Functional Analysis of Maize Silk-Specific ZmbZIP25 Promoter
ZmbZIP25 (Zea mays bZIP (basic leucine zipper) transcription factor 25) is a function-unknown protein that belongs to the D group of the bZIP transcription factor family. RNA-seq data showed that the expression of ZmbZIP25 was tissue-specific in maize silks, and this specificity was confirmed by RT-...
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doaj-0d77b7aa3a284015920014a297e7af9e2020-11-25T01:28:28ZengMDPI AGInternational Journal of Molecular Sciences1422-00672018-03-0119382210.3390/ijms19030822ijms19030822Functional Analysis of Maize Silk-Specific ZmbZIP25 PromoterWanying Li0Dan Yu1Jingjuan Yu2Dengyun Zhu3Qian Zhao4State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, ChinaState Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, ChinaState Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, ChinaState Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, ChinaState Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, ChinaZmbZIP25 (Zea mays bZIP (basic leucine zipper) transcription factor 25) is a function-unknown protein that belongs to the D group of the bZIP transcription factor family. RNA-seq data showed that the expression of ZmbZIP25 was tissue-specific in maize silks, and this specificity was confirmed by RT-PCR (reverse transcription-polymerase chain reaction). In situ RNA hybridization showed that ZmbZIP25 was expressed exclusively in the xylem of maize silks. A 5′ RACE (rapid amplification of cDNA ends) assay identified an adenine residue as the transcription start site of the ZmbZIP25 gene. To characterize this silk-specific promoter, we isolated and analyzed a 2450 bp (from −2083 to +367) and a 2600 bp sequence of ZmbZIP25 (from −2083 to +517, the transcription start site was denoted +1). Stable expression assays in Arabidopsis showed that the expression of the reporter gene GUS driven by the 2450 bp ZmbZIP25 5′-flanking fragment occurred exclusively in the papillae of Arabidopsis stigmas. Furthermore, transient expression assays in maize indicated that GUS and GFP expression driven by the 2450 bp ZmbZIP25 5′-flanking sequences occurred only in maize silks and not in other tissues. However, no GUS or GFP expression was driven by the 2600 bp ZmbZIP25 5′-flanking sequences in either stable or transient expression assays. A series of deletion analyses of the 2450 bp ZmbZIP25 5′-flanking sequence was performed in transgenic Arabidopsis plants, and probable elements prediction analysis revealed the possible presence of negative regulatory elements within the 161 bp region from −1117 to −957 that were responsible for the specificity of the ZmbZIP25 5′-flanking sequence.http://www.mdpi.com/1422-0067/19/3/822bZIP transcription factorintronmaizesilk specificity5′-flanking sequence |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wanying Li Dan Yu Jingjuan Yu Dengyun Zhu Qian Zhao |
spellingShingle |
Wanying Li Dan Yu Jingjuan Yu Dengyun Zhu Qian Zhao Functional Analysis of Maize Silk-Specific ZmbZIP25 Promoter International Journal of Molecular Sciences bZIP transcription factor intron maize silk specificity 5′-flanking sequence |
author_facet |
Wanying Li Dan Yu Jingjuan Yu Dengyun Zhu Qian Zhao |
author_sort |
Wanying Li |
title |
Functional Analysis of Maize Silk-Specific ZmbZIP25 Promoter |
title_short |
Functional Analysis of Maize Silk-Specific ZmbZIP25 Promoter |
title_full |
Functional Analysis of Maize Silk-Specific ZmbZIP25 Promoter |
title_fullStr |
Functional Analysis of Maize Silk-Specific ZmbZIP25 Promoter |
title_full_unstemmed |
Functional Analysis of Maize Silk-Specific ZmbZIP25 Promoter |
title_sort |
functional analysis of maize silk-specific zmbzip25 promoter |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2018-03-01 |
description |
ZmbZIP25 (Zea mays bZIP (basic leucine zipper) transcription factor 25) is a function-unknown protein that belongs to the D group of the bZIP transcription factor family. RNA-seq data showed that the expression of ZmbZIP25 was tissue-specific in maize silks, and this specificity was confirmed by RT-PCR (reverse transcription-polymerase chain reaction). In situ RNA hybridization showed that ZmbZIP25 was expressed exclusively in the xylem of maize silks. A 5′ RACE (rapid amplification of cDNA ends) assay identified an adenine residue as the transcription start site of the ZmbZIP25 gene. To characterize this silk-specific promoter, we isolated and analyzed a 2450 bp (from −2083 to +367) and a 2600 bp sequence of ZmbZIP25 (from −2083 to +517, the transcription start site was denoted +1). Stable expression assays in Arabidopsis showed that the expression of the reporter gene GUS driven by the 2450 bp ZmbZIP25 5′-flanking fragment occurred exclusively in the papillae of Arabidopsis stigmas. Furthermore, transient expression assays in maize indicated that GUS and GFP expression driven by the 2450 bp ZmbZIP25 5′-flanking sequences occurred only in maize silks and not in other tissues. However, no GUS or GFP expression was driven by the 2600 bp ZmbZIP25 5′-flanking sequences in either stable or transient expression assays. A series of deletion analyses of the 2450 bp ZmbZIP25 5′-flanking sequence was performed in transgenic Arabidopsis plants, and probable elements prediction analysis revealed the possible presence of negative regulatory elements within the 161 bp region from −1117 to −957 that were responsible for the specificity of the ZmbZIP25 5′-flanking sequence. |
topic |
bZIP transcription factor intron maize silk specificity 5′-flanking sequence |
url |
http://www.mdpi.com/1422-0067/19/3/822 |
work_keys_str_mv |
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