Los complejos Chaperonina(MSP63)inducen anticuerpos de reacciones cruzadas, bactericidas y opsonofagocítica.
Alteration of the native structure of antigens can lead to the loss of protective epitopes. Our previous results showed that separation of the meningococcal outer membrane proteins in native conditions revealed the existence of protein complexes that could be relevant for the development of new vacc...
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doaj-0d68ca7726df4611bc724a89dfa87e012020-11-24T23:25:33ZengFinlay EdicionesVacciMonitor1025-028X1025-02982009-08-011828587Los complejos Chaperonina(MSP63)inducen anticuerpos de reacciones cruzadas, bactericidas y opsonofagocítica.Juan Marzoa0Sandra Sánchez1Charlotte Brookes2Stephen Taylor3Andrew Gorringe4M. Teresa Criado5Carlos M. Ferreirós6University of Santiago de Compostela, Departamento de Microbiología y Parasitología, Facultad de Farmacia, Campus Sur, Universidad de Santiago de Compostela, 1782 Santiago de Compostela, SpainUniversity of Santiago de Compostela, Departamento de Microbiología y Parasitología, Facultad de Farmacia, Campus Sur, Universidad de Santiago de Compostela, 1782 Santiago de Compostela, SpainHealth Protection Agency, Centre for Emergency Preparedness and Response, Porton Down, Salisbury, UK.Health Protection Agency, Centre for Emergency Preparedness and Response, Porton Down, Salisbury, UK.Health Protection Agency, Centre for Emergency Preparedness and Response, Porton Down, Salisbury, UK.University of Santiago de Compostela, Departamento de Microbiología y Parasitología, Facultad de Farmacia, Campus Sur, Universidad de Santiago de Compostela, 1782 Santiago de Compostela, SpainUniversity of Santiago de Compostela, Departamento de Microbiología y Parasitología, Facultad de Farmacia, Campus Sur, Universidad de Santiago de Compostela, 1782 Santiago de Compostela, SpainAlteration of the native structure of antigens can lead to the loss of protective epitopes. Our previous results showed that separation of the meningococcal outer membrane proteins in native conditions revealed the existence of protein complexes that could be relevant for the development of new vaccine formulations. The aim of this work was to analyse the immunogenic characteristics of a highly conserved 700 kDa chaperonin complex (CxChap) detected and purified by using high resolution clear native electrophoresis. Analysis of the anti-CxChap serum by Western-blotting revealed the presence of antibodies against the MSP63 but also against the macrophage infectivity potentiator-like protein (MIP), which is coopurified with the chaperonin complex. Antibodies raised by immunisation with CxChap chaperonin complex show bactericidal and opsonophagocytic activity. http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1025-028X2009000200011&lng=es&nrm=iso&tlng=enNeisseria meningitidis complexesMSP63MIPhrCNEbactericidal activityopsonophagocytosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Juan Marzoa Sandra Sánchez Charlotte Brookes Stephen Taylor Andrew Gorringe M. Teresa Criado Carlos M. Ferreirós |
spellingShingle |
Juan Marzoa Sandra Sánchez Charlotte Brookes Stephen Taylor Andrew Gorringe M. Teresa Criado Carlos M. Ferreirós Los complejos Chaperonina(MSP63)inducen anticuerpos de reacciones cruzadas, bactericidas y opsonofagocítica. VacciMonitor Neisseria meningitidis complexes MSP63 MIP hrCNE bactericidal activity opsonophagocytosis |
author_facet |
Juan Marzoa Sandra Sánchez Charlotte Brookes Stephen Taylor Andrew Gorringe M. Teresa Criado Carlos M. Ferreirós |
author_sort |
Juan Marzoa |
title |
Los complejos Chaperonina(MSP63)inducen anticuerpos de reacciones cruzadas, bactericidas y opsonofagocítica. |
title_short |
Los complejos Chaperonina(MSP63)inducen anticuerpos de reacciones cruzadas, bactericidas y opsonofagocítica. |
title_full |
Los complejos Chaperonina(MSP63)inducen anticuerpos de reacciones cruzadas, bactericidas y opsonofagocítica. |
title_fullStr |
Los complejos Chaperonina(MSP63)inducen anticuerpos de reacciones cruzadas, bactericidas y opsonofagocítica. |
title_full_unstemmed |
Los complejos Chaperonina(MSP63)inducen anticuerpos de reacciones cruzadas, bactericidas y opsonofagocítica. |
title_sort |
los complejos chaperonina(msp63)inducen anticuerpos de reacciones cruzadas, bactericidas y opsonofagocítica. |
publisher |
Finlay Ediciones |
series |
VacciMonitor |
issn |
1025-028X 1025-0298 |
publishDate |
2009-08-01 |
description |
Alteration of the native structure of antigens can lead to the loss of protective epitopes. Our previous results showed that separation of the meningococcal outer membrane proteins in native conditions revealed the existence of protein complexes that could be relevant for the development of new vaccine formulations. The aim of this work was to analyse the immunogenic characteristics of a highly conserved 700 kDa chaperonin complex (CxChap) detected and purified by using high resolution clear native electrophoresis. Analysis of the anti-CxChap serum by Western-blotting revealed the presence of antibodies against the MSP63 but also against the macrophage infectivity potentiator-like protein (MIP), which is coopurified with the chaperonin complex. Antibodies raised by immunisation with CxChap chaperonin complex show bactericidal and opsonophagocytic activity. |
topic |
Neisseria meningitidis complexes MSP63 MIP hrCNE bactericidal activity opsonophagocytosis |
url |
http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1025-028X2009000200011&lng=es&nrm=iso&tlng=en |
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