Overexpression of the Wild Soybean R2R3-MYB Transcription Factor <i>GsMYB15</i> Enhances Resistance to Salt Stress and <i>Helicoverpa Armigera</i> in Transgenic <i>Arabidopsis</i>
Plant R2R3-MYB transcription factors (TFs) have been suggested to play crucial roles in the response to diverse abiotic and biotic stress factors but there is little molecular evidence of this role in soybean plants. In this work, we identified and functionally characterized an R2R3-MYB TF, namely,...
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doaj-0d31812d591a4f35a40e19407b22ae8c2020-11-24T21:41:36ZengMDPI AGInternational Journal of Molecular Sciences1422-00672018-12-011912395810.3390/ijms19123958ijms19123958Overexpression of the Wild Soybean R2R3-MYB Transcription Factor <i>GsMYB15</i> Enhances Resistance to Salt Stress and <i>Helicoverpa Armigera</i> in Transgenic <i>Arabidopsis</i>Xin-Jie Shen0Yan-Yan Wang1Yong-Xing Zhang2Wei Guo3Yong-Qing Jiao4Xin-An Zhou5Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaKey Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaKey Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaKey Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaKey Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaKey Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaPlant R2R3-MYB transcription factors (TFs) have been suggested to play crucial roles in the response to diverse abiotic and biotic stress factors but there is little molecular evidence of this role in soybean plants. In this work, we identified and functionally characterized an R2R3-MYB TF, namely, <i>GsMYB15</i>, from the wild soybean ED059. Protein and promoter sequence analysis indicated that <i>GsMYB15</i> is a typical R2R3-MYB TF and contains multiple stress-related <i>cis-</i>elements in the promoter region. <i>GsMYB15</i> is located in the nucleus and exhibits transcriptional activation activity. QPCR assays suggested that the expression of <i>GsMYB15</i> could be induced by NaCl, insect attacks and defense-related hormones (MeJA and SA). Furthermore, <i>GsMYB15</i> exhibited highest expression in pods compared to other tissues. Functional analysis of <i>GsMYB15</i> demonstrated that overexpression of <i>GsMYB15</i> could increase salt tolerance and enhance the resistance to <i>H. armigera larvae</i> in transgenic <i>Arabidopsis</i> plants. Moreover, overexpression of <i>GsMYB15</i> also affected the expression levels of salt stress- and defense-related genes in the transgenic plants. Feeding with transgenic <i>Arabidopsis</i> plant leaves could significantly suppress the expression levels of immunity-related genes in <i>H. armigera larvae</i>. Overexpression of <i>GsMYB15</i> also increased mesophyll cell levels in transgenic plants. Taken together, these results provide evidence that <i>GsMYB15</i> is a positive regulator of salt stress tolerance and insect resistance in transformed <i>Arabidopsis</i> plants.https://www.mdpi.com/1422-0067/19/12/3958R2R3-MYB transcription factorwild soybean<i>Helicoverpa armigera</i>salt stress<i><i>Arabidopsis</i> thaliana</i> |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xin-Jie Shen Yan-Yan Wang Yong-Xing Zhang Wei Guo Yong-Qing Jiao Xin-An Zhou |
spellingShingle |
Xin-Jie Shen Yan-Yan Wang Yong-Xing Zhang Wei Guo Yong-Qing Jiao Xin-An Zhou Overexpression of the Wild Soybean R2R3-MYB Transcription Factor <i>GsMYB15</i> Enhances Resistance to Salt Stress and <i>Helicoverpa Armigera</i> in Transgenic <i>Arabidopsis</i> International Journal of Molecular Sciences R2R3-MYB transcription factor wild soybean <i>Helicoverpa armigera</i> salt stress <i><i>Arabidopsis</i> thaliana</i> |
author_facet |
Xin-Jie Shen Yan-Yan Wang Yong-Xing Zhang Wei Guo Yong-Qing Jiao Xin-An Zhou |
author_sort |
Xin-Jie Shen |
title |
Overexpression of the Wild Soybean R2R3-MYB Transcription Factor <i>GsMYB15</i> Enhances Resistance to Salt Stress and <i>Helicoverpa Armigera</i> in Transgenic <i>Arabidopsis</i> |
title_short |
Overexpression of the Wild Soybean R2R3-MYB Transcription Factor <i>GsMYB15</i> Enhances Resistance to Salt Stress and <i>Helicoverpa Armigera</i> in Transgenic <i>Arabidopsis</i> |
title_full |
Overexpression of the Wild Soybean R2R3-MYB Transcription Factor <i>GsMYB15</i> Enhances Resistance to Salt Stress and <i>Helicoverpa Armigera</i> in Transgenic <i>Arabidopsis</i> |
title_fullStr |
Overexpression of the Wild Soybean R2R3-MYB Transcription Factor <i>GsMYB15</i> Enhances Resistance to Salt Stress and <i>Helicoverpa Armigera</i> in Transgenic <i>Arabidopsis</i> |
title_full_unstemmed |
Overexpression of the Wild Soybean R2R3-MYB Transcription Factor <i>GsMYB15</i> Enhances Resistance to Salt Stress and <i>Helicoverpa Armigera</i> in Transgenic <i>Arabidopsis</i> |
title_sort |
overexpression of the wild soybean r2r3-myb transcription factor <i>gsmyb15</i> enhances resistance to salt stress and <i>helicoverpa armigera</i> in transgenic <i>arabidopsis</i> |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2018-12-01 |
description |
Plant R2R3-MYB transcription factors (TFs) have been suggested to play crucial roles in the response to diverse abiotic and biotic stress factors but there is little molecular evidence of this role in soybean plants. In this work, we identified and functionally characterized an R2R3-MYB TF, namely, <i>GsMYB15</i>, from the wild soybean ED059. Protein and promoter sequence analysis indicated that <i>GsMYB15</i> is a typical R2R3-MYB TF and contains multiple stress-related <i>cis-</i>elements in the promoter region. <i>GsMYB15</i> is located in the nucleus and exhibits transcriptional activation activity. QPCR assays suggested that the expression of <i>GsMYB15</i> could be induced by NaCl, insect attacks and defense-related hormones (MeJA and SA). Furthermore, <i>GsMYB15</i> exhibited highest expression in pods compared to other tissues. Functional analysis of <i>GsMYB15</i> demonstrated that overexpression of <i>GsMYB15</i> could increase salt tolerance and enhance the resistance to <i>H. armigera larvae</i> in transgenic <i>Arabidopsis</i> plants. Moreover, overexpression of <i>GsMYB15</i> also affected the expression levels of salt stress- and defense-related genes in the transgenic plants. Feeding with transgenic <i>Arabidopsis</i> plant leaves could significantly suppress the expression levels of immunity-related genes in <i>H. armigera larvae</i>. Overexpression of <i>GsMYB15</i> also increased mesophyll cell levels in transgenic plants. Taken together, these results provide evidence that <i>GsMYB15</i> is a positive regulator of salt stress tolerance and insect resistance in transformed <i>Arabidopsis</i> plants. |
topic |
R2R3-MYB transcription factor wild soybean <i>Helicoverpa armigera</i> salt stress <i><i>Arabidopsis</i> thaliana</i> |
url |
https://www.mdpi.com/1422-0067/19/12/3958 |
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