Analysis of Genomic Alteration in Primary Central Nervous System Lymphoma and the Expression of Some Related Genes
Primary central nervous system lymphoma (PCNSL) is a rare and special type of non-Hodgkin lymphoma. The treatment of PCNSL is comprehensive, combining surgery, radiotherapy, and chemotherapy. However, the outcome is poor because of its high invasiveness and rate of recurrence. We analyzed 22 cases o...
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Series: | Neoplasia: An International Journal for Oncology Research |
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doaj-0d5c4b8e96e54c8384099694430ef28e2020-11-24T21:30:05ZengElsevierNeoplasia: An International Journal for Oncology Research1476-55862018-10-01201010591069Analysis of Genomic Alteration in Primary Central Nervous System Lymphoma and the Expression of Some Related GenesYangying Zhou0Wei Liu1Zhijie Xu2Hong Zhu3Desheng Xiao4Weiping Su5Ruolan Zeng6Yuhua Feng7Yumei Duan8Jianhua Zhou9Meizuo Zhong10Department of Oncology, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, ChinaDepartment of Oncology, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, ChinaDepartment of Pathology, Xiangya Hospital, Central South University, Changsha 410008, Hunan, ChinaDepartment of Oncology, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, ChinaDepartment of Pathology, Xiangya Hospital, Central South University, Changsha 410008, Hunan, ChinaDepartment of Orthopedics, Xiangya Hospital, Central South University, Changsha 410008, Hunan, ChinaHunan Cancer Hospital and Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, 410013, Hunan, ChinaDepartment of Oncology, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, ChinaDepartment of Pathology, Xiangya Hospital, Central South University, Changsha 410008, Hunan, ChinaDepartment of Pathology, Xiangya Hospital, Central South University, Changsha 410008, Hunan, China; Address all correspondence to: Jianhua Zhou, Department of Pathology, Xiangya Hospital, Central South University, Changsha 410008, Hunan, China. or Meizuo Zhong, Department of Oncology, Xiangya Hospital, Central South University, Changsha, 410008, China.Department of Oncology, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China; Address all correspondence to: Jianhua Zhou, Department of Pathology, Xiangya Hospital, Central South University, Changsha 410008, Hunan, China. or Meizuo Zhong, Department of Oncology, Xiangya Hospital, Central South University, Changsha, 410008, China.Primary central nervous system lymphoma (PCNSL) is a rare and special type of non-Hodgkin lymphoma. The treatment of PCNSL is comprehensive, combining surgery, radiotherapy, and chemotherapy. However, the outcome is poor because of its high invasiveness and rate of recurrence. We analyzed 22 cases of PCNSL using next-generation sequencing (NGS) to detect 64 candidate genes. We used immunohistochemical methods to analyze gene expression in 57 PCNSL samples. NGS showed that recurrent mutations in KMT2D and CD79B, components of the NF-κB pathway, accounted for 65% of total mutations in PCNSL samples. The most frequent mutated gene was PIM1 (77.27%, 17/22), followed by MYD88 (63.64%, 14/22), CD79B (69.09%, 13/22), and KMT2D (50.00%, 11/22). Mutations of the CD79B gene were associated with an inferior progression-free survival (PFS), and GNA13 gene mutations were associated with a shorter PFS and overall survival (OS) in PCNSL patients (P < .05). PIM1 and MYD88 were highly expressed in PCNSL patients and were related to their OS time. MYD88 overexpression might be an independent and poor prognostic predictor of OS time. In summary, we identified highly recurrent genetic lesions in CD79B and KMT2D, components of the NF-κB pathway, in PCNSL and validated the expression of PIM1 and MYD88 related to poor survival, thereby providing novel insights into the pathogenesis and precision medicine of PCNSL.http://www.sciencedirect.com/science/article/pii/S1476558618302999 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yangying Zhou Wei Liu Zhijie Xu Hong Zhu Desheng Xiao Weiping Su Ruolan Zeng Yuhua Feng Yumei Duan Jianhua Zhou Meizuo Zhong |
spellingShingle |
Yangying Zhou Wei Liu Zhijie Xu Hong Zhu Desheng Xiao Weiping Su Ruolan Zeng Yuhua Feng Yumei Duan Jianhua Zhou Meizuo Zhong Analysis of Genomic Alteration in Primary Central Nervous System Lymphoma and the Expression of Some Related Genes Neoplasia: An International Journal for Oncology Research |
author_facet |
Yangying Zhou Wei Liu Zhijie Xu Hong Zhu Desheng Xiao Weiping Su Ruolan Zeng Yuhua Feng Yumei Duan Jianhua Zhou Meizuo Zhong |
author_sort |
Yangying Zhou |
title |
Analysis of Genomic Alteration in Primary Central Nervous System Lymphoma and the Expression of Some Related Genes |
title_short |
Analysis of Genomic Alteration in Primary Central Nervous System Lymphoma and the Expression of Some Related Genes |
title_full |
Analysis of Genomic Alteration in Primary Central Nervous System Lymphoma and the Expression of Some Related Genes |
title_fullStr |
Analysis of Genomic Alteration in Primary Central Nervous System Lymphoma and the Expression of Some Related Genes |
title_full_unstemmed |
Analysis of Genomic Alteration in Primary Central Nervous System Lymphoma and the Expression of Some Related Genes |
title_sort |
analysis of genomic alteration in primary central nervous system lymphoma and the expression of some related genes |
publisher |
Elsevier |
series |
Neoplasia: An International Journal for Oncology Research |
issn |
1476-5586 |
publishDate |
2018-10-01 |
description |
Primary central nervous system lymphoma (PCNSL) is a rare and special type of non-Hodgkin lymphoma. The treatment of PCNSL is comprehensive, combining surgery, radiotherapy, and chemotherapy. However, the outcome is poor because of its high invasiveness and rate of recurrence. We analyzed 22 cases of PCNSL using next-generation sequencing (NGS) to detect 64 candidate genes. We used immunohistochemical methods to analyze gene expression in 57 PCNSL samples. NGS showed that recurrent mutations in KMT2D and CD79B, components of the NF-κB pathway, accounted for 65% of total mutations in PCNSL samples. The most frequent mutated gene was PIM1 (77.27%, 17/22), followed by MYD88 (63.64%, 14/22), CD79B (69.09%, 13/22), and KMT2D (50.00%, 11/22). Mutations of the CD79B gene were associated with an inferior progression-free survival (PFS), and GNA13 gene mutations were associated with a shorter PFS and overall survival (OS) in PCNSL patients (P < .05). PIM1 and MYD88 were highly expressed in PCNSL patients and were related to their OS time. MYD88 overexpression might be an independent and poor prognostic predictor of OS time. In summary, we identified highly recurrent genetic lesions in CD79B and KMT2D, components of the NF-κB pathway, in PCNSL and validated the expression of PIM1 and MYD88 related to poor survival, thereby providing novel insights into the pathogenesis and precision medicine of PCNSL. |
url |
http://www.sciencedirect.com/science/article/pii/S1476558618302999 |
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