
Sociedade Brasileira de Genética |
series |
Genetics and Molecular Biology |
issn |
1415-4757 1678-4685 |
publishDate |
2003-01-01 |
description |
Head and neck carcinomas represent the sixth most frequent type of cancer in the world, and 90% are derived from squamous cells (HNSCC). In this study of 15 HNSCC cases, extensive aneuploidy was detected by G banding in most tumors. The most frequently observed numerical changes involved gain of a chromosome 22, and loss of chromosomes Y, 10, 17, and 19. The most frequent structural alteration was del(22)(q13.1). As compared to G-banding, fluorescence in situ hybridization (FISH) proved to be an effective technique for detecting aneuploidy. Interphase FISH with a chromosome 17 centromere probe disclosed a high frequency of monosomy for chromosome 17, in contrast with G-banding, by which clonal monosomy 17 was detected in only three of the tumors. Painting probes for chromosomes 5 and 16 were used to evaluate a selected series of HNSCC in which G-banding analysis had shown marker chromosomes. FISH analysis failed to confirm the origin of the marker chromosomes, but four out of five cases showed a significant loss of chromosomes 5. This difference between FISH and G-banding results may reflect the smaller number of metaphase analyzed as well as the criteria adopted for sorting these metaphases. Therefore results obtained solely by G-banding analysis should be considered with caution. Our data confirmed the involvement of chromosome 17 in head and neck squamous cell carcinomas. |
topic |
FISH chromosomal aberrations head and neck cancer chromosome 17 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200003 |
work_keys_str_mv |
AT lucianacsveiga classicalandmolecularcytogeneticanalysisinheadandnecksquamouscellcarcinomas AT nadiaabergamo classicalandmolecularcytogeneticanalysisinheadandnecksquamouscellcarcinomas AT luizpaulokowalski classicalandmolecularcytogeneticanalysisinheadandnecksquamouscellcarcinomas AT silviarrogatto classicalandmolecularcytogeneticanalysisinheadandnecksquamouscellcarcinomas |
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