Protective effect of celastrol on type 2 diabetes mellitus with nonalcoholic fatty liver disease in mice
Abstract To investigate the protective effects of celastrol on mice with type 2 diabetes mellitus (T2DM) and nonalcoholic fatty liver disease (NAFLD), and to explore its underlying mechanism. The levels of low‐density lipoprotein cholesterol (LDL‐C), high‐density lipoprotein cholesterol (HDL‐C), tot...
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doaj-0d5675ad54b843f28f49caf0615c27ac2020-11-25T04:12:33ZengWileyFood Science & Nutrition2048-71772020-11-018116207621610.1002/fsn3.1917Protective effect of celastrol on type 2 diabetes mellitus with nonalcoholic fatty liver disease in miceJuanJuan Sun0Hui‐juan Wang1Jun Yu2TingTing Li3YiDi Han4The Second District of Hepatopathy Qingdao No. 6 People's Hospital Qingdao ChinaThe Second District of Hepatopathy Qingdao No. 6 People's Hospital Qingdao ChinaThe Second District of Hepatopathy Qingdao No. 6 People's Hospital Qingdao ChinaThe Second District of Hepatopathy Qingdao No. 6 People's Hospital Qingdao ChinaThe Second District of Hepatopathy Qingdao No. 6 People's Hospital Qingdao ChinaAbstract To investigate the protective effects of celastrol on mice with type 2 diabetes mellitus (T2DM) and nonalcoholic fatty liver disease (NAFLD), and to explore its underlying mechanism. The levels of low‐density lipoprotein cholesterol (LDL‐C), high‐density lipoprotein cholesterol (HDL‐C), total cholesterol (TC), and triglyceride (TG) in serum were tested. Malondialdehyde (MDA) and superoxide dismutase (SOD), GOT, and GPT in serum were also detected. The histopathological changes of liver tissues were observed by HE staining. The apoptosis cell number of liver tissues was measured by TUNEL staining. Nrf‐2 and HO‐1 protein and mRNA expression were evaluated by IHC, WB, and RT‐PCR assay. Celastrol had effects to depress TG, TC, LDL‐C, GPT, GOT, and MDA concentration and increase HDL‐C and SOD concentration (p < .05, respectively) with dose‐dependent. Compared with model group, apoptosis cell number was significantly depressed in Cel‐treated groups with dose‐dependent (p < .05, respectively). Nrf‐2 and HO‐1 mRNA and protein expressions were significantly improved in Cel‐treated groups with dose‐dependent (p < .05, respectively). Celastrol can inhibit the oxidative stress reaction and liver cell apoptosis via regulation Nrf2/HO‐1 pathway in T2DM mice with NAFLD.https://doi.org/10.1002/fsn3.1917celastrolcell apoptosisfatty liver diseasenonalcoholicNrf2/HO‐1 pathwaytype 2 diabetes mellitus |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
JuanJuan Sun Hui‐juan Wang Jun Yu TingTing Li YiDi Han |
spellingShingle |
JuanJuan Sun Hui‐juan Wang Jun Yu TingTing Li YiDi Han Protective effect of celastrol on type 2 diabetes mellitus with nonalcoholic fatty liver disease in mice Food Science & Nutrition celastrol cell apoptosis fatty liver disease nonalcoholic Nrf2/HO‐1 pathway type 2 diabetes mellitus |
author_facet |
JuanJuan Sun Hui‐juan Wang Jun Yu TingTing Li YiDi Han |
author_sort |
JuanJuan Sun |
title |
Protective effect of celastrol on type 2 diabetes mellitus with nonalcoholic fatty liver disease in mice |
title_short |
Protective effect of celastrol on type 2 diabetes mellitus with nonalcoholic fatty liver disease in mice |
title_full |
Protective effect of celastrol on type 2 diabetes mellitus with nonalcoholic fatty liver disease in mice |
title_fullStr |
Protective effect of celastrol on type 2 diabetes mellitus with nonalcoholic fatty liver disease in mice |
title_full_unstemmed |
Protective effect of celastrol on type 2 diabetes mellitus with nonalcoholic fatty liver disease in mice |
title_sort |
protective effect of celastrol on type 2 diabetes mellitus with nonalcoholic fatty liver disease in mice |
publisher |
Wiley |
series |
Food Science & Nutrition |
issn |
2048-7177 |
publishDate |
2020-11-01 |
description |
Abstract To investigate the protective effects of celastrol on mice with type 2 diabetes mellitus (T2DM) and nonalcoholic fatty liver disease (NAFLD), and to explore its underlying mechanism. The levels of low‐density lipoprotein cholesterol (LDL‐C), high‐density lipoprotein cholesterol (HDL‐C), total cholesterol (TC), and triglyceride (TG) in serum were tested. Malondialdehyde (MDA) and superoxide dismutase (SOD), GOT, and GPT in serum were also detected. The histopathological changes of liver tissues were observed by HE staining. The apoptosis cell number of liver tissues was measured by TUNEL staining. Nrf‐2 and HO‐1 protein and mRNA expression were evaluated by IHC, WB, and RT‐PCR assay. Celastrol had effects to depress TG, TC, LDL‐C, GPT, GOT, and MDA concentration and increase HDL‐C and SOD concentration (p < .05, respectively) with dose‐dependent. Compared with model group, apoptosis cell number was significantly depressed in Cel‐treated groups with dose‐dependent (p < .05, respectively). Nrf‐2 and HO‐1 mRNA and protein expressions were significantly improved in Cel‐treated groups with dose‐dependent (p < .05, respectively). Celastrol can inhibit the oxidative stress reaction and liver cell apoptosis via regulation Nrf2/HO‐1 pathway in T2DM mice with NAFLD. |
topic |
celastrol cell apoptosis fatty liver disease nonalcoholic Nrf2/HO‐1 pathway type 2 diabetes mellitus |
url |
https://doi.org/10.1002/fsn3.1917 |
work_keys_str_mv |
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