MiR-183-5p Promotes Tumor Progression of Osteosarcoma and Predicts Poor Prognosis in Patients
Lin Jin, Yue Luo, Ying-Chun Zhao, Hai Tao Department of Orthopaedics, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, People’s Republic of ChinaCorrespondence: Hai TaoDepartment of Orthopaedics, Renmin Hospital of Wuhan University, Zhang Zhidong Road 99, Wuhan, Hubei 430060, Peop...
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doaj-0d2d416e156a42f29a302aaaecc86b672021-01-28T20:09:23ZengDove Medical PressCancer Management and Research1179-13222021-01-01Volume 1380581461534MiR-183-5p Promotes Tumor Progression of Osteosarcoma and Predicts Poor Prognosis in PatientsJin LLuo YZhao YCTao HLin Jin, Yue Luo, Ying-Chun Zhao, Hai Tao Department of Orthopaedics, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, People’s Republic of ChinaCorrespondence: Hai TaoDepartment of Orthopaedics, Renmin Hospital of Wuhan University, Zhang Zhidong Road 99, Wuhan, Hubei 430060, People’s Republic of ChinaEmail HaitaoW428@163.comBackground: Although miRNA-183-5p plays a critical role in many cancer types, including gastric cancer, hepatocellular carcinoma, prostate cancer, renal cell cancer and breast cancer, its role in osteosarcoma remains unclear.Methods: Expression levels of miR-183-5p were detected in osteosarcoma tissues and cell lines using qRT-PCR. The effect of miR-183-5p on the survival and recurrence of osteosarcoma patients was analyzed in a cohort of 80 patients using Kaplan–Meier curves and Cox regression analysis. Effects of miR-183-5p on cell proliferation, migration and invasion abilities were evaluated using CCK-8, crystal violet and transwell assays.Results: The expression of miR-183-5p was found to be upregulated in human osteosarcoma tissues and cell lines. Moreover, miR-183-5p expression was observed to be closely associated with tumor size, TNM stage and lung metastasis. Notably, high expression of miR-183-5p was shown to be able to predict unfavorable clinical prognosis in osteosarcoma patients. Additionally, whilst overexpression of miR-183-5p was observed to significantly promote the proliferation, migration and invasion of osteosarcoma cells; an inhibitory effect was observed with knockdown of miR-183-5p.Conclusion: This study demonstrated that miR-183-5p acts as an oncogene and plays a critical role in the regulation of osteosarcoma tumor progression, and our results suggest a novel potential prognostic and therapeutic value of miR-183-5p in osteosarcoma.Keywords: miR-183-5p, osteosarcoma, progression, prognosis, biomarkerhttps://www.dovepress.com/mir-183-5p-promotes-tumor-progression-of-osteosarcoma-and-predicts-poo-peer-reviewed-article-CMARmir-183-5posteosarcomaprogressionprognosisbiomarker |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jin L Luo Y Zhao YC Tao H |
spellingShingle |
Jin L Luo Y Zhao YC Tao H MiR-183-5p Promotes Tumor Progression of Osteosarcoma and Predicts Poor Prognosis in Patients Cancer Management and Research mir-183-5p osteosarcoma progression prognosis biomarker |
author_facet |
Jin L Luo Y Zhao YC Tao H |
author_sort |
Jin L |
title |
MiR-183-5p Promotes Tumor Progression of Osteosarcoma and Predicts Poor Prognosis in Patients |
title_short |
MiR-183-5p Promotes Tumor Progression of Osteosarcoma and Predicts Poor Prognosis in Patients |
title_full |
MiR-183-5p Promotes Tumor Progression of Osteosarcoma and Predicts Poor Prognosis in Patients |
title_fullStr |
MiR-183-5p Promotes Tumor Progression of Osteosarcoma and Predicts Poor Prognosis in Patients |
title_full_unstemmed |
MiR-183-5p Promotes Tumor Progression of Osteosarcoma and Predicts Poor Prognosis in Patients |
title_sort |
mir-183-5p promotes tumor progression of osteosarcoma and predicts poor prognosis in patients |
publisher |
Dove Medical Press |
series |
Cancer Management and Research |
issn |
1179-1322 |
publishDate |
2021-01-01 |
description |
Lin Jin, Yue Luo, Ying-Chun Zhao, Hai Tao Department of Orthopaedics, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, People’s Republic of ChinaCorrespondence: Hai TaoDepartment of Orthopaedics, Renmin Hospital of Wuhan University, Zhang Zhidong Road 99, Wuhan, Hubei 430060, People’s Republic of ChinaEmail HaitaoW428@163.comBackground: Although miRNA-183-5p plays a critical role in many cancer types, including gastric cancer, hepatocellular carcinoma, prostate cancer, renal cell cancer and breast cancer, its role in osteosarcoma remains unclear.Methods: Expression levels of miR-183-5p were detected in osteosarcoma tissues and cell lines using qRT-PCR. The effect of miR-183-5p on the survival and recurrence of osteosarcoma patients was analyzed in a cohort of 80 patients using Kaplan–Meier curves and Cox regression analysis. Effects of miR-183-5p on cell proliferation, migration and invasion abilities were evaluated using CCK-8, crystal violet and transwell assays.Results: The expression of miR-183-5p was found to be upregulated in human osteosarcoma tissues and cell lines. Moreover, miR-183-5p expression was observed to be closely associated with tumor size, TNM stage and lung metastasis. Notably, high expression of miR-183-5p was shown to be able to predict unfavorable clinical prognosis in osteosarcoma patients. Additionally, whilst overexpression of miR-183-5p was observed to significantly promote the proliferation, migration and invasion of osteosarcoma cells; an inhibitory effect was observed with knockdown of miR-183-5p.Conclusion: This study demonstrated that miR-183-5p acts as an oncogene and plays a critical role in the regulation of osteosarcoma tumor progression, and our results suggest a novel potential prognostic and therapeutic value of miR-183-5p in osteosarcoma.Keywords: miR-183-5p, osteosarcoma, progression, prognosis, biomarker |
topic |
mir-183-5p osteosarcoma progression prognosis biomarker |
url |
https://www.dovepress.com/mir-183-5p-promotes-tumor-progression-of-osteosarcoma-and-predicts-poo-peer-reviewed-article-CMAR |
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