Strategy for chemotherapeutic delivery using a nanosized porous metal-organic framework with a central composite design
Yingpeng Li,1 Xiuyan Li,2 Qingxia Guan,2 Chunjing Zhang,2 Ting Xu,2 Yujing Dong,2 Xinyu Bai,2 Weiping Zhang3 1College of Pharmacy, Tianjin University of Traditional Chinese Medicine, Tianjin, 2College of Pharmacy, Heilongjiang University of Traditional Chinese Medicine, Harbin, People’s R...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Dove Medical Press
2017-02-01
|
Series: | International Journal of Nanomedicine |
Subjects: | |
Online Access: | https://www.dovepress.com/strategy-for-chemotherapeutic-delivery-using-a-nanosized-porous-metal--peer-reviewed-article-IJN |
id |
doaj-0d39da1feb6d4579b0a8b8c585a4a6d9 |
---|---|
record_format |
Article |
spelling |
doaj-0d39da1feb6d4579b0a8b8c585a4a6d92020-11-25T00:47:25ZengDove Medical PressInternational Journal of Nanomedicine1178-20132017-02-01Volume 121465147431489Strategy for chemotherapeutic delivery using a nanosized porous metal-organic framework with a central composite designLi YPLi XYGuan QXZhang CJXu TDong YJBai XYZhang WPYingpeng Li,1 Xiuyan Li,2 Qingxia Guan,2 Chunjing Zhang,2 Ting Xu,2 Yujing Dong,2 Xinyu Bai,2 Weiping Zhang3 1College of Pharmacy, Tianjin University of Traditional Chinese Medicine, Tianjin, 2College of Pharmacy, Heilongjiang University of Traditional Chinese Medicine, Harbin, People’s Republic of China; 3Pope John XXIII High School, Everett, MA, USA Background: Enhancing drug delivery is an ongoing endeavor in pharmaceutics, especially when the efficacy of chemotherapy for cancer is concerned. In this study, we prepared and evaluated nanosized HKUST-1 (nanoHKUST-1), nanosized metal-organic drug delivery framework, loaded with 5-fluorouracil (5-FU) for potential use in cancer treatment.Materials and methods: NanoHKUST-1 was prepared by reacting copper (II) acetate [Cu(OAc)2] and benzene-1,3,5-tricarboxylic acid (H3BTC) with benzoic acid (C6H5COOH) at room temperature (23.7°C±2.4°C). A central composite design was used to optimize 5-FU-loaded nanoHKUST-1. Contact time, ethanol concentration, and 5-FU:material ratios were the independent variables, and the entrapment efficiency of 5-FU was the response parameter measured. Powder X-ray diffraction, scanning electron microscopy (SEM), transmission electron microscopy (TEM), and nitrogen adsorption were used to determine the morphology of nanoHKUST-1. In addition, 5-FU release studies were conducted, and the in vitro cytotoxicity was evaluated.Results: Entrapment efficiency and drug loading were 9.96% and 40.22%, respectively, while the small-angle X-ray diffraction patterns confirmed a regular porous structure. The SEM and TEM images of the nanoHKUST-1 confirmed the presence of round particles (diameter: approximately 100 nm) and regular polygon arrays of mesoporous channels of approximately 2–5 nm. The half-maximal lethal concentration (LC50) of the 5-FU-loaded nanoHKUST-1 was approximately 10 µg/mL.Conclusion: The results indicated that nanoHKUST-1 is a potential vector worth developing as a cancer chemotherapeutic drug delivery system. Keywords: 5-fluorouracil, drug delivery, nanoparticles, nano-MOFshttps://www.dovepress.com/strategy-for-chemotherapeutic-delivery-using-a-nanosized-porous-metal--peer-reviewed-article-IJN5-fluorouracildrug deliverynanoparticlesnanoMOFs |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Li YP Li XY Guan QX Zhang CJ Xu T Dong YJ Bai XY Zhang WP |
spellingShingle |
Li YP Li XY Guan QX Zhang CJ Xu T Dong YJ Bai XY Zhang WP Strategy for chemotherapeutic delivery using a nanosized porous metal-organic framework with a central composite design International Journal of Nanomedicine 5-fluorouracil drug delivery nanoparticles nanoMOFs |
author_facet |
Li YP Li XY Guan QX Zhang CJ Xu T Dong YJ Bai XY Zhang WP |
author_sort |
Li YP |
title |
Strategy for chemotherapeutic delivery using a nanosized porous metal-organic framework with a central composite design |
title_short |
Strategy for chemotherapeutic delivery using a nanosized porous metal-organic framework with a central composite design |
title_full |
Strategy for chemotherapeutic delivery using a nanosized porous metal-organic framework with a central composite design |
title_fullStr |
Strategy for chemotherapeutic delivery using a nanosized porous metal-organic framework with a central composite design |
title_full_unstemmed |
Strategy for chemotherapeutic delivery using a nanosized porous metal-organic framework with a central composite design |
title_sort |
strategy for chemotherapeutic delivery using a nanosized porous metal-organic framework with a central composite design |
publisher |
Dove Medical Press |
series |
International Journal of Nanomedicine |
issn |
1178-2013 |
publishDate |
2017-02-01 |
description |
Yingpeng Li,1 Xiuyan Li,2 Qingxia Guan,2 Chunjing Zhang,2 Ting Xu,2 Yujing Dong,2 Xinyu Bai,2 Weiping Zhang3 1College of Pharmacy, Tianjin University of Traditional Chinese Medicine, Tianjin, 2College of Pharmacy, Heilongjiang University of Traditional Chinese Medicine, Harbin, People’s Republic of China; 3Pope John XXIII High School, Everett, MA, USA Background: Enhancing drug delivery is an ongoing endeavor in pharmaceutics, especially when the efficacy of chemotherapy for cancer is concerned. In this study, we prepared and evaluated nanosized HKUST-1 (nanoHKUST-1), nanosized metal-organic drug delivery framework, loaded with 5-fluorouracil (5-FU) for potential use in cancer treatment.Materials and methods: NanoHKUST-1 was prepared by reacting copper (II) acetate [Cu(OAc)2] and benzene-1,3,5-tricarboxylic acid (H3BTC) with benzoic acid (C6H5COOH) at room temperature (23.7°C±2.4°C). A central composite design was used to optimize 5-FU-loaded nanoHKUST-1. Contact time, ethanol concentration, and 5-FU:material ratios were the independent variables, and the entrapment efficiency of 5-FU was the response parameter measured. Powder X-ray diffraction, scanning electron microscopy (SEM), transmission electron microscopy (TEM), and nitrogen adsorption were used to determine the morphology of nanoHKUST-1. In addition, 5-FU release studies were conducted, and the in vitro cytotoxicity was evaluated.Results: Entrapment efficiency and drug loading were 9.96% and 40.22%, respectively, while the small-angle X-ray diffraction patterns confirmed a regular porous structure. The SEM and TEM images of the nanoHKUST-1 confirmed the presence of round particles (diameter: approximately 100 nm) and regular polygon arrays of mesoporous channels of approximately 2–5 nm. The half-maximal lethal concentration (LC50) of the 5-FU-loaded nanoHKUST-1 was approximately 10 µg/mL.Conclusion: The results indicated that nanoHKUST-1 is a potential vector worth developing as a cancer chemotherapeutic drug delivery system. Keywords: 5-fluorouracil, drug delivery, nanoparticles, nano-MOFs |
topic |
5-fluorouracil drug delivery nanoparticles nanoMOFs |
url |
https://www.dovepress.com/strategy-for-chemotherapeutic-delivery-using-a-nanosized-porous-metal--peer-reviewed-article-IJN |
work_keys_str_mv |
AT liyp strategyforchemotherapeuticdeliveryusingananosizedporousmetalorganicframeworkwithacentralcompositedesign AT lixy strategyforchemotherapeuticdeliveryusingananosizedporousmetalorganicframeworkwithacentralcompositedesign AT guanqx strategyforchemotherapeuticdeliveryusingananosizedporousmetalorganicframeworkwithacentralcompositedesign AT zhangcj strategyforchemotherapeuticdeliveryusingananosizedporousmetalorganicframeworkwithacentralcompositedesign AT xut strategyforchemotherapeuticdeliveryusingananosizedporousmetalorganicframeworkwithacentralcompositedesign AT dongyj strategyforchemotherapeuticdeliveryusingananosizedporousmetalorganicframeworkwithacentralcompositedesign AT baixy strategyforchemotherapeuticdeliveryusingananosizedporousmetalorganicframeworkwithacentralcompositedesign AT zhangwp strategyforchemotherapeuticdeliveryusingananosizedporousmetalorganicframeworkwithacentralcompositedesign |
_version_ |
1725260091523858432 |